Towards a molecular understanding of endosomal trafficking by Retromer and Retriever

Abstract

www.traf c.dkCover legend: Retromer is essen al in all eukaryotes for the traffi cking of transmembrane proteins within membrane tubules. Cryoelectron microscopy was used to show how retromer assembles with membrane-bound sor ng nexin proteins to form these membrane tubules. The picture shows a modelled segment of the retromersor ng nexin coat. See Chen et al. (Traffi c 2019; 20(7):465-478). Read the full ar cle on Aim and ScopeTraffic encourages and facilitates the publication of papers covering the cell biology of intracellular transport in health and disease. Areas of interest include protein, nucleic acid and lipid traffic, molecular motors, intracellular pathogens, intracellular proteolysis, nuclear import and export, cytokinesis and the cell cycle, protein translocation, antigen processing and presentation, organelle biogenesis, metabolism, cell polarity and organization, and organelle movement.All aspects of the structural, molecular biology, biochemistry, genetics, morphology, intracellular signaling and relationship to hereditary or infectious diseases will be covered. Manuscripts must provide a clear conceptual or mechanistic advance. The editors will reject papers that require major changes, including addition of significant experimental data or other significant revision.

Retrograde transport of mannose-6-phosphate receptor depends on several sorting machineries as analyzed by sulfatable nanobodies

“…Accordingly, we found a clear reduction of nanobody sulfation upon knockdown of Vps26, confirming the role of retromer in retrograde transport also for CDMPR. There are many reports for a requirement of retromer for CIMPR (Arighi et al, 2004;Bugarcic et al, 2011;Bulankina et al, 2009;Chen et al, 2019;Cui et al, 2019;Fjorback et al, 2012;Hao et al, 2013;Harbour et al, 2010;Hirst et al, 2018;McGough et al, 2014;Seaman, 2004;Seaman, 2007;Seaman et al, 2009;Seaman, 2018;Wang et al, 2018;Wassmer et al, 2007). However, the requirement of the trimeric Vps retromer complex for CIMPR retrieval has recently been challenged by reports of the Cullen and Steinberg labs (Kvainickas et al, 2017;Simonetti et al, 2017).…”

Retromer forms low order oligomers on supported lipid bilayers

“…Retromer is an evolutionarily conserved protein complex that orchestrates sorting and export of integral membrane proteins from the endosome. Loss of Retromer function, which is implicated in a variety of disease conditions, results in increased rates of turnover of plasma membrane proteins and retrograde cargo proteins in the lysosome, with broad consequences to cell and organism physiology (1)(2)(3).…”

Opposing functions for retromer and Rab11 in extracellular vesicle cargo traffic at presynaptic terminals

“…Retromer, a heterotrimeric complex composed of Vps35, Vps26, and Vps29, is a central component of the endosomal trafficking machinery, and mediates cargo sorting into membrane tubules and buds (Chen et al, 2019a;McNally and Cullen, 2018;Wang et al, 2018). Retromer serves a wide variety of cellular functions, including recycling numerous cargoes from endosomes to the plasma membrane (Wang et al, 2014), retrieving lysosomal/vacuolar hydrolase receptors to protect them from degradation (Arighi et al, 2004;Cui et al, 2019;Seaman et al, 1998), facilitating autophagy (Jimenez-Orgaz et al, 2017), and influencing mitochondria fusion and function (Tang et al, 2015).…”