Towards bioinspired<i>in vitro</i>models of intestinal mucus

Sardelli, Lorenzo; Pacheco, Daniela Peneda; Ziccarelli, Anna; Tunesi, Marta; Caspani, Omar; Fusari, Andrea; Briatico‐Vangosa, Francesco; Giordano, Carmen; Petrini, Paola

doi:10.1039/c9ra02368b

Cited by 41 publications

(39 citation statements)

References 96 publications

(112 reference statements)

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“…but it is also strongly dependent on the microbiota-host crosstalk itself (Sardelli et al, 2019). For this reason, gut-on-a-chip systems evolved to coculture bacteria into the luminal channel, in direct contact with the mucus of intestine epithelial layer, while keeping the microenvironment perfused (Kim et al, 2012;Shin et al, 2019;Sontheimer-Phelps et al, 2020).…”

Section: Advanced Culture Systems To Study Microbiota-host Interactionmentioning

confidence: 99%

Technological tools and strategies for culturing human gut microbiota in engineered in vitro models

Sardelli

Perottoni

Tunesi

et al. 2021

Biotech & Bioengineering

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The gut microbiota directly impacts the pathophysiology of different human body districts. Consequently, microbiota investigation is an hot topic of research and its in vitro culture has gained extreme interest in different fields. However, the high sensitivity of microbiota to external stimuli, such as sampling procedure, and the physicochemical complexity of the gut environment make its in vitro culture a challenging task. New engineered microfluidic gut-on-a-chip devices have the potential to model some important features of the intestinal structure, but they are usually unable to sustain culture of microbiota over an extended period of time. The integration of gut-on-a-chip devices with bioreactors for continuous bacterial culture would lead to fast advances in the study of microbiota-host crosstalk. In this review, we summarize the main technologies for the continuous culture of microbiota as upstream systems to be coupled with microfluidic devices to study bacteriahost cells communication. The engineering of integrated microfluidic platforms, capable of sustaining both anaerobic and aerobic cultures, would be the starting point to unveil complex biological phenomena proper of the microbiota-host crosstalks, paving to way to multiple research and technological applications.

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Section: Advanced Culture Systems To Study Microbiota-host Interactionmentioning

confidence: 99%

Technological tools and strategies for culturing human gut microbiota in engineered in vitro models

Sardelli

Perottoni

Tunesi

et al. 2021

Biotech & Bioengineering

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“…After its contact with gastric and intestinal fluid, the nanocarrier has to diffuse through mucus to be absorbed or at least to release its cargo in the mucus in the vicinity of drug absorption sites. After the stability issue, the diffusion within mucus has been raised as an important barrier for nanocarrier absorption and barrier properties of mucus have been fully reviewed elsewhere [25][26][27].…”

Section: Mucus Permeation Of Np As a Critical Barrier For Absorptionmentioning

confidence: 99%

Specificity of pharmacokinetic modeling of nanomedicines

Lebreton

Legeay

Saulnier

et al. 2021

Drug Discovery Today

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“…Moreover, the flow of fluids does not reproduce the peristaltic movements of the intestine, which are fundamental for the bacteria clearance in the gut and adsorption of molecules in vivo . Although this system is suitable for the study of the behavior of the gut microbiota, it does not take into account the presence of mucus which is the complex physiological environment where bacteria live and diffusion of substances is regulated (Sardelli et al, 2019 ). In order to improve the SHIME to overcome this last limitation, the same group optimized the system including the mucosal component in the colon compartments (M-SHIME).…”

Section: Experimental Models To Address the Role Of Gut Microbiota Inmentioning

confidence: 99%

Human Gut-Microbiota Interaction in Neurodegenerative Disorders and Current Engineered Tools for Its Modeling

Ceppa

Izzo

Sardelli

et al. 2020

Front. Cell. Infect. Microbiol.

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The steady increase in life-expectancy of world population, coupled to many genetic and environmental factors (for instance, pre- and post-natal exposures to environmental neurotoxins), predispose to the onset of neurodegenerative diseases, whose prevalence is expected to increase dramatically in the next years. Recent studies have proposed links between the gut microbiota and neurodegenerative disorders such as Alzheimer's and Parkinson's diseases. Human body is a complex structure where bacterial and human cells are almost equal in numbers, and most microbes are metabolically active in the gut, where they potentially influence other target organs, including the brain. The role of gut microbiota in the development and pathophysiology of the human brain is an area of growing interest for the scientific community. Several microbial-derived neurochemicals involved in the gut-microbiota-brain crosstalk seem implicated in the biological and physiological basis of neurodevelopment and neurodegeneration. Evidence supporting these connections has come from model systems, but there are still unsolved issues due to several limitations of available research tools. New technologies are recently born to help understanding the causative role of gut microbes in neurodegeneration. This review aims to make an overview of recent advances in the study of the microbiota-gut-brain axis in the field of neurodegenerative disorders by: (a) identifying specific microbial pathological signaling pathways; (b) characterizing new, advanced engineered tools to study the interactions between human cells and gut bacteria.

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Towards bioinspiredin vitromodels of intestinal mucus

Abstract: Intestinal mucus is an anisotropic biological structure that acts as a barrier between the external environment and the epithelium.

Cited by 41 publications

References 96 publications

Technological tools and strategies for culturing human gut microbiota in engineered in vitro models

Technological tools and strategies for culturing human gut microbiota in engineered in vitro models

Specificity of pharmacokinetic modeling of nanomedicines

Human Gut-Microbiota Interaction in Neurodegenerative Disorders and Current Engineered Tools for Its Modeling

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