Towards a blocking-free electrochemical immunosensing strategy for anti-transglutaminase antibodies using screen-printed electrodes

Martín‐Yerga, Daniel; Costa-Garcı́a, Agustı́n

doi:10.1016/j.bioelechem.2015.05.014

Cited by 27 publications

(13 citation statements)

References 45 publications

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“…We aimed to apply our previous assay [ 28 ] to the 8-channel array of electrodes in order to perform multiple simultaneous analysis. This array of electrodes has been successfully employed in numerous applications based on electrochemical biosensors [ 10 , 11 , 12 , 13 , 14 , 15 , 29 , 30 ] while presenting their great advantages such as low sample requirement and easy implementation to point of care (PoC) system. The characterization of the carbon nanofiber enriched electrodes has been given in earlier reports [ 28 , 31 , 32 , 33 ].…”

Section: Resultsmentioning

confidence: 99%

Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array

Erdem

Ekşin

2020

Biosensors

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A carbon nanofiber enriched 8-channel screen-printed electrochemical array was used for the impedimetric detection of SNP related to Factor V Leiden (FV Leiden) mutation, which is the most common inherited form of thrombophilia. FV Leiden mutation sensing was carried out in three steps: solution-phase nucleic acid hybridization between zip nucleic acid probe (Z-probe) and mutant type DNA target, followed by the immobilization of the hybrid on the working electrode area of array, and measurement by electrochemical impedance spectroscopy (EIS). TArzhe selectivity of the assay was tested against mutation-free DNA sequences and synthetic polymerase chain reaction (PCR) samples. The developed biosensor was a trustful assay for FV Leiden mutation diagnosis, which can effectively discriminate wild type and mutant type even in PCR samples.

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Section: Resultsmentioning

confidence: 99%

Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array

Erdem

Ekşin

2020

Biosensors

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“…In principle, such barcodes could be used for the simultaneous detection of thousands of biomarkers using computational deconvolution. The most widespread practice in detecting QDs is through ASV proceeded by acid digestion [ 82 , 83 ]. Merkoci et al were among the first to demonstrate a less aggressive strategy by direct detection of CdS through the redox cycling of Cd (II).…”

Section: Immunoassay Techniques For Printed Electrodesmentioning

confidence: 99%

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

et al. 2020

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Medical diagnostics is trending towards a more personalized future approach in which multiple tests can be digitized into patient records. In cancer diagnostics, patients can be tested for individual protein and genomic biomarkers that detect cancers at very early stages and also be used to monitor cancer progression or remission during therapy. These data can then be incorporated into patient records that could be easily accessed on a cell phone by a health care professional or the patients themselves on demand. Data on protein biomarkers have a large potential to be measured in point-of-care devices, particularly diagnostic panels that could provide a continually updated, personalized record of a disease like cancer. Electrochemical immunoassays have been popular among protein detection methods due to their inherent high sensitivity and ease of coupling with screen-printed and inkjet-printed electrodes. Integrated chips featuring these kinds of electrodes can be built at low cost and designed for ease of automation. Enzyme-linked immunosorbent assay (ELISA) features are adopted in most of these ultrasensitive detection systems, with microfluidics allowing easy manipulation and good fluid dynamics to deliver reagents and detect the desired proteins. Several of these ultrasensitive systems have detected biomarker panels ranging from four to eight proteins, which in many cases when a specific cancer is suspected may be sufficient. However, a grand challenge lies in engineering microfluidic-printed electrode devices for the simultaneous detection of larger protein panels (e.g., 50–100) that could be used to test for many types of cancers, as well as other diseases for truly personalized care.

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“…Other interesting electrochemical immunosensors for the detection of anti-tTG IgA and anti-tTG IgG in human serum samples were developed by Martin-Yerga et al [49,50]. An array of 8-channel SPEs was used as transducer and modified with tTG by adsorption.…”

Section: No Label-free Innovative Electrochemical Immunosensorsmentioning

confidence: 99%

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

Arduini

Micheli

Moscone

et al. 2016

TrAC Trends in Analytical Chemistry

329

166

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A B S T R A C TThis review addresses recent advances in the development of screen-printed electrode based biosensors modified with different nanomaterials such as carbon nanotubes, graphene, metallic nanoparticles as gold, silver and magnetic nanoparticles, and mediator nanoparticles (Prussian Blue, Cobalt Phthalocyanine, etc.), coupled with biological recognition elements such as enzymes, antibodies, DNA and aptamers to obtain probes with improved analytical features. Examples of clinical applications are illustrated, together with examples of paper-based electrochemical devices, of multiple detections using arrays of screen printed electrodes, and of the most recent developments in the field of wearable biosensors. Also the use of smartphones as final detectors is briefly depicted.

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Towards a blocking-free electrochemical immunosensing strategy for anti-transglutaminase antibodies using screen-printed electrodes

Cited by 27 publications

References 45 publications

Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array

Impedimetric Sensing of Factor V Leiden Mutation by Zip Nucleic Acid Probe and Electrochemical Array

Printed Electrodes in Microfluidic Arrays for Cancer Biomarker Protein Detection

Electrochemical biosensors based on nanomodified screen-printed electrodes: Recent applications in clinical analysis

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