Towards a more complete glycome: Advances in ion chromatography-mass spectrometry (IC-MS) for improved separation and analysis of carbohydrates

Rumachik, Neil G.; Tian, Tian; Hou, Yuanxue; Saini, Charanjit; Cheng, Jun; Pohl, Christopher A.; Liu, Yan

doi:10.1016/j.jchromb.2021.122719

Cited by 7 publications

(3 citation statements)

References 42 publications

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“…To the best of our knowledge, information on the phosphated/sulfated lactose in milk is rather limited in literature, but important roles on mucous defense mechanism was reported by Ideo et al [42]. Hex3, Hex2HexNAc, 3'-SL and 6'-SL were determined in lactose-free milk that were separated using CarboPac™ PA300-4 μm column [37]. Likewise, we identified phosphate/sulfate ester substituted lactose as additionally.…”

Section: Structural Analysis Of Oligosaccharides By Tandem Msmentioning

confidence: 66%

“…Milk-based products are applied to several types of nutrients therefore, characterization of the milk samples is very important for the health and food industry. Bovine milk oligosaccharides shows similarity to those found in human milk and characterizing the structures of these sugars is very important for their biological profit [37,39]. In a recent study, more hexosylation (hexose-derived glycation) of amino acids on protein has been detected in lactose-free milk samples [40], with regard to these reults, characterization of glycan components of normal and lactose-free milk may contribute to the glycoproteomic studies.…”

Section: Structural Analysis Of Oligosaccharides By Tandem Msmentioning

confidence: 96%

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Glycoprofiling of Oligosaccharides of Regular and Lactose-Free Milk by Mass Spectrometry

Şahar

2022

Celal Bayar Üniversitesi Fen Bilimleri Dergisi

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Oligosaccharides from regular milk and lactose-free milk were analyzed by Electrospray Ionization interface coupled with Ion Trap Mass Spectrometry (IT-MS). The negative mode mass spectrometry of sugar compositions was obtained either by direct infusion and mass spectrometry with Liquid Chromatography (LC). Hexose (Hex) and the other oligomeric sugar components were observed in both regular and lactose-free milk. While lactose derivatives (deprotonated lactose dimer, chloride adducts, chloride dimer) in regular milk have seen to be dominated, monosaccharide derivatives (Hex-H2O, Hex, Hex-Cl) in the lactose-free milk were observed as abundant which was lytic product of lactose. Phosphate/sulfate ester substitution on lactose and sialyllactose in both regular and lactose-free milk samples were observed with similar intensity thus it has been understood that these important milk components are not digested during lactose removing process.

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Section: Structural Analysis Of Oligosaccharides By Tandem Msmentioning

confidence: 66%

Section: Structural Analysis Of Oligosaccharides By Tandem Msmentioning

confidence: 96%

Glycoprofiling of Oligosaccharides of Regular and Lactose-Free Milk by Mass Spectrometry

Şahar

2022

Celal Bayar Üniversitesi Fen Bilimleri Dergisi

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“…Ion-exchange chromatography with suppressed conductivity detection is the dominating technique for the analysis of inorganic ions and is typically referred to as IC in the analytical chemistry community [1]. However, the development of IC methods for the analysis of multifunc-tional analytes such as amino acids (AAs) [2][3][4], pesticides [5], or metabolites [6] is a focus of current research in the field. Specifically, IC-MS applications have become more and more important [7][8][9].…”

Section: Introductionmentioning

confidence: 99%

A simple ion chromatography‐mass spectrometry method for amino acid analysis in beer

Schmitt

Fillsack

Seubert

2023

J of Separation Science

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The amino acid footprint of different beer samples was analyzed using ion chromatography coupled with electrospray ionization mass spectrometry. A tailor-made polymer-based cation-exchange resin was operated with a mass spectrometry-compatible eluent under isocratic conditions on a standard highperformance liquid chromatography system coupled to a single quadrupole mass spectrometer using formic acid as a volatile eluent ion source. The partially separated peaks of the isomeric pair isoleucine/leucine were processed according to their area response ratio using vertical peak splitting or Gaussian fit. Additionally, the chromatographic resolution of the isomers was optimized with an adjusted, solely aqueous mobile phase from 0.85 to 2.92. Ion suppression in the electrospray ion source was investigated for the derivatization-free method and found to be insignificant (recovery value 100 ± 15%) for 15 out of the 20 analytes.Quantitative results for various beer and mixed-beer beverages were found to be in high agreement with existing methods. Simultaneous photometric detection demonstrated the method's ability to successfully remove most of the interfering matrix compounds.

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Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

Harvey

2024

Mass Spectrometry Reviews

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The use of matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry for the analysis of carbohydrates and glycoconjugates is a well‐established technique and this review is the 12th update of the original article published in 1999 and brings coverage of the literature to the end of 2022. As with previous review, this review also includes a few papers that describe methods appropriate to analysis by MALDI, such as sample preparation, even though the ionization method is not MALDI. The review follows the same format as previous reviews. It is divided into three sections: (1) general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, quantification and the use of computer software for structural identification. (2) Applications to various structural types such as oligo‐ and polysaccharides, glycoproteins, glycolipids, glycosides and biopharmaceuticals, and (3) other general areas such as medicine, industrial processes, natural products and glycan synthesis where MALDI is extensively used. Much of the material relating to applications is presented in tabular form. MALDI is still an ideal technique for carbohydrate analysis, particularly in its ability to produce single ions from each analyte and advancements in the technique and range of applications show little sign of diminishing.

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Towards a more complete glycome: Advances in ion chromatography-mass spectrometry (IC-MS) for improved separation and analysis of carbohydrates

Cited by 7 publications

References 42 publications

Glycoprofiling of Oligosaccharides of Regular and Lactose-Free Milk by Mass Spectrometry

Glycoprofiling of Oligosaccharides of Regular and Lactose-Free Milk by Mass Spectrometry

A simple ion chromatography‐mass spectrometry method for amino acid analysis in beer

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2021–2022

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