Towards an on-chip platform for the controlled application of forces via magnetic particles: A novel device for mechanobiology

Monticelli, Marco; Albisetti, Edoardo; Petti, Daniela; Conca, Dario Valter; Falcone, Matteo; Sharma, Parikshit Pratim; Bertacco, Riccardo

doi:10.1063/1.4917191

Cited by 5 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, magnetic tweezers and micromagnetophoretic patterns offer significant advancements by overcoming the abovementioned complications toward precise particle, droplet, and cell manipulations. Furthermore, we have demonstrated a class of integrated circuits of micromagnetophoretic patterns, such as conductors, diodes, capacitors, and transistors to execute sequential and parallel operations on an ensemble of single particles and cells .…”

Section: Multifarious Transit Gating Of Particles Moving Along the Rementioning

confidence: 99%

Multifarious Transit Gates for Programmable Delivery of Bio‐functionalized Matters

Torati

Kim

et al. 2019

Small

View full text Add to dashboard Cite

Programmable delivery of biological matter is indispensable for the massive arrays of individual objects in biochemical and biomedical applications. Although a digital manipulation of single cells has been implemented by the integrated circuits of micromagnetophoretic patterns with current wires, the complex fabrication process and multiple current operation steps restrict its practical application for biomolecule arrays. Here, a convenient approach using multifarious transit gates is proposed, for digital manipulation of biofunctionalized microrobotic particles that can pass through the local energy barriers by a time‐dependent pulsed magnetic field instead of multiple current wires. The multifarious transit gates including return, delay, and resistance linear gates, as well as dividing, reversed, and rectifying T‐junction gates, are investigated theoretically and experimentally for the programmable manipulation of microrobotic particles. The results demonstrate that, a suitable angle of the gating field at a suitable time zone is crucial to implement digital operations at integrated multifarious transit gates along bifurcation paths to trap microrobotic particles in specific apartments, paving the way for flexible on‐chip arrays of biomolecules and cells.

show abstract

Section: Multifarious Transit Gating Of Particles Moving Along the Rementioning

confidence: 99%

Multifarious Transit Gates for Programmable Delivery of Bio‐functionalized Matters

Torati

Kim

et al. 2019

Small

View full text Add to dashboard Cite

show abstract

“…The viscous friction on particles in a fluid is described by the Stokes equation (see Supplementary Information for details). For a typical manipulation velocity v= 10 µm/s, it is equal to 85 fN and can be neglected if compared to F m , which is in the order of hundreds pN 41,49 . Even gravity and buoyancy forces are much less intense (≈ 10 fN) than F m .…”

Section: Simulations Of Magnetic Forces Exerted On Cellsmentioning

confidence: 99%

“…Crucial for applications, in ref. 49 some of the authors demonstrated that the magnetic force can be tuned varying the intensity of H e, which affects the beads magnetization and consequently F m (see the first term in Equation 1). Our platform thus provides an efficient method for applying localized and tuneable magnetic forces to the cell, whose intensity can be quantitatively evaluated and correlated to the observation of confocal microscopy.…”

Section: Simulations Of Magnetic Forces Exerted On Cellsmentioning

confidence: 99%

Magnetic domain wall tweezers: a new tool for mechanobiology studies on individual target cells

et al. 2016

Self Cite

View full text Add to dashboard Cite

In vitro tests are of fundamental importance for investigating cell mechanisms in response to mechanical stimuli or the impact of the genotype on cell mechanical properties. In particular, the application of controlled forces to activate specific bio-pathways and investigate their effects, mimicking the role of the cellular environment, is becoming a prominent approach in the emerging field of mechanobiology. Here, we present an on-chip device based on magnetic domain wall manipulators, which allows the application of finely controlled and localized forces on target living cells. In particular, we demonstrate the application of a magnetic force in the order of hundreds of pN on the membrane of HeLa cells cultured on-chip, via manipulation of 1 μm superparamagnetic beads. Such a mechanical stimulus produces a sizable local indentation of the cellular membrane of about 2 μm. Upon evaluation of the beads' position within the magnetic field originated by the domain wall, the force applied during the experiments is accurately quantified via micromagnetic simulations. The obtained value is in good agreement with that calculated by the application of an elastic model to the cellular membrane.

show abstract

“…The individual particle manipulation, on the contrary, allows 1D, 2D, and 3D positioning, as well as possible orientation control of an individual magnetic microparticle. 1D transportation has been carried out by serpentine microcurrent lines or by zig‐zag micropatterned domain walls . Electromagnetic needles have also been used for 1D magnetophoretic attraction and 2D guiding of superparamagnetic microparticles, as well as DNA stretching.…”

Section: Introductionmentioning

confidence: 99%

“…Other, 2D transportation with potential rotation of microparticles has been achieved by micropatterned thin film magnetic elements and current wires, two‐pair electromagnets, as well as magnetooptical tweezers . The systems using micropatterned elements are usually coupled with an external magnetic field coming from a permanent magnet, single, or two pair Helmholtz coils or electromagnets . In addition, multipole solenoids enable 3D positioning…”

Section: Introductionmentioning

confidence: 99%

Manipulating Superparamagnetic Microparticles with an Electromagnetic Needle

Cenev

Zhang

Sariola

et al. 2017

Adv Materials Technologies

View full text Add to dashboard Cite

organisms. The manipulation of superparamagnetic microparticles, in particular, has a wide range of applications, including magnetic logic; [13] sorting and trapping of magnetically tagged proteins, [14] cells, [15] and microorganisms; [16] microfluidic mixing; [17] magnetoresistive biosensing; [18] immunoassays; [19] and lab-on-a-tube devices. [20] In principle, the manipulation methods of these microparticles can be cate gorized into two groups: batch and individual particle manipulation. In batch manipulation, the magnetic field and field gradient originate from sources, such as external permanent magnet, [19] micropatterned current wires, [21,22] and microfabricated arrays of permanent magnets. [23] These systems are usually coupled with fluidic flows within fluidic chips. The microparticles are treated collectively as a lot with the manipulation strategies being mainly limited to trapping, separation (e.g., magnetic from nonmagnetic particles) or unidirectional transportation. [24] The individual particle manipulation, on the contrary, allows 1D, 2D, and 3D positioning, as well as possible orientation control of an individual magnetic microparticle. 1D transportation has been carried out by serpentine microcurrent lines [25] or by zig-zag Selective, precise, and high-throughput manipulation of individual superparamagnetic microparticles has profound applications in performing locationtailored in vitro biomedical studies. The current techniques for manipulation of microparticles allow only a single particle in the manipulation workspace, or simultaneous transportation of multiple microparticles in batches. In this work, a method based on a robotized electromagnetic needle for manipulation of individual superparamagnetic microparticles within a microparticle population is introduced. By automatically controlling the highly localized magnetic field of the needle, a single microparticle is selectively picked when its neighboring particle is few micrometers away. Supported by the nanometer resolution of the robotic positioner, particles are placed at sub-micrometer precision. This manipulation technique allows the creating of arbitrary patterns, sorting of microparticles based on size and morphology, and transporting of individual microparticles in 3D space. Therefore, this approach has the potential to enable more deterministic and quantitative microanalysis and microsynthesis using superparamagnetic microparticles.

show abstract

Towards an on-chip platform for the controlled application of forces via magnetic particles: A novel device for mechanobiology

Cited by 5 publications

References 19 publications

Multifarious Transit Gates for Programmable Delivery of Bio‐functionalized Matters

Multifarious Transit Gates for Programmable Delivery of Bio‐functionalized Matters

Magnetic domain wall tweezers: a new tool for mechanobiology studies on individual target cells

Manipulating Superparamagnetic Microparticles with an Electromagnetic Needle

Contact Info

Product

Resources

About