Towards a model for Flavobacterium gliding

Shrivastava, Abhishek; Berg, Howard C.

doi:10.1016/j.mib.2015.07.018

Cited by 29 publications

(19 citation statements)

References 30 publications

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“…SprB provides the adhesive, and rotary motors power the motion (8). As is evident from experiments or models presented by us and others (4,5,9,10,(19)(20)(21)(22), mobile cell-surface components are essential for gliding. The biochemical features of spiral tracks on which such components are loaded and their mode of integration with the rotary motor are not known.…”

Section: Discussionmentioning

confidence: 96%

“…Such movement is divided into two categories: 1) twitching and 2) gliding. Twitching involves the extension and retraction of type IV pili (1-3), but gliding bacteria do not use type IV pili (4)(5)(6)(7). Although the mechanism for gliding is barely understood, we know that Flavobacterium johnsoniae, which exhibits some of the fastest gliding of all known bacteria, has a powerful rotary motor (8) and a mobile cell-surface adhesin, SprB (9)(10)(11).…”

Section: Introductionmentioning

confidence: 99%

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The Screw-Like Movement of a Gliding Bacterium Is Powered by Spiral Motion of Cell-Surface Adhesins

Shrivastava

Roland

Berg

2016

Biophysical Journal

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Flavobacterium johnsoniae, a rod-shaped bacterium, glides over surfaces at speeds of~2 mm/s. The propulsion of a cell-surface adhesin, SprB, is known to enable gliding. We used cephalexin to generate elongated cells with irregular shapes and followed their displacement in three dimensions. These cells rolled about their long axes as they moved forward, following a right-handed trajectory. We coated gold nanoparticles with an SprB antibody and tracked them in three dimensions in an evanescent field where the nanoparticles appeared brighter when they were closer to the glass. The nanoparticles followed a righthanded spiral trajectory on the surface of the cell. Thus, if SprB were to adhere to the glass rather than to a nanoparticle, the cell would move forward along a right-handed trajectory, as observed, but in a direction opposite to that of the nanoparticle.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

The Screw-Like Movement of a Gliding Bacterium Is Powered by Spiral Motion of Cell-Surface Adhesins

Shrivastava

Roland

Berg

2016

Biophysical Journal

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“…Several conveyor belts may be arranged in patterns that connect to each other like treads that link sprockets in a snowmobile; this might give the movement of adhesins a helical appearance. Unlike the baseplate relay model, this ‘ snowmobile ’ model would only require a few motor units (Shrivastava and Berg, ) (Fig. B).…”

Section: Flavobacterial Gliding Couples a Rotary Motor To A Unique Sementioning

confidence: 99%

Novel mechanisms power bacterial gliding motility

Nan

Zusman

2016

Molecular Microbiology

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Summary For many bacteria, motility is essential for survival, growth, virulence, biofilm formation and intra/interspecies interactions. Since natural environments differ, bacteria have evolved remarkable motility systems to adapt, including swimming in aqueous media, and swarming, twitching and gliding on solid and semi-solid surfaces. Although tremendous advances have been achieved in understanding swimming and swarming motilities powered by flagella, and twitching motility powered by Type IV pili, little is known about gliding motility. Bacterial gliders are a heterogeneous group containing diverse bacteria that utilize surface motilities that do not depend on traditional flagella or pili, but are powered by mechanisms that are less well understood. Recently, advances in our understanding of the molecular machineries for several gliding bacteria revealed the roles of modified ion channels, secretion systems and unique machinery for surface movements. These novel mechanisms provide rich source materials for studying the function and evolution of complex microbial nano-machines. In this review, we summarize recent findings made on the gliding mechanisms of the myxobacteria, flavobacteria and mycoplasmas.

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“…How the molecular players form the machinery that actuates this motion is not clearly understood. F. johnsoniae is now the organism of choice for studies of this process because rates of movement are high, and much of the genetics is known (7,8). A mobile cell-surface adhesin, SprB, has been identified, which plays a central role in gliding (9,10).…”

Section: Introductionmentioning

confidence: 99%

A molecular rack and pinion actuates a cell-surface adhesin and enables bacterial gliding motility

Shrivastava

Berg

2020

Sci. Adv.

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The gliding bacterium Flavobacterium johnsoniae is known to have an adhesin, SprB, that moves along the cell surface on a spiral track. Following viscous shear, cells can be tethered by the addition of an anti-SprB antibody, causing spinning at 3 Hz. Labeling the type 9 secretion system (T9SS) with a YFP fusion of GldL showed a yellow fluorescent spot near the rotation axis, indicating that the motor driving the motion is associated with the T9SS. The distance between the rotation axis and the track (90 nm) was determined after adding a Cy3 label for SprB. A rotary motor spinning a pinion of radius 90 nm at 3 Hz would cause a spot on its periphery to move at 1.5 μm/s, the gliding speed. We suggest the pinion drives a flexible tread that carries SprB along a track fixed to the cell surface. Cells glide when this adhesin adheres to the solid substratum.

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Towards a model for Flavobacterium gliding

Cited by 29 publications

References 30 publications

The Screw-Like Movement of a Gliding Bacterium Is Powered by Spiral Motion of Cell-Surface Adhesins

The Screw-Like Movement of a Gliding Bacterium Is Powered by Spiral Motion of Cell-Surface Adhesins

Novel mechanisms power bacterial gliding motility

A molecular rack and pinion actuates a cell-surface adhesin and enables bacterial gliding motility

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