Towards a light-addressable transducer bacteriorhodopsin based

Nicolini, Claudio; Erokhin, Victor; Paddeu, Sergio; Sartore, Marco

doi:10.1088/0957-4484/9/3/013

Cited by 18 publications

(12 citation statements)

References 30 publications

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“…Bacteriorhodopsin (bR) and other light‐sensitive proteins, including visual pigments (rhodopsin) from bovine,1–4 photosynthetic reaction centers5 are of interest for use in biotechnology and molecular electronic applications 6–10. The unique combination of photochromic, photoelectric and electrooptical properties of these proteins is of great fundamental and applied interest.…”

Section: Introductionmentioning

confidence: 99%

“…Bacteriorhodopsin (bR) and other light-sensitive proteins, including visual pigments (rhodopsin) from bovine, [1][2][3][4] photosynthetic reaction centers 5 are of interest for use in biotechnology and molecular electronic applications. [6][7][8][9][10] The unique combination of photochromic, photoelectric and electrooptical properties of these proteins is of great fundamental and applied interest. bR is a promising candidate for information processing because of its astonishing stability against chemical, thermal and photochemical degradation as well as its efficient photochemistry and excellent reversibility.…”

Section: Introductionmentioning

confidence: 99%

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Photoreversibility and photostability in films of octopus rhodopsin isolated from octopus photoreceptor membranes

Paternolli¹,

Neebe²,

Stura³

et al. 2008

J Biomedical Materials Res

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In this work, a new biomaterial resulting from the isolation of octopus rhodopsin (OR) starting from octopus photoreceptor membranes is presented. Mass spectroscopic characterization was employed in order to verify the presence of rhodopsin in the extract. Photoreversibility and photochromic properties were investigated using spectrophotometric measurements and pulsed light. Thin films of OR were realized using the gel-matrix entrapment method in polyvinyl alcohol solution. The results indicate that the photoreversibility and the photostability of the OR in gel-matrices are maintained. Several measurements were performed to test the stability of the resulting biomaterial in time and at room temperature. Preliminary tests demonstrate that the photoreversibility and the photostability are still found after few days from the biomaterial preparation and after the exposure for several hours at room temperature.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Photoreversibility and photostability in films of octopus rhodopsin isolated from octopus photoreceptor membranes

Paternolli¹,

Neebe²,

Stura³

et al. 2008

J Biomedical Materials Res

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“…As theˇrst step, we build a 3D model of octR using bovR as a template, and compare it with the structure of bR. We also discuss this comparison in terms of nanotechnology applications that have been reported for bR [31] but can also be implemented in octR. We also built a model for mutant protein aimed at ocrR/bR interconversion.…”

Section: Introductionmentioning

confidence: 99%

Prospects for octopus rhodopsin utilization in optical and quantum computation

Sivozhelezov

Nicolini

2007

Phys. Part. Nuclei Lett.

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Visual membranes of octopus, whose main component is the light-sensitive signal transducer octopus rhodopsin (octR), are extremely highly ordered, easily capture single photons, and are sensitive to light polarization, which shows their high potential for use as a QC detector. However, artiˇcial membranes made of octR are neither highly enough ordered nor stable, while the bacterial homolog of octR, bacteriorhodopsin (bR), having the same topology as octR, forms both stable and ordered artiˇcial membranes but lacks the optical properties important for optical QC. In this study, we investigate the structural basis for ordering of the two proteins in membranes in terms of crystallization behavior. We compare atomic resolution 3D structures of octR and bR and show the possibility for structural bR/octR interconversion by mutagenesis. We also show that the use of (nano)biotechnology can allow (1) high-precision manipulation of the light acceptor, retinal, including converting its surrounding into that of bacterial rhodopsin, the protein already used in optical-computation devices and (2) development of multicomponent and highly regular 2D structures with a high potential for being efˇcient optical QC detectors.

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“…21 The importance of active transport systems has led to the isolation and detailed study of several transporter proteins such as bR and their reconstitution in liposomes, vesicles, and supported lipid bilayers. 22,23 Recently, Luo et al demonstrated the immobilization and functionality of proteoliposomes ͑bR containing liposomes͒ in silica matrices. 15 Gramicidin, a model ion channel, is an unusual transmembrane peptide, with alternating D and L amino acids.…”

Section: Introductionmentioning

confidence: 99%

Robust hybrid thin films that incorporate lamellar phospholipid bilayer assemblies and transmembrane proteins

2006

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This study describes facile methods based on sol-gel processing for the formation of robust thin films that incorporate phospholipid bilayer membranes and transmembrane proteins as multilamellar assemblies in cross-linked silica matrices. Transmission electron microscopy and x-ray diffraction were used to examine the lamellar structure of the hybrid thin films containing 1, 2-dioleyl-sn-glycero-3-phospoethanolamine (DOPE), an unsaturated lipid, and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), a saturated lipid. While the d spacing measured for DOPE containing films varied (from 35 to 48 Å) depending on the amount of DOPE added to the coating solution (10-1 wt %), similar changes were not observed for the films containing saturated lipid, DMPC (d spacing ∼43 Å). Addition of purple membrane containing bacteriorhodopsin to the DOPE/silica coating solution led to the formation of multilamellar vesicle-like structures within the thin films. Mild sonication of these solutions containing the purple membrane prior to coating led to the formation thin films with planar multilamellar structures that exhibit uniform d spacing. The study further investigates the effects of incorporation of gramicidin and sonication on the structure of hybrid films and speculates on the eventual application of thin films prepared in this manner.

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Towards a light-addressable transducer bacteriorhodopsin based

Cited by 18 publications

References 30 publications

Photoreversibility and photostability in films of octopus rhodopsin isolated from octopus photoreceptor membranes

Photoreversibility and photostability in films of octopus rhodopsin isolated from octopus photoreceptor membranes

Prospects for octopus rhodopsin utilization in optical and quantum computation

Robust hybrid thin films that incorporate lamellar phospholipid bilayer assemblies and transmembrane proteins

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