Towards a better understanding of salivary and meat juice acute phase proteins determination in pigs: An expression study

Soler, Laura; Gutiérrez, Ana María; Müllebner, Andrea; Cerón, José J.; Duvigneau, J.C.

doi:10.1016/j.vetimm.2013.09.018

Cited by 14 publications

(7 citation statements)

References 32 publications

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“…Acute phase proteins such as haptoglobin, C‐reactive protein, major acute phase protein and serum amyloid A have been designated as putative biomarkers to evaluate pig health status (Heegaard et al., , ; Hulten, Johansson, Fossum, & Wallgren, ), and their increase upon infection was observed in serum (Gómez‐Laguna et al., ; Skovgaard et al., ), saliva and meat juice (Gómez‐Laguna et al., ; Soler, Gutierrez, Mullebner, Ceron, & Duvigneau, ). Higher levels of fetuin A, as well as lower levels of haptoglobin and surfactant protein D, were suggested as biomarkers of the innate immune system for a higher resistance of pigs against App infection, mainly due to preventing excessive immune reactions detrimental to the host (Kahlisch et al., ).…”

Section: Immune Response To Infectionmentioning

confidence: 99%

Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

Sassu

Bossé

Tobias

et al. 2017

Transbound Emerg Dis

144

178

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Summary Porcine pleuropneumonia, caused by the bacterial porcine respiratory tract pathogen Actinobacillus pleuropneumoniae, leads to high economic losses in affected swine herds in most countries of the world. Pigs affected by peracute and acute disease suffer from severe respiratory distress with high lethality. The agent was first described in 1957 and, since then, knowledge about the pathogen itself, and its interactions with the host, has increased continuously. This is, in part, due to the fact that experimental infections can be studied in the natural host. However, the fact that most commercial pigs are colonized by this pathogen has hampered the applicability of knowledge gained under experimental conditions. In addition, several factors are involved in development of disease, and these have often been studied individually. In a DISCONTOOLS initiative, members from science, industry and clinics exchanged their expertise and empirical observations and identified the major gaps in knowledge. This review sums up published results and expert opinions, within the fields of pathogenesis, epidemiology, transmission, immune response to infection, as well as the main means of prevention, detection and control. The gaps that still remain to be filled are highlighted, and present as well as future challenges in the control of this disease are addressed.

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Section: Immune Response To Infectionmentioning

confidence: 99%

Update onActinobacillus pleuropneumoniae-knowledge, gaps and challenges

Sassu

Bossé

Tobias

et al. 2017

Transbound Emerg Dis

144

178

View full text Add to dashboard Cite

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“…In adult boars, CRP reference values were determined to be 3.6–183 μg/mL and significant differences were found between different high health breeding lines (67). In slaughter pigs, Hp concentrations seem to be elevated compared to other published physiological cut-offs (68). In the present study, Hp- and CRP-values were assessed in accordance with the published cut-offs of Heegard et al (21) because these were evaluated for a non-defined status of inflammation, but also according to age-related reference values (39, 46).…”

Section: Discussionmentioning

confidence: 68%

Haptoglobin and C-Reactive Protein—Non-specific Markers for Nursery Conditions in Swine

et al. 2019

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A quality concept for production in the pork market includes granting a good health status of pigs from birth to slaughter. This concept is a precondition for animal welfare as well as reducing antibiotic usage in farm animals. The demand for fighting bacterial antimicrobial resistance in humans, animals, and in the environment is one driving force for the development of innovative technical solutions to improve husbandry. Maintenance of a good health status in pigs depends on early detection of a disturbance in homeostasis in critical phases of life. This can be measured by non-specific biomarkers as acute phase proteins. In this project, husbandry conditions and health status in nursery pigs were monitored in an autumn and winter nursery period from weaning to the end of nursery in two compartments with 180 pigs each. It was investigated whether a slight modification in indoor climate achieved by a new ammonia sensory technology coupled with the electronic control unit of the forced ventilation system ensuring ammonia levels lower than 5 ± 3 ppm in one compartment led to a better health status in piglets in comparison to the control compartment. In the examined nursery periods in different seasons, ammonia concentrations in the experimental compartment were significantly lower than in the control compartment, thus proving the functionality and efficacy of the technical system. Production parameters as feed conversion rate and average daily weight gain were slightly improved in the experimental compartment without implementing other measures. Multifactorial analysis of variance resulted in a significant influence of season, daily quarter, and compartment on ammonia concentration. The challenge to preserve a high health status of piglets also during suboptimal outside climate in the transitional season was reflected by an increase in the acute-phase proteins haptoglobin (Hp) and C-reactive protein (CRP) in autumn compared to winter. The seasonal influence on concentrations of CRP and Hp superimposed potential influences of the climate modification. New technological concepts to reduce noxious gases and dust in the animal environment as well as emissions, which in parallel guarantee optimal temperatures also during extreme weather conditions, can be evaluated by clinical data in combination with biomarkers.

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“…Data were analyzed using the inbuilt software CFX manager (Version 2.0, Bio-Rad) in the linear regression mode. Expression of target genes was calculated against IS using a modified ΔΔCq method and normalized for the relative expression values obtained for internal reference genes HPRT & Cyc as previously described ( 45 ). Values obtained from duplicates were averaged and expressed as 2 −ΔΔCq in fold changes relative to the IS.…”

Section: Methodsmentioning

confidence: 99%