Towards a New Reference Test for Surra in Camels

Tran, Thao; Claes, Filip; Verloo, Didier; Greve, Henri De; Büscher, Philippe

doi:10.1128/cvi.00096-09

Cited by 34 publications

(19 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This rare trypanosome type was isolated for the first time from camels in Kenya and may also occur in Sudan and Ethiopia (Borst et al, 1987;Hagos et al, 2009;Salim et al, 2011). Therefore, it might be of interest (Müller et al, 1992;Nguyen et al, 2012;Rogé et al, 2013;Tran et al, 2009). Of particular interest is GM6 of which a 4 repeat fragment derived from T. evansi (TeGM6-4r) has been expressed in E. coli and incorporated in a lateral flow ICT (Nguyen et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Surra Sero K-SeT, a new immunochromatographic test for serodiagnosis of Trypanosoma evansi infection in domestic animals

Hadush

Rogé

Simon

et al. 2015

Veterinary Parasitology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Surra Sero K-SeT, a new immunochromatographic test for serodiagnosis of Trypanosoma evansi infection in domestic animals

Hadush

Rogé

Simon

et al. 2015

Veterinary Parasitology

View full text Add to dashboard Cite

“…They include the ISG family as well as various intracellular proteins such as the trypanosome-derived oligopeptidase B, trypanothion and cystein proteases. In this context, it was recently shown that ISG75 can be used as a diagnostic marker in an experimental setting, albeit the assays performed relied on ELISA rather than on a card agglutination format, and was so far only validated for the detection of animal trypanosomiasis [87].…”

Section: Review Magez and Radwanskamentioning

confidence: 99%

African Trypanosomiasis and Antibodies: Implications for Vaccination, Therapy and Diagnosis

Magez

Radwanska

2009

Future Microbiol.

View full text Add to dashboard Cite

African trypanosomiasis causes devastating effects on human populations and livestock herds in large parts of sub-Saharan Africa. Control of the disease is hampered by the lack of any efficient vaccination results in a field setting, and the severe side effects of current drug therapies. In addition, with the exception of Trypanosoma brucei gambiense infections, the diagnosis of trypanosomiasis has to rely on microscopic analysis of blood samples, as other specific tools are nonexistent. However, new developments in biotechnology, which include loop-mediated isothermal amplification as an adaptation to conventional PCR, as well as the antibody engineering that has allowed the development of Nanobody technology, offer new perspectives in both the detection and treatment of trypanosomiasis. In addition, recent data on parasite-induced B-cell memory destruction offer new insights into mechanisms of vaccine failure, and should lead us towards new strategies to overcome trypanosome defenses operating against the host immune system.

show abstract

“…Nevertheless, it failed to detect T. evansi type B which lacks or does not express RoTat1.2 (Ngaira et al 2005;Tran et al 2009). Another promising recombinant protein is the invariant surface glycoprotein 75 (ISG75) presenting in approximately 5×10 4 molecules on trypanosome cell surface (Tran et al 2009). However, limited number of available antigen candidates drives an urgent need of identification for novel antigens to improve serological diagnostics of surra.…”

Section: Introductionmentioning

confidence: 99%

“…However, preparation of the lysate antigen requires in vitro (cell culture) or in vivo (experimental animals) systems to produce sufficient number of the parasite, and procedures for antigen preparation has not been completely standardized (Reid and Copeman 2002;OIE 2008;Tran et al 2009). Therefore, development of antibody detection ELISA for surra using defined antigens, such as recombinant proteins, has long been needed.…”

Section: Introductionmentioning

confidence: 99%

Tandem repeat protein as potential diagnostic antigen for Trypanosoma evansi infection

et al. 2011

View full text Add to dashboard Cite

Trypanosoma evansi infection (surra) causes significant losses in livestock production in tropical and sub-tropical areas. The current ELISA recommended by OIE for diagnosis of the disease is based on trypanosome lysate antigen. However, antigenic variation and unstable nature of cell lysate antigen make it difficult to standardize the assay. Thus, there are needs to develop recombinant antigen-based ELISA that improve stability, sensitivity, and specificity of the test. Since tandem repeat (TR) proteins of trypanosomatid parasites generally possess high antigenicity, they have been considered to be the promising antigens for trypanosomosis and leishmaniosis. In this study, IgG responses against 14 recombinant TR proteins of trypanosomes were examined by ELISA. Serum samples were obtained from three water buffaloes experimentally infected with T. evansi. Since Trypanosoma congolense GM6 (TcoGM6) elicited highest IgG responses to all water buffaloes, we further bioinformatically and molecular biologically identified Trypanosoma brucei brucei GM6 (TbbGM6) and T. evansi GM6 (TeGM6) TR genes, respectively. As expected, predicted amino acid sequences of TbbGM6 and TeGM6 were identical while the nucleic acid sequence homology between TbbGM6 and TcoGM6 was 63.8%. All buffaloes became clearly positive in recombinant TbbGM6 (rTbbGM6)-based ELISA at 48 days post-infection, suggesting that rTbbGM6 is usable as a serodiagnostic antigen for chronic T. evansi infection.

show abstract

Towards a New Reference Test for Surra in Camels

Abstract: Current serological diagnosis of Trypanosoma evansi infection in camels is

Cited by 34 publications

References 27 publications

Surra Sero K-SeT, a new immunochromatographic test for serodiagnosis of Trypanosoma evansi infection in domestic animals

Surra Sero K-SeT, a new immunochromatographic test for serodiagnosis of Trypanosoma evansi infection in domestic animals

African Trypanosomiasis and Antibodies: Implications for Vaccination, Therapy and Diagnosis

Tandem repeat protein as potential diagnostic antigen for Trypanosoma evansi infection

Contact Info

Product

Resources

About