Towards a Global Barcode Library for Lymantria (Lepidoptera: Lymantriinae) Tussock Moths of Biosecurity Concern

deWaard, Jeremy R; Mitchell, Andrew; Keena, Melody A.; Gopurenko, David; Boykin, Laura M.; Armstrong, Karen; Pogue, Michael G.; Lima, J. A. S.; Floyd, Robin; Hanner, Robert; Humble, Leland M.

doi:10.1371/journal.pone.0014280

Cited by 72 publications

(66 citation statements)

References 45 publications

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“…Our finding that males of L. schaeferi were attracted to (+)-xylinalure, but not to (+)-disparlure, supports molecular evidence (deWaard et al 2010) that L. schaeferi is more closely related to L. xylina, which uses (+)-xylinalure for sexual communication (Gries et al 1999) than it is to L. dispar, which uses (+)-disparlure for sexual communication (Bierl et al 1970). However, we have yet to analyze the sex pheromone of female L. schaeferi and to confirm that they produce (+)-xylinalure.…”

Section: Resultssupporting

confidence: 81%

“…and L. schaeferi (deWaard et al, 2010). Here we present further evidence that these two species are closely related.…”

supporting

confidence: 74%

“…Following export of trap-captured specimens, additional genetic analyses (deWaard et al 2010) and placement of voucher specimens will be completed. (+)-xylinalure as a trap lure may now prove useful for further studies into the behavior of L. schaeferi.…”

Section: Resultsmentioning

confidence: 99%

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Attraction of male <i>Lymantria schaeferi</i> Schintlmeister (Lepidoptera: Erebidae: Lymantriinae) to traps baited with (+)-xylinalure in Jiangxi Province, China

Schaefer¹,

Jiang²,

Gries

et al. 2013

Life: The Excitement of Biology

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Abstract:Our objective was to investigate potential sex attractants for Lymantria schaeferi Schintlmeister (Lexpidoptera: Erebidae: Lymantriinae). In a field trapping experiment deployed in the Wuyi Mountains near Xipaihe, Jiangxi Province, China, traps were baited with synthetic sex pheromone of congeners Lymantria schaeferi (Figure 1) and L. xylina (Figure 2) are not sympatric in China and males of these two species are morphologically distinct from one another. Males of L. xylina are predominantly white, whereas males of L. schaeferi are dark brown and resemble males of L. dispar except that they bear pink setae on their legs, venter of the thorax, and on the pronotum behind the head. The pink coloration mentioned is common to both L. schaeferi and L. xylina. Furthermore, genetic analysis of a single specimen of L. schaeferi (provided for analysis by A. Schintlmeister from the paratype series) led to the conclusion there is only 0.2% molecular sequence divergence between L. xylina

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Section: Resultssupporting

confidence: 81%

“…and L. schaeferi (deWaard et al, 2010). Here we present further evidence that these two species are closely related.…”

supporting

confidence: 74%

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Attraction of male <i>Lymantria schaeferi</i> Schintlmeister (Lepidoptera: Erebidae: Lymantriinae) to traps baited with (+)-xylinalure in Jiangxi Province, China

Schaefer¹,

Jiang²,

Gries

et al. 2013

Life: The Excitement of Biology

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“…This will become increasingly important in meeting standards of national and international regulation in fields such as agriculture, forestry and fisheries (Jones, F. C., 2008;de Waard et al, 2010;Floyd et al, 2010;Boykin et al, 2012), as well as emerging rules for access and benefit sharing under the Convention on Biological Diversity (Schindel, 2010). Newmaster and Ragupathy (2010) have applied DNA barcoding to quality control on traditional knowledge in ethnobotany, to test identifications of plants and to explore the presence of cryptic species.…”

Section: Contribution Of Barcoding To Quality Controlmentioning

confidence: 99%

DNA barcoding in floral and faunal research

Miller¹

2015

Descriptive Taxonomy

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As I write this chapter in mid 2012, the context in which floral and faunal research is done is changing rapidly. Demand for biodiversity information, especially to understand global change, is increasing. The technologies that are available for carrying out biodiversity research and for disseminating the results are changing dramatically. The funding processes and organisational cultures of the institutions that have been the traditional homes of such research are evolving. This creates new challenges and opportunities for floral and faunal research. This chapter will focus on the intersection of DNA barcoding with floral and faunal research, the opportunities that DNA barcoding offers for increasing the quality and quantity of such work, and its connectivity with related activities. I will discuss the opportunities for DNA barcoding and then provide an example from my own research. The essay focuses on plants and animals, but will include some reference to other organisms. Because of the rapid evolution of the underlying technologies, and the related social changes, this essay represents a 'slice of time', and I expect some of the conclusions will be out of date before it is published. While challenges remain, I believe this is an exciting time of renaissance for taxonomy (Miller, 2007).A DNA barcode is a short gene sequence taken from standardised portions of the genome, used to identify species. Being DNA based, it can be used for specimens without morphological characters necessary for traditional identifications, such as roots or immature insects. Being a short sequence, it can be extracted from material that has not been specially preserved, and can often be extracted from standard museum or herbarium specimens. Being from a standardised region allows the use of universal primers for unknown taxa and allows rapid compilation of a global reference library. The choice of gene regions has focused on species level identification. While barcoding is not intended as a tool for higher classification research or for population genetics research, sometimes the barcoding gene regions have useful information at those levels (Craft et al., 2010). The present status of DNA barcodingWhile molecular diagnostics have been used for many years, DNA barcoding as a global initiative started by Hebert et al. (2003), who, among other things, pointed out that a standardised choice of gene region for species-level diagnosis allows individual projects of whatever scale to contribute to a global reference library that becomes increasingly more powerful over time. Mitochondrial Cytochrome c oxidase I (COI) had been widely used in animals since Folmer et al. (1994) and was quickly adopted as a community standard following Hebert et al. (2003). Standardised gene regions for plants proved more challenging for technical reasons, but are now designated as rbcL and matK, with additional research on trnH-psbA (Hollingsworth et al., 2009). The nuclear ribosomal internal transcribed spacer (ITS) region has been selected as a universal DNA b...

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“…Recent DNA work on lymantriid moths [39] [40]. The other, less widely distributed gypsy moths of the subgenus Porthetria in Asia, Lymantria albescens, L. postalba, L. umbrosa, and L. xylina, could be studied at a later date.…”

Section: Possible Sources Of Variability In Virus Activitymentioning

confidence: 99%

Comparison of the Activities of Three LdMNPV Isolates in the Laboratory Against the Chinese Strain of Asian Gypsy Moth

L.Q.¹,

I.S.²,

L.B.³

et al. 2011

TOENTOJ

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Three isolates of gypsy moth [Lymantria dispar (L.)] nucleopolyhedrovirus (LdMNPV), from China (LdMNPV-H), Japan (LdMNPV-J) and the registered strain from North America (LdMNPV-D), were bioassayed in the Inner Mongolia Autonomous Region, People's Republic of China, in 2004, using a modified version of the diet plug method, to determine their pathogenicity and virulence based on the dose-and time-response of second-instar larvae of the Chinese strain of the Asian gypsy moth. Results showed that LdMNPV-H and LdMNPV-D did not differ significantly in dose-response. The LD 50 and LD 95 for LdMNPV-H were 211 and 1414 OBs larva -1 , respectively, while LdMNPV-D had a somewhat lower LD 50 (194 OBs larva -1 ) and a higher LD 95 (1705 OBs larva -1 ). LdMNPV-J was the least pathogenic, with a LD 50 of 940 OBs larva -1 and LD 95 of 11 457 OBs larva -1 . Overall, LdMNPV-H and LdMNPV-D had similar pathogenicity against second-instar larvae of the Chinese strain of the Asian gypsy moth, and were considerably more pathogenic than LdMNPV-J. Time-responses were not significantly different among the three virus strains.

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Towards a Global Barcode Library for Lymantria (Lepidoptera: Lymantriinae) Tussock Moths of Biosecurity Concern

Cited by 72 publications

References 45 publications

Attraction of male <i>Lymantria schaeferi</i> Schintlmeister (Lepidoptera: Erebidae: Lymantriinae) to traps baited with (+)-xylinalure in Jiangxi Province, China

Attraction of male <i>Lymantria schaeferi</i> Schintlmeister (Lepidoptera: Erebidae: Lymantriinae) to traps baited with (+)-xylinalure in Jiangxi Province, China

DNA barcoding in floral and faunal research

Comparison of the Activities of Three LdMNPV Isolates in the Laboratory Against the Chinese Strain of Asian Gypsy Moth

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