Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos

Reyes, Álvaro Plaza; Lanner, Fredrik

doi:10.1242/dev.139683

Cited by 49 publications

(15 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, it has been employed in several attempts to edit the human embryos (Liang et al, 2015 ; Kang et al, 2016 ; Tang et al, 2017 ). A major technical concern particularly relevant in studies involving human embryos is the potential off-target effects (Callaway, 2016 ; Plaza Reyes and Lanner, 2017 ). Consequently, development of safer genome editing strategy in human embryos is highly anticipated (Cyranoski and Reardon, 2015 ).…”

mentioning

confidence: 99%

Highly efficient and precise base editing in discarded human tripronuclear embryos

Liu

Zeng

et al. 2017

Protein Cell

View full text Add to dashboard Cite

mentioning

confidence: 99%

Highly efficient and precise base editing in discarded human tripronuclear embryos

Liu

Zeng

et al. 2017

Protein Cell

View full text Add to dashboard Cite

“…One major question is whether or not the body’s immune response will accept or reject the foreign genetic elements within the cells. Another important concern is that along with the revolutionary advances of this biotechnology and related sciences, bioethical concerns and legal problems related to this issue are still increasing in view of the possibility of human genetic manipulation and the unsafety of procedures involved [ 49 , 50 , 66 ]. The enforcement of technical and ethical guidelines, and legislations should be considered and need serious attention as soon as possible.…”

Section: Discussionmentioning

confidence: 99%

“…Engineered endonucleases or RNA-guided endonucleases (RGENs) mediated gene targeting has been applied directly in a great number of animal organisms including nematodes and zebrafish [ 20 , 57 ], as well as pigs [ 71 , 85 ]. Since the first permission to use CRISPR/Cas9 in human embryos and in vivo genome editing via homology-independent targeted integration (HITI), an increasing number of studies have identified striking differences between mouse and human pre-implantation development and pluripotency [ 66 ], highlighting the need for focused studies in human embryos. Therefore, more specific criteria and widely accepted standards for clinical research have to be met before human germline editing would be deemed permissible [ 31 ].…”

Section: Applications Of Gene Technologymentioning

confidence: 99%

The genome editing revolution: review

Khalil

2020

Journal of Genetic Engineering and Biotechnology

158

View full text Add to dashboard Cite

Background Development of efficient strategies has always been one of the great perspectives for biotechnologists. During the last decade, genome editing of different organisms has been a fast advancing field and therefore has received a lot of attention from various researchers comprehensively reviewing latest achievements and offering opinions on future directions. This review presents a brief history, basic principles, advantages and disadvantages, as well as various aspects of each genome editing technology including the modes, applications, and challenges that face delivery of gene editing components. Main body Genetic modification techniques cover a wide range of studies, including the generation of transgenic animals, functional analysis of genes, model development for diseases, or drug development. The delivery of certain proteins such as monoclonal antibodies, enzymes, and growth hormones has been suffering from several obstacles because of their large size. These difficulties encouraged scientists to explore alternative approaches, leading to the progress in gene editing. The distinguished efforts and enormous experimentation have now been able to introduce methodologies that can change the genetic constitution of the living cell. The genome editing strategies have evolved during the last three decades, and nowadays, four types of “programmable” nucleases are available in this field: meganucleases, zinc finger nucleases, transcription activator-like effector nucleases, and the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) (CRISPR/Cas-9) system. Each group has its own characteristics necessary for researchers to select the most suitable method for gene editing tool for a range of applications. Genome engineering/editing technology will revolutionize the creation of precisely manipulated genomes of cells or organisms in order to modify a specific characteristic. Of the potential applications are those in human health and agriculture. Introducing constructs into target cells or organisms is the key step in genome engineering. Conclusions Despite the success already achieved, the genome editing techniques are still suffering certain difficulties. Challenges must be overcome before the full potential of genome editing can be realized.

show abstract

“…However, laboratories across the globe are now using this technology for innovative approaches in the field of biology. [22] FIG 3 :-Synthego output example with PAM sequence, Guide sequence, and the target region…”

Section: Crispr/cas9 Technologymentioning

confidence: 99%

Role of CRISPR/Cas9 in Genetic Manipulation of ROS1 and EGFR Genes using Synthego Platform

Goenka¹,

De²,

Biswas³

2020

IJISRT

View full text Add to dashboard Cite

Mutations and fusions in kinase enzymes are often observed in cancer prognosis. The growth and survival of tumor cells depend on the activation of kinase enzymes which when activated unrestrained can lead to the uncontrolled division of malignant lung cells. Thus, their inhibition is viewed as a promising and effective anti-cancer therapy. ROS1 and EGFR are two tyrosine kinases that have been explored as the genes responsible for Non-Small Cell Lung Cancer (NSCLC). By interrupting the unchecked division of these genes, the development of malignant lung cancer cells can be blocked. The results show 4 of the top-line RNAs for altering the gene quality as well as the target sequences relevant to cleavage by that gRNA, 4 for each gene. We propose a genetic approach of controlling the ROS1 and EGFR genes guided by CRISPR/Cas-9 to guarantee fewer symptoms and an increasingly powerful treatment, by the use of computational tools.

show abstract

Towards a CRISPR view of early human development: applications, limitations and ethical concerns of genome editing in human embryos

Cited by 49 publications

References 53 publications

Highly efficient and precise base editing in discarded human tripronuclear embryos

Highly efficient and precise base editing in discarded human tripronuclear embryos

The genome editing revolution: review

Role of CRISPR/Cas9 in Genetic Manipulation of ROS1 and EGFR Genes using Synthego Platform

Contact Info

Product

Resources

About