Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites

Love, Jennifer M.; Knight, Andrew M.; McAleer, Marcia A.; Todd, John A.

doi:10.1093/nar/18.14.4123

Cited by 437 publications

(185 citation statements)

References 27 publications

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“…When such regions, which have been termed 'microsatellites' (Litt and Luty, 1989), are individually amplified by means of the polymerase chain reaction (PCR) (Saiki et a/., 1988), using a pair of flanking unique oligonucleotides as primers, they almost invariably show extensive polymorphism due to site-specific length variation, as a consequence of the occurrence of different numbers of repeat units. This has been observed in diverse organisms, such as in humans (Edwards eta/., 1991;Litt and Luty, 1989;Tautz, 1989;Weber and May, 1989), whales (Schlotterer et a/., 1991;Tautz, 1989), Drosophila (Tautz, 1989), mice (Love et a/., 1990), cows and sheep (Moore etal., 1991). In plants, oligonucleotides containing TG and GATNGACA repeats have been shown to detect polymorphisms when used as probes in RFLP experiments (Lonn eta/., 1992;Weising eta/., 1989).…”

Section: Introductionmentioning

confidence: 91%

“…(dT-dG), (Hamada eta/., 1982). Many other microsatellite sequences have, however, been utilized as highly informative genetic markers and are currently employed in order to 'fill gaps' in the existing human and mouse genetic map and to improve map resolution (Edwards et a/., 1991;Garchon and Bach, 1992;Kidd, 1991;Love et a/., 1990;Nelson, 1991). The utilization of microsatellite sites as genetic markers has therefore been proposed as a general approach to genetic mapping of eukaryotes (Beckmann and Soller, 1990).…”

Section: Introductionmentioning

confidence: 99%

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PCR‐amplified microsatellites as markers in plant genetics

1993

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SummaryIn order to assess the feasibility of using microsatellites as markers in plant genetics, a survey of published DNA sequence data for presence, abundance and ubiquity in higher plants of all types of dinucleotide and trinucleotide repeats with a minimum number of 10 and 7 units, respectively, was conducted. This search revealed that such microsatellites are frequent and widely distributed; they were uncovered in 34 species, with a frequency of one every 50 kb. AT repeats were by far the most frequently observed class of dinucleotide microsatellites, whereas ACffG repeats, which are common in animals, were observed only once. TAT repeats prevailed among trinucleotides. Polymerase chain reaction amplification of (AT), and (TAT), microsatellites in soybean (Glycine max (L.) Merr.) revealed that they are highly polymorphic, as a consequence of length variation, somatically stable and inherited in a co-dominant Mendelian manner. The abundance and amount of information derived from such markers, together with the ease by which they can be identified, make them ideal markers for plant genetic linkage and physical mapping, population studies and varietal identification.

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Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

PCR‐amplified microsatellites as markers in plant genetics

1993

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“…SSLPs were detected by PCR amplification of genomic DNA samples. Primers specific for DllMitl, DllMitS, DllMit22, and Csfgm have been described (Love et al 1990). Amplification of M. castaneus and vt/vt genomic DNA samples produced products of 120 and 160 bp for DllMitl primers, 130 and 200 bp for DllMit5, and 270 and 240 bp for DllMit22, respectively.…”

Section: Genetic Mappingmentioning

confidence: 99%

Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development.

Greco¹,

Takada²,

Newhouse³

et al. 1996

Genes Dev.

234

161

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Mice homozygous for the recessive mutation vestigial tail (v-t), which arose spontaneously on Chromosome 11, exhibit vertebral abnormalities, including loss of caudal vertebrae leading to shortening of the tail. Wnt-3a, a member of the wingless family of secreted glycoproteins, maps to the same chromosome. Embryos homozygous for a null mutation in Wnt-3a (Wnt-3a "e°) have a complete absence of tail bud development and are truncated rostral to the hindlimbs. Several lines of evidence reveal that vt is a hypomorphic allele of Wnt-3a. We show that Wnt-3a and vt eosegregate in a high-resolution backcross and fail to complement, suggesting that Wnt-3a "~° and vt are allelic. Embryos heterozygous for both alleles have a phenotype intermediate between that of Wnt-3a n~° and vt homozygotes, lacking a tail, but developing thoracic and a variable number of lumbar vertebrae. Although no gross alteration in the Wnt-3a gene was detected in vt mice and the Wnt-3a coding region was normal, Wnt-3a expression was markedly reduced in vt/rt embryos consistent with a regulatory mutation in Wnt-3a. Furthermore, the analysis of allelic combinations indicates that Wnt-3a is required throughout the period of tail bud development for caudal somitogenesis. Interestingly, increasing levels of Wnt-3a activity appear to be necessary for the formation of more posterior derivatives of the paraxial mesoderm.

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“…One way is to amplify short repetitive sequences in the genome and compare the size of the repeats in the two strains of mice being used in the mapping cross. This is similar to how the original markers (MIT markers) were generated (Love et al, 1990); however, instead of generating random markers and then locating them in the genome, researchers now can locate a repeat and test whether it is polymorphic. This saves time as the researcher only generates new markers that will help them locate the mutation they are studying.…”

Section: The Powers Of the Sequencementioning

confidence: 84%

Uncovering the uncharacterized and unexpected: Unbiased phenotype‐driven screens in the mouse

Caspary

Anderson

2006

Developmental Dynamics

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Phenotype-based chemical mutagenesis screens for mouse mutations have undergone a transformation in the past five years from a potential approach to a practical tool. This change has been driven by the relative ease of identifying causative mutations now that the complete genome sequence is available. These unbiased screens make it possible to identify genes, gene functions and processes that are uniquely important to mammals. In addition, because chemical mutagenesis generally induces point mutations, these alleles often uncover previously unappreciated functions of known proteins. Here we provide examples of the success stories from forward genetic screens, emphasizing the examples that illustrate the discovery of mammalian-specific processes that could not be discovered in other model organisms. As the efficiency of sequencing and mutation detection continues to improve, it is likely that forward genetic screens will provide an even more important part of the repertoire of mouse genetics in the future. Developmental Dynamics 235:2412-2423, 2006.

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Towards construction of a high resolution map of the mouse genome using PCR-analysed microsatellites

Cited by 437 publications

References 27 publications

PCR‐amplified microsatellites as markers in plant genetics

PCR‐amplified microsatellites as markers in plant genetics

Analysis of the vestigial tail mutation demonstrates that Wnt-3a gene dosage regulates mouse axial development.

Uncovering the uncharacterized and unexpected: Unbiased phenotype‐driven screens in the mouse

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