Towards an efficient phenotypic classification of fungal cultures from environmental samples using digital imagery

Pietrowski, Andrea; Flessa, Fabienne; Rambold, Gerhard

doi:10.1007/s11557-011-0753-2

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…Extented networks of melanized mycelium of A. cytisporea can be observed in and on dead leaf tissue (Figure 2a). Interestingly, Pietrowski, Flessa, and Rambold (2012) isolated an Allantophomopsis species as an endophyte from C. vulgaris leaves that possesses an ITS identical to that of A. cytisporea strain A_AR_1_06 isolated by Schneider et al (2009) from an epiphytic mycelial mat on J. communis subsp. nana reminiscent of H. pinetorum (Table 2, Figure 3).…”

Section: Discussionmentioning

confidence: 99%

The endophyte Allantophomopsis cytisporea is associated with snow blight on Calluna vulgaris in the Alps—An effect of climate change?

Sieber

2019

Arctic, Antarctic, and Alpine Research

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Shoots of Calluna vulgaris, Erica carnea, Juniperus communis subsp. nana, Picea abies, and Pinus mugo subsp. mugo covered with felty, melanized epiphytic mycelia typical for brown felt blight caused by Herpotrichia pinetorum were collected at several locations in the Swiss Alps. Most cultures prepared from the mycelia on J. communis subsp. nana and P. abies were H. pinetorum, whereas the majority of cultures from P. mugo subsp. mugo and C. vulgaris were identified by internal transcribed spacer ITS1-5.8S-ITS2 sequencing and morphology as Allantophomopsis cytisporea. The fungus tolerates low temperatures, has an optimum between 16°C and 24°C, and ceases to grow at 28°C. 35°C is lethal. A. cytisporea is known as the causal agent of cranberry black rot on Vaccinium macrocarpon but has never been described as a snow mold. A. cytisporea is an endophyte in C. vulgaris but seems able to kill leaflets and whole shoots during winter. The epiphytic mycelium can expand from C. vulgaris to neighboring shoots of P. mugo subsp. mugo and J. communis subsp. nana below the snow where it forms epiphytic mycelial mats reminiscent of H. pinetorum. H. pinetorum has a strong antibiotic effect against A. cytisporea at 4°C and 20°C, whereas A. cytisporea grows faster at these temperatures. The effects of climate change on the interaction between the two snow mold fungi and their consequences on regeneration of woody plants at timberline are discussed.

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Section: Discussionmentioning

confidence: 99%

The endophyte Allantophomopsis cytisporea is associated with snow blight on Calluna vulgaris in the Alps—An effect of climate change?

Sieber

2019

Arctic, Antarctic, and Alpine Research

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“…A colour chart (RAL 7005-HR mousy; German Institute for Quality Assurance and Certification e. V.) was used as the standard background. Settings: lens aperture of 1/16, sensitivity ISO 100, balanced preset d-0, colour mode III (Adobe RGB), and shutter speed of 0.5 s (Pietrowski et al 2010). The CMYK values of the digital images for testing the degree of pigmentation were classified as 'dark': C: 39-73%; M: 50-75%; Y: 51-94%; K: 21-83%.…”

Section: Cultivation and Pigmentation Testmentioning

confidence: 99%

Comparative analyses of sooty mould communities from Brazil and Central Europe

et al. 2021

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To gain an insight into fungal sooty mould communities on leaves of trees and shrubs in the tropics and in temperate regions, 47 biofilms of the Mata Atlântica rainforest relic and the Caatinga vegetation in the state of Sergipe, Northeast Brazil, and from Central European colline and alpine zones were compared. The four sampling sites clearly differed in composition of their epiphyllous fungal communities. The fungal OTUs from all sites belonged mainly to the Ascomycota, with Dothideomycetes being the dominant class. The core community group consisted of a few site-specific representatives in co-occurrence with the ubiquitous Mycosphaerella tassiana and Aureobasidium pullulans. Most species of the core community were dark pigmented and were accompanied by facultative unpigmented or lightly pigmented species. Among the cultivable fungal species, the proportion of melanised species was significantly more abundant in samples from the two European sites, which supports the theory of thermal melanism. The identity of the host plant had a stronger impact on fungal community composition than the presence of sap-feeding insects.

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“…fruits by forming highly branched, well-differentiated hyphae than the other crops (Liao et al, 2011). Moreover, many studies suggest extracting the colors, shapes, and textures from the images of plant segments that are infected and cultured fungal strain samples (Pietrowski et al, 2012;Singh & Chetia, 2017). Their experiments were implemented by nondestructive and exact identification methods based on digital images of spectral sensors (Barbedo, 2013;Pujari et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

New method for detecting Collectorichum species found in Korea using image analysis

Baek

Kim

et al. 2022

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Colletotrichum acutatum spp. infects various economical crops worldwide and causes massive loss on their yields. Among those, Capsicum spp., which known as chili pepper, is on a critical issue by those pathogens. Due to the lack of their genetic markers in Korea, the unidentifiable various species of C. acutatum obstructs the mechanism studies of these pathogens and the selection of disease resistant breed lines. Therefore, we screened RGB images of the colonization progresses of pathogens to identify the species of Ca40042, K1, NN, AS2, and SW1 by time and temperature. Cultivated pathogens such as Ca40042, K1, and SW1 were detectable on quantified shape and color data of images from specific temperature conditions, while other pathogens were difficult to recognize. Although several limitations exist in identification results of current experiment, but also, we can expect this method can suggest the possibility to replace the genetic marker methods which is now unavailable in Korea.

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Towards an efficient phenotypic classification of fungal cultures from environmental samples using digital imagery

Cited by 3 publications

References 25 publications

The endophyte Allantophomopsis cytisporea is associated with snow blight on Calluna vulgaris in the Alps—An effect of climate change?

The endophyte Allantophomopsis cytisporea is associated with snow blight on Calluna vulgaris in the Alps—An effect of climate change?

Comparative analyses of sooty mould communities from Brazil and Central Europe

New method for detecting Collectorichum species found in Korea using image analysis

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