Towards bio-compatible magnetic nanoparticles: Immune-related effects, in-vitro internalization, and in-vivo bio-distribution of zwitterionic ferrite nanoparticles with unexpected renal clearance

Ferretti, Anna M.; Usseglio, Sandro; Mondini, Sara; Drago, Carmelo; Mattina, Rosa La; Chini, Bice; Verderio, Claudia; Leonzino, Marianna; Cagnoli, Cinzia; Joshi, Pooja; Boraschi, Diana; Italiani, Paola; Li, Yang; Swartzwelter, Benjamin J; Sironi, Luigi; Castiglioni, Laura; Guerrini, Uliano; Ponti, Alessandro

doi:10.1016/j.jcis.2020.08.026

Cited by 29 publications

(25 citation statements)

References 88 publications

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“…Primary Response of Human Monocytes to NP Exposure NP aggregation during in vitro assays is a common disturbing factor when studying NP-cell interactions. In our case, aggregation is a very minor factor since (i) both AuNP types are colloidally stabilized by the pre-treatment with human serum (likely due to protein adsorption), and (ii) both FeOxNP types are coated with ZDS, which is known to endow high colloidal stability (42). Therefore, we hold that NP aggregation is of minor importance in the present study, in particular at the selected concentrations.…”

Section: Selection Of the Np Concentrations For Biological Studiesmentioning

confidence: 74%

“…Details of the synthesis and characterisation of FeOxNP coated with zwitterionic dopamine sulfonate (ZDS) have been published elsewhere (42). For all NP types, the main physico-chemical characteristics are reported in Figure 1 and Tables 1, 2.…”

Section: Nanoparticle Synthesis and Characterisationmentioning

confidence: 99%

“…The citrate anions are weakly bound (physisorbed) to the AuNPs, and are thus not able to prevent aggregation in RPMI-1640 medium, probably because they are displaced by the non-salt components in the culture medium. Conversely, the zwitterionic sulfobetaine ZDS is strongly bonded to the FeOxNP and is able to protect NP from aggregation and protein adsorption (42), thanks to its hydrophilicity and overall neutrality. The pre-treatment with human serum afforded colloidal stability in culture medium for spherical AuNP and, up to a concentration of 1.4 µg/mL, also for rod-shaped AuNP.…”

Section: Physico-chemical Characterisation Of the Np Used In This Studymentioning

confidence: 99%

“…Several studies report the capacity of FeOxNP to induce the production of inflammatory/toxic reactive oxygen species [reviewed in (35,38)]. Other studies, however, do not find inflammation-related effects on macrophages, dendritic cells and microglial cells in vitro (36,(39)(40)(41)(42). While AuNP are generally considered biocompatible, variable results have been obtained, depending on the experimental model/cell type used, the dose, size and shape of the NP (43)(44)(45)(46).…”

Section: Introductionmentioning

confidence: 99%

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Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes

et al. 2021

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Engineered nanoparticles used for medical purposes must meet stringent safety criteria, which include immunosafety, i.e., the inability to activate possibly detrimental immune/inflammatory effects. Even medical nanomaterials devoid of direct immunotoxic or inflammatory effects may have an impact on human health if able to modify innate memory, which is the ability to “prime” future immune responses towards a different, possibly more detrimental reactivity. Although innate memory is usually protective, anomalous innate memory responses may be at the basis of immune pathologies. In this study, we have examined the ability of two nanomaterials commonly used for diagnostic imaging purposes, gold and iron oxide nanoparticles, to induce or modulate innate memory, using an in vitro model based on human primary monocytes. Monocytes were exposed in culture to nanoparticles alone or together with the bacterial agent LPS (priming phase/primary response), then rested for six days (extinction phase), and eventually challenged with LPS (memory/secondary response). The memory response to the LPS challenge was measured as changes in the production of inflammatory (TNFα, IL-6) and anti-inflammatory cytokines (IL-10, IL-1Ra), as compared to unprimed monocytes. The results show that both types of nanoparticles can have an effect in the induction of memory, with changes observed in the cytokine production. By comparing nanomaterials of different shapes (spherical vs. rod-shaped gold particles) and different size (17 vs. 22 nm diameter spherical iron oxide particles), it was evident that innate memory could be differentially induced and modulated depending on size, shape and chemical composition. However, the main finding was that the innate memory effect of the particles was strongly donor-dependent, with monocytes from each donor showing a distinct memory profile upon priming with the same particles, thereby making impossible to draw general conclusions on the particle effects. Thus, in order to predict the effect of imaging nanoparticles on the innate memory of patients, a personalised profiling would be required, able to take in consideration the peculiarities of the individual innate immune reactivity.

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Section: Selection Of the Np Concentrations For Biological Studiesmentioning

confidence: 74%

Section: Nanoparticle Synthesis and Characterisationmentioning

confidence: 99%

Section: Physico-chemical Characterisation Of the Np Used In This Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes

et al. 2021

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“…Exploiting magnetite NPs of different size to catalyze the polymerization of AD and prepare PANI/Fe3O4 nanocomposites would allow one to tune the magnetic properties of the composites provided that the NPs are size monodisperse and effective as a catalyst. The first requirement can be met thanks to the well-established methods of size-controlled colloidal nanochemistry (Ferretti et al, 2021). As to the second one, the activity of a heterogeneous catalysts is strongly related to its particle size since it is intrinsically dependent on the surfaceto-volume ratio, which dramatically grows passing from bulk to nano-dimensions.…”

Section: Introductionmentioning

confidence: 99%

Size-dependent catalytic effect of magnetite nanoparticles in the synthesis of tunable magnetic polyaniline nanocomposites

Falletta

Ferretti²,

Mondini³

et al. 2021

Chem. Pap.

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Nanocomposites comprising magnetic nanoparticles (NPs) embedded in an organic conducting polymer are promising materials that may allow one to exploit synergic effects between the electrically conducting and the magnetically permeable components. Having already shown that magnetite NPs can be conveniently used as a catalyst for the oxidative polymerization of the aniline dimer resulting in NPs embedded in the final composite and how to modulate the magnetic coercivity of the composites, we now turn to investigate how the size of magnetite NPs affects the polymerization and the properties of the final composite. Magnetite NPs of diameter 2.3, 10, and 27 nm turned out to be effective catalysts with cheap oxidants such as H2O2 and O2. Yield data show that the rate-determining step occurs on the NP surface.Extensive characterization shows that the NPs are well-dispersed in the composite with no significant morphological change. The static magnetic properties of the composites are widely different, e. g, the magnetic blocking temperature shifts from 290 K for 27 nm NPs to 54 K for 10 nm NPs while composites with 2.3 nm NPs are virtually unblocked down to 5 K. The dynamic electromagnetic behavior studied up to the microwave range only shows energy absorptions associated to the ferromagnetic resonance, at frequencies around 1 GHz.

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Highly Sensitive Early Diagnosis of Kidney Damage Using Renal Clearable Zwitterion‐Coated Ferrite Nanoprobe via Magnetic Resonance Imaging In Vivo

Zhou,

Dong,

Wang

et al. 2024

Adv Healthcare Materials

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Iron oxide nanoprobes exhibit substantial potential in magnetic resonance imaging (MRI) of kidney diseases and can eliminate the nephrotoxicity of gadolinium‐based contrast agents (GBCAs). Nevertheless, there is an extreme shortage of highly sensitive and renal clearable iron oxide nanoprobes suitable for early kidney damage detection through MRI. Herein, we propose a renal clearable ultra‐small ferrite nanoprobe (UMFNPs@ZDS) for highly sensitive early diagnosis of kidney damage via structural and functional MRI in vivo for the first time. The nanoprobe comprises a ferrite core coated with a zwitterionic layer, and possesses a high T1 relaxivity (12.52 mM−1s−1), a small hydrodynamic size (6.43 nm), remarkable water solubility, excellent biocompatibility, and impressive renal clearable ability. In a rat model of unilateral ureteral obstruction (UUO), the nanoprobe‐based MRI can not only accurately visualize the locations of renal injury, but also provide comprehensive functional data including peak value, peak time, relative renal function (RRF), and clearance percentage via MRI. Our findings prove the immense potential of ferrite nanoprobes as a superior alternative to GBCAs for the early diagnosis of kidney damage.This article is protected by copyright. All rights reserved

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Towards bio-compatible magnetic nanoparticles: Immune-related effects, in-vitro internalization, and in-vivo bio-distribution of zwitterionic ferrite nanoparticles with unexpected renal clearance

Cited by 29 publications

References 88 publications

Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes

Personalised Profiling of Innate Immune Memory Induced by Nano-Imaging Particles in Human Monocytes

Size-dependent catalytic effect of magnetite nanoparticles in the synthesis of tunable magnetic polyaniline nanocomposites

Highly Sensitive Early Diagnosis of Kidney Damage Using Renal Clearable Zwitterion‐Coated Ferrite Nanoprobe via Magnetic Resonance Imaging In Vivo

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