Towards a green enantiomeric esterification of R/S-ketoprofen: A theoretical and experimental investigation

Lipase B of Candida antarctica co‐adsorbed with polyols onto TiO2 nanoparticles for improved biocatalytic performance

“…The catalytic activity of the immobilized lipase over the TiO 2 NPs is negligible when just ethanol is used as reactant and solvent in the reaction. This observation is attributed to the inhibitory effect of the alcohol reported previously in this esterification reaction in particular, and extensively studied by different kinetic measurements, suggesting that the alcohol has the effect of a competitive inhibitor according to its concentration …”

“…Similarly, the contribution of the β‐turn structure corresponds to the addition of the areas of the signals located at 1664 cm −1 , 1666 cm −1 and 1676 cm −1 . Finally, a signal at 1619 cm −1 is ascribed to β aggregates …”

“…The percentage contribution of each structure was obtained through the deconvolution, integration and further normalization of the corresponding signals involved in the Amide I. The details of the methodology were published before …”

“…The conversion of ibuprofen and the enantiomeric excess towards the S(+)‐enantiomer were determined through titration with potassium hydroxide and chiral HPLC analysis, respectively. Details of the analysis are published elsewhere . The specific activity was calculated as the micromols of ibuprofen converted to esters per milligram of protein per time of reaction in minutes.…”

“…However, it has been demonstrated that short chain alcohols (methanol, ethanol, 1‐ and 2‐propanol) dissolve the macroporous resin . With this in mind, the use of alternative supports, such as metal oxides, represents an interesting alternative.…”