Towards a synthetic view of potato cold and salt stress response by transcriptomic and proteomic analyses

Abstract

Potato can suffer from several abiotic stresses such as cold temperature, high soil salinity, lack of water or heavy metal exposure, to name a few. They are known to affect plant growth as well as productivity, with differential regulations at several levels. Potato response to cold and salt exposure was investigated at both transcriptomic and proteomic levels in a growth chamber experiment. Cold exposure in potato resulted in a higher number of significantly differentially regulated genes compared to salt exposure, whereas there were nearly three times more differentially regulated proteins after salt exposure when compared to cold exposure. The allocation of up and down-regulated genes at the functional category level also differed between salt and cold exposure although common trends, previously described in various abiotic stresses, were observed. In both stresses, the majority of photosynthesis-related genes were down-regulated whereas cell rescue and transcription factor-related genes were mostly up-regulated. In the other functional categories no common trend was observed; salt exposure results displayed a strong down-regulation of genes implicated in primary metabolism, detoxication apparatus and signal transduction, whereas upon cold exposure, up and down-regulated genes were similar in number. At the proteomic level, the abundance of the majority of identified proteins was increased except for the photosynthesis-related proteins, which were mostly less abundant after both salt and cold exposure. Common responses between salt and cold stress and specific responses inherent to these abiotic stresses are described.

Differential Protein Expression in Response to Abiotic Stress in Two Potato Species: Solanum commersonii Dun and Solanum tuberosum L.

“…However, several studies have shown that changes in mRNA transcript levels do not automatically imply corresponding changes in protein amount or activity [14,15]. A proteomic approach complements the genomic and transcriptomic data by looking at the actual protein population of a specific tissue, cell or cellular compartment.…”

“…Several proteome studies have mainly been executed on tuber tissue [20–22] and in connection with phytopathogens [23–25]. Other studies on protein changes related to abiotic stress tolerance in potato have also been implemented until now [15,26]. …”

Plant Abiotic Stress:‘Omics’ Approach

“…Functional analyses of the result showed repression of carbohydrate anabolism and photosynthesis whereas carbohydrate catabolism was upregulated along with some proteins involved in jasmonic acid biosynthesis pathway, stress, defense and secondary metabolism [11]. [12] analyzed the effect of salt and cold stresses on the leaf transcriptome and proteome of potato which shows a large number of differentially regulated genes and proteins. It was observed that when the plant was subjected to salt stress a large number of genes associated with primary metabolism, signal transduction and detoxification apparatus were down regulated whereas when the plant was exposed to cold stress the number of genes showing up-regulation and down regulation was equal.…”

Achievements and prospects of applying high-throughput sequencing techniques to potato genetics and breeding

“…ncbi.nlm.nih.gov/geo/) -это база данных, в которой собра ны результаты высокопроизводительного секвенирования транскриптомов (RNAseq) и анализа экспрессии генов с использованием микрочипов, в том числе для S. tuberosum (Rensink et al, 2005;Carvallo et al, 2011;Evers et al, 2012;Zhang et al, 2013;Stare et al, 2015;Gálvez et al, 2016). База данных GEO включает несколько разделов, в том числе DataSets, который содержит описание эксперимен тов по секвенированию транскриптомов.…”