Towards Binding Mechanism of Cu2+ on Creatine Kinase from Pelodiscus sinensis: Molecular Dynamics Simulation Integrating Inhibition Kinetics Study

Cai, Yan; Lee, Jinhyuk; Wang, Wei; Park, Yong‐Doo; Qian, Guo‐Ying

doi:10.2174/0929866524666170227122706

Cited by 4 publications

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“…Previous reports have discussed the biochemical characteristics of PSCK in P. sinensis, mainly focusing on the muscle (PSCK-M) and brain (PSCK-B) types in vitro folding studies [15,16,20,21,29,30]. In this study, we have focused on the mitochondrial type such as PSCK-S and made a comparison between three types of PSCK isozymes.…”

Section: Discussionmentioning

confidence: 99%

“…The supernatant was obtained by freezing centrifugation at 5000 rpm for 15 min for biochemical parameters' assay. The total activity of PSCK from the tissues was measured following proton generation during the reaction of ATP and creatine with thymol blue at 597 nm and 25°C, as previously described [15,16,29,30]. The activities of total superoxide dismutase (T-SOD), lactate dehydrogenase assay (LDH), malondialdehyde (MDA), catalase (CAT), and the content of ATP were measured according to the kit manuals (A020-2 for LDH, A001-1 for T-SOD, A007-1 for CAT, A003-2 for MDA, and A095 for ATP) provided by Nanjing Jiancheng Biochemical Reagent Co. Ltd. (Nanjing, China) using the clinical biochemical indicator autoanalyzer: Infinite M200Pro spectrometer (Tecan, Switzerland), according to the manufacturer's instructions.…”

Section: Determination Of Biochemical Enzyme Activitiesmentioning

confidence: 99%

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Comparative studies of the expression of creatine kinase isoforms under immune stress in Pelodiscus sinensis

Wang

Lee

et al. 2020

International Journal of Biological Macromolecules

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The expression and localization of different isoforms of creatine kinase in Pelodiscus sinensis (PSCK) were studied to reveal the role of PSCK isozymes (PSCK-B, PSCK-M, PSCK-S) under bacterial infection-induced immunologic stress. The computational molecular dynamics simulations predicted that PSCK-S would mostly possess a kinase function in a structural aspect when compared to PSCK-B and PSCK-M. The assay of biochemical parameters such as total superoxide dismutase (T-SOD), lactate dehydrogenase (LDH), malondialdehyde (MDA), catalase (CAT), and the content of ATP were measured along with total PSCK activity in different tissue samples under bacterial infection. The expression detections of PSCK isozymes in vitro and in vivo were overall well-matched where PSCK isozymes were expressed differently in P. sinensis tissues. The results showed that PSCK-B mostly contributes to the spleen, followed by the liver and myocardium; PSCK-M mostly contributes to the liver, followed by the myocardium and skeletal muscle, while PSCK-S contributes to the spleen and is uniquely expressed in skeletal muscle. Our study suggests that the various alterations of PSCK isozymes in tissues of P. sinensis are prone to defense the bacterial infection and blocking energetic imbalance before severe pathogenesis turned on in P. sinensis.

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