Towards a more rational approach to fungicide design

Schwinn, F. J.; Geissbühler, H.

doi:10.1016/0261-2194(86)90036-0

Cited by 9 publications

(1 citation statement)

References 15 publications

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“…In contrast, cutinase is one of the most important genes in plant-pathogenic fungi, and its involvement in the penetration of the barrier cuticles and in subsequent disease development has been demonstrated in several fungi (9, 10, 23, 26-28, 43, 47, 49). These findings have resulted in research targeting inactivation of the cutinase gene for plant disease control (32,45,47). The significance of cutinase in fungal pathogenicity and virulence prompted us to undertake this study.…”

Section: Discussionmentioning

confidence: 99%

Cutinase in Cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded RNAs.

Varley¹,

Podila²,

Hiremath³

1992

Mol. Cell. Biol.

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Plant-pathogenic fungi produce cutinase, an enzyme required to degrade plant cuticles and facilitate penetration into the host. The absence of cutinase or a decrease in its production has been associated with a decrease in pathogenicity of the fungus. A set of isogenic strains of Cryphonectria parasitica, the chestnut blight fungus, was tested for the presence and amounts of cutinase activity. The virulent strain of C. parasitica produced and secreted significantly higher amounts of cutinase than the hypovirulent strains. Use of both nucleic acid and polyclonal antibody probes for cutinase from Fusarium solani f. sp. pisi showed that cutinase in C. parasitica is 25 kDa in size and is coded by a 1.1-kb mRNA. Both mRNA and protein were inducible by cutin hydrolysate, while hypovirlnence agents suppressed the level of mRNA and the enzyme. Since all the strains had the cutinase gene, the suppression of expression was due to the hypovirulence agents. The data presented are the first report indicating that hypovirulence agents in C. parasitica regulate a gene associated with pathogenicity in other plant-pathogenic fungi.

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Section: Discussionmentioning

confidence: 99%

Cutinase in Cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded RNAs.

Varley¹,

Podila²,

Hiremath³

1992

Mol. Cell. Biol.

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Innovation in the Agrochemical Industry

Hond¹

2003

Pesticides

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The discovery and development of fungicides—does biochemistry have a role?

Kuhn

1989

Pestic. Sci.

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While the parts played by synthesis chemists, biologists and toxicologists in the discovery and development of fungicides are well established, the role of the biochemist is generally less well appreciated. Biochemistry does, however, have an important contribution to make, the principal activities being: mode of action studies; generation of data on the intrinsic activities of molecules; biorational design of novel inhibitors; fungicide resistance; and molecular biology and its applications. The background to each of these areas and relevance to fungicide discovery and development are described. Examples from studies on ergosterol biosynthesis inhibitors are used to illustrate the discussion.

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Towards a more rational approach to fungicide design

Cited by 9 publications

References 15 publications

Cutinase in Cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded RNAs.

Cutinase in Cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded RNAs.

Innovation in the Agrochemical Industry

The discovery and development of fungicides—does biochemistry have a role?

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