Towards breeding of rapeseed (&lt;i&gt;Brassica napus&lt;/i&gt;) with alien cytoplasm and powdery mildew resistance from Ethiopian mustard (&lt;i&gt;Brassica carinata&lt;/i&gt;)

Gong, Qian; Dai, Chun-Yan; Zhang, Xiaohan; Wang, Xiaoli; Huang, Zhen; Xu, Aidong; Dong, Jun-Gang; Yu, Cheng-Yu

doi:10.1270/jsbbs.20017

Cited by 9 publications

(14 citation statements)

References 18 publications

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“…In untreated plants GST activity was stable and didn't vary significantly, however some plants had increased glutathione level. (1)(2)(3)(4)(5)(6) and in the lab (7)(8)(9)(10)(11)(12) are highlighted in green and purple, respectively. GSH:GSSG level in most of the susceptible plants was lower than in resistant plants, which is a sign of heavier stress.…”

Section: Resultsmentioning

confidence: 99%

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Glutathione Is Involved in Resistance of Oilseed Rape to Powdery Mildew

Mikhaylova¹,

Shein²,

Alekseev³

2020

The 1st International Electronic Conference on Plant Science

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Oilseed rape (Brassica napus) is an economically important crop. In temperate climate powdery mildew Erysiphe crucifertaum can reduce its yields dra0matically. Nevertheless, cultivars resistant to this fungal disease have not been selected yet. Plants, grown in the field and in the light chamber, were infected with powdery mildew. After 30 days there were severe signs of infection on most of the plants, however several individuals were less susceptible to it. Leaves of B. napus were used to measure the level of GSH, GSSG and GST by spectrophotometric assay. The level of total glutathione was higher in plants with increased resistant to powdery mildew than in both severely infected plants and control plants that were not subjected to the infection. The GST activity in resistant and control plants was at the same level, however in susceptible plants it was lower. Therefore, oilseed rape with naturally increased glutathione content can be used in the selection of resistant cultivars. GST and GSH genes should be also considered as targets for genetic engineering and genome editing.

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Section: Resultsmentioning

confidence: 99%

“…Infection can reduce the yield of rapeseed and its quality, causing chlorosis, necrosis and dehydration. Resistance to this pathogen is very rare in B. napus and was only achieved by hybridization with distant relatives [3]. Commercial cultivars resistant to this fungal disease have not been selected yet.…”

Section: Introductionmentioning

confidence: 99%

Glutathione Is Involved in Resistance of Oilseed Rape to Powdery Mildew

Mikhaylova¹,

Shein²,

Alekseev³

2020

The 1st International Electronic Conference on Plant Science

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“…Powdery mildew (PM) fungi are biotrophic pathogens that grow superficially on living host plants, coating the leaves, stems and fruits with spores and mycelia [ 1 , 2 ]. Cruciferous plant-specific PM disease caused by the pathogen Erysiphe cruciferarum is expanding year by year in various Brassica plants, especially rapeseed ( Brassica napus L.), which is the second-largest oilseed crop in the world as well as a vegetable plant in East Asia.…”

Section: Introductionmentioning

confidence: 99%

“…Cruciferous plant-specific PM disease caused by the pathogen Erysiphe cruciferarum is expanding year by year in various Brassica plants, especially rapeseed ( Brassica napus L.), which is the second-largest oilseed crop in the world as well as a vegetable plant in East Asia. PM is the fourth most important disease causing heavy losses to rapeseed and is widely distributed over more than 25 countries around the world [ 1 , 2 ]. Growing disease-resistant varieties is an environmentally friendly and cost-effective strategy to control PM disease.…”

Section: Introductionmentioning

confidence: 99%

Microscopic and Transcriptomic Comparison of Powdery Mildew Resistance in the Progenies of Brassica carinata × B. napus

Zhang

Gong

et al. 2022

IJMS

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Powdery mildew is a widespread disease in rapeseed due to a lack of resistant germplasm. We compared the foliar epidermal features and transcriptomic responses between the resistant (R) and susceptible (S) plants among the two parents and progenies of Brassica carinata × B. napus. The amount of cuticular wax and callose deposition on the R plants was much lower than that on the S plants; hence, these chemicals are not all essential to pre-penetration resistance, although the cuticular wax on the R plants had more needle-like crystals. A total of 1049 genes involved in various defense responses were expressed differentially among the R/S plants. The expression levels of two well-known susceptibility genes, MLO6 and MLO12, were much lower in the R plant, indicating an important role in PM resistance. A set of genes related to wax biosynthesis (KCS6, LACS2, CER and MAH1), cell wall modification (PMR5, PMEI9, RWA2, PDCB1 and C/VIF2), chloroplast function (Chlorophyllase-1, OEP161, PSBO1, CP29B and CSP41b), receptor kinase activity (ERECTA, BAK1, BAM2, LYM1, LYM3, RLK902, RLP11, ERL1 and ERL2), IPCS2, GF14 lambda, RPS4 and RPS6 were highly expressed in the R plants. In the S plants, most highly expressed genes were involved in later defense responses, including CERK1, LYK4, LIK1, NIMIN-1, CHITINASE 10, PECTINESTERASE, CYP81F2 and RBOHF and the genes involved in salicylic acid-dependent systemic acquired resistance and hypersensitive responses, indicating the occurrence of severe fungal infection. The results indicate that some uncertain pre-penetration defenses are pivotal for high resistance, while post-penetration defenses are more important for the S plant survival.

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“…Powdery mildew can reduce the yield and quality of rapeseed seeds, causing chlorosis, necrosis and dehydration. Resistance to this pathogen in B. napus is very rare, and was achieved only by hybridization with distant relatives [6]. Resistant commercial cultivars have not yet been selected.…”

Section: Introductionmentioning

confidence: 99%

The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress

Mikhaylova

Khusnutdinov

Shein

et al. 2021

Plants

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Oilseed rape (Brassica napus) is an economically important crop. In a temperate climate, powdery mildew Erysiphe crucifertaum can drastically reduce its yield. Nevertheless, cultivars resistant to this fungal disease have not yet been selected. Glutathione S-transferase GSTF11 is involved in glucosinolate (GSL) biosynthesis and response to stress, including fungal deceases. However, the impact of exogenous GSTF11 gene expression on resistance to powdery mildew has not yet been confirmed and requires further investigation. Transgenic B. napus was generated for this purpose. It demonstrated increased GST activity and a higher GSH:GSSG ratio under normal conditions. Powdery mildew Erysiphe crucifertaum caused 50% mortality in wild type (WT) plants. In most of transgenic plants, mycelium growth was inhibited. The infection contributed to higher GSTF11 expression and increased levels of glutathione (GSH) and oxidized glutathione (GSSG) in both transgenic and WT plants. In contrast, GSTF11 mRNA content, GST activity and GSSG level were lower only in WT plants. In transgenic plants, increased resistance to powdery mildew correlated with a lower GSH:GSSG ratio, indicating a higher content of neutralized toxic molecules. GSTF11 expression was also affected by cold stress, but not drought. At −1 °C, the expression level increased only in transgenic plants. Therefore, GSTF11 appears to be nonspecific and is able to protect plants under several types of stress. This gene could be used as a target in the production of stress tolerant cultivars.

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Towards breeding of rapeseed (<i>Brassica napus</i>) with alien cytoplasm and powdery mildew resistance from Ethiopian mustard (<i>Brassica carinata</i>)

Cited by 9 publications

References 18 publications

Glutathione Is Involved in Resistance of Oilseed Rape to Powdery Mildew

Glutathione Is Involved in Resistance of Oilseed Rape to Powdery Mildew

Microscopic and Transcriptomic Comparison of Powdery Mildew Resistance in the Progenies of Brassica carinata × B. napus

The Role of the GSTF11 Gene in Resistance to Powdery Mildew Infection and Cold Stress

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