Towards a preventive and/or curative treatment of esca in grapevine trunk disease: General basis in the elaboration of treatments to control plant pathogen attacks

Roblin, Gabriel; Luini, Estelle; Fleurat‐Lessard, Pierrette; Larignon, Philippe; Berjeaud, Jean‐Marc

doi:10.1016/j.cropro.2018.10.016

Cited by 9 publications

(13 citation statements)

References 86 publications

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“…This would be the most practical approach considering the experience of winegrowers. According to Roblin et al [43], the compounds sprayed on the leaf blades would be able to migrate to the fungal target in the trunk or to trigger the plant defense reaction in distal parts of the plant. In fact, successful use of foliar sprays of chitosan on grapevine plants artificially infested with Phaeomoniella chlamydospora or Neonectria liriodendri have been reported in the literature [3].…”

Section: Discussionmentioning

confidence: 99%

Antifungal Agents Based on Chitosan Oligomers, ε-polylysine and Streptomyces spp. Secondary Metabolites against Three Botryosphaeriaceae Species

et al. 2019

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Grapevine trunk diseases (GTDs) are a major threat to the wine and grape industry. The aim of the study was to investigate the antifungal activity against Neofusicoccum parvum, Diplodia seriata, and Botryosphaeria dothidea of ε-polylysine, chitosan oligomers, their conjugates, Streptomyces rochei and S. lavendofoliae culture filtrates, and their binary mixtures with chitosan oligomers. In vitro mycelial growth inhibition tests suggest that the efficacy of these treatments, in particular those based on ε-polylysine and ε-polylysine:chitosan oligomers 1:1 w/w conjugate, against the three Botryosphaeriaceae species would be comparable to or higher than that of conventional synthetic fungicides. In the case of ε-polylysine, EC90 values as low as 227, 26.9, and 22.5 µg·mL−1 were obtained for N. parvum, D. seriata, and B. dothidea, respectively. Although the efficacy of the conjugate was slightly lower, with EC90 values of 507.5, 580.2, and 497.4 µg·mL−1, respectively, it may represent a more cost-effective option to the utilization of pure ε-polylysine. The proposed treatments may offer a viable and sustainable alternative for controlling GTDs.

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Section: Discussionmentioning

confidence: 99%

Antifungal Agents Based on Chitosan Oligomers, ε-polylysine and Streptomyces spp. Secondary Metabolites against Three Botryosphaeriaceae Species

et al. 2019

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“…Thus, important efforts are needed to search for alternative control strategies to reduce costs and dependence of chemicals. Currently, Grapevine Trunk Diseases GTDs (Bertsch et al, 2013;Roblin et al, 2019) are among the most important vine destructive diseases. In economic terms, in France, losses due to these diseases are estimated at around one thousand million € per year (Lorch, 2014) in yield production terms.…”

Section: Introductionmentioning

confidence: 99%

Colonization of Vitis vinifera L. by the Endophyte Trichoderma sp. Strain T154: Biocontrol Activity Against Phaeoacremonium minimum

et al. 2020

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Trichoderma strains used in biological control products usually exhibit high efficiency in the control of plant diseases. However, their behavior under field conditions is difficult to predict. In addition, the potential of indigenous strains has been poorly assayed as well as their possible behavior as endophytes. Hence, niche colonization is a key feature for an effective protection. In this study, we aimed to: (i) explore the possibility of using a new Trichoderma strain isolated from vine to control pathogens, (ii) study the in planta interaction with the pathogen Phaeoacremonium minimum W. Gams, Crous, M.J. Wingf. & L. Mugnai (formerly Phaeoacremonium aleophilum), a pioneer fungus involved in Grapevine Trunk Diseases (GTDs) such as esca. For this purpose, fluorescently tagged Trichoderma sp. T154 and a P. minimum strain were used for scanning electron microscopy and confocal scanning laser microscopy analyses. Data showed that the Trichoderma strain is able to colonize plants up to 12 weeks post inoculation and is located in xylem, fibers, as well as in parenchymatic tissues inside the wood. The beneficial fungus reduced colonization of the esca-related pathogen colonizing the same niches. The main observed mechanism involved in biocontrol of Trichoderma against the esca pathogen was spore adhesion, niche exclusion and only few typical hypha coiling was found between Trichoderma and the pathogen. These results suggest that the Trichoderma strain has potential for reducing the colonization of Phaeoacremonium minimum and thus, an inoculation of this biological control agent can protect the plant by limiting the development of GTD, and the strain can behave as an endophyte.

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“…Diagnosis of ESCA is not easy because of the time delay between wood infection and symptom expression, the unpredictable year-to-year discontinuity in symptom expression, and the differential disease manifestations [ 29 , 41 , 44 ]. The present criteria for ESCA diagnosis consist of uprooting or cutting the vines or the invasive collection of wood samples, all of which are costly.…”

Section: Discussionmentioning

confidence: 99%

“…A typical reaction of grapevine tissues to drought is the biosynthesis of ABA, which regulates transpiration-mediated stomatal closure [ 4 , 41 ]. Consistent with the results of this study ( Figure 6 c), elevated levels of ABA have been detected in tiger-striped leaves [ 3 ].…”

Section: Discussionmentioning

confidence: 99%

“…Esca complex disease (ESCA) is another GTD that attacks the perennial organs of grapevines, leading to tiger-stripe symptoms on leaves, apoplexy of shoots [ 2 , 3 ], and extensive wood necrosis (white rot, brown streaking, and other types of dark necrotic spots) on the trunk and cordons [ 26 ] colonized by fungi, such as Phaeomoniella chlamydospora [ 5 , 27 , 28 , 29 ], Phaeoacremonium minimum [ 5 , 30 ], Fomitiporia mediterranea [ 31 , 32 ], Botryosphaeriaceae species, and other fungal taxa [ 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ]. Protection against GTDs relies mainly on prophylactic measures, remedial surgery [ 26 , 39 ], and the application of biological agents [ 8 , 31 ] or mixtures of fertilizers [ 40 , 41 ] to reduce symptom incidence and severity; these control strategies often offer only partial protection. One of the main concerns in agriculture today is the reduction of pesticide use [ 29 , 31 , 42 , 43 ].…”

Section: Introductionmentioning

confidence: 99%

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A Lipidomic Analysis of Leaves of Esca-Affected Grapevine Suggests a Role for Galactolipids in the Defense Response and Appearance of Foliar Symptoms

Goufo

Cortez

2020

Biology

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Both qualitative and quantitative changes occur in the lipid composition of Vitis vinifera L. tissues, which may compromise the defense response against Esca complex disease, a widespread and damaging trunk disease. In this study, a lipidomic analysis of grapevine leaves is conducted to assess how lipid membrane remodeling relates to the emergence and progression of Esca foliar symptoms. In total, 208 molecular species (including lipids, four hormones, and some other compounds of the metabolism of lipids) were detected. Lipid species were readily assigned to the classes fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterols, and prenol lipids. Using different clustering analyses, distinct metabolic pathways stimulated at different stages of disease development were characterized. These analyses revealed consistent changes in the abundance of 13 galactolipids and two diacylglycerolipids. Overall, the observations indicated an increment in the levels of these lipid species in leaves of asymptomatic vines and a progressive drop with increasing foliar symptom severity in symptomatic vines. Five fatty acids also appear to exert a central role in the etiopathogenesis of Esca complex disease because of their accumulation in leaves of asymptomatic vines, namely, heptadecanoic, linoleic, γ-linolenic, arachidonic, and stearic acids. Symptomatic leaves were characterized by high levels of all lipid classes, except for galactolipids, lyso-galactolipids, and compounds relevant to the biosynthesis of chlorophylls and carotenoids, that exhibited decreased levels. The data also suggested a jasmonic acid-associated signaling mechanism activation upon the invasion of woods by Esca-associated fungi, compared with abscisic and salicylic acids. Further research is required for validation of these results with additional molecular analyses using more vine cultivars.

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Towards a preventive and/or curative treatment of esca in grapevine trunk disease: General basis in the elaboration of treatments to control plant pathogen attacks

Cited by 9 publications

References 86 publications

Antifungal Agents Based on Chitosan Oligomers, ε-polylysine and Streptomyces spp. Secondary Metabolites against Three Botryosphaeriaceae Species

Antifungal Agents Based on Chitosan Oligomers, ε-polylysine and Streptomyces spp. Secondary Metabolites against Three Botryosphaeriaceae Species

Colonization of Vitis vinifera L. by the Endophyte Trichoderma sp. Strain T154: Biocontrol Activity Against Phaeoacremonium minimum

A Lipidomic Analysis of Leaves of Esca-Affected Grapevine Suggests a Role for Galactolipids in the Defense Response and Appearance of Foliar Symptoms

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