Towards a better understanding of heterophile (and the like) antibody interference with modern immunoassays

Levinson, Stanley S.; Miller, James J.

doi:10.1016/s0009-8981(02)00275-9

Cited by 214 publications

(183 citation statements)

References 68 publications

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“…The binding reaction was inhibited by animal serum or the crude IgG such as dog, horse, bovine, porcine, goat, ovine, rabbit, guinea-pig, rat, mouse but not human, monkey and chick. These antibodies had crossreactivity with these animal IgGs except human IgG, as previously reported [10,11,13,14,[24][25][26]. In the present study also no cross-reaction with monkey was observed.…”

supporting

confidence: 89%

Neutralization and purification of thyroid stimulating antibody (TSAb) and thyroid blocking antibody (TBAb) by heterophilic antibody to animal IgG in Graves' disease

et al. 2012

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supporting

confidence: 89%

Neutralization and purification of thyroid stimulating antibody (TSAb) and thyroid blocking antibody (TBAb) by heterophilic antibody to animal IgG in Graves' disease

et al. 2012

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“…Preissner and co-workers reported 32 false positive or falsely increased serum Tg values from 1106 patients with serum TgG1.00 ng/mL (2%) (6). However, HAb may also bind to the capture antibody preventing the binding of analyte and/or detection antibody causing false negative or falsely low results (5). Accordingly, we observed a case of HAb-induced false negative Tg measurement in a patient with lymph-node metastases from DTC (7).…”

Section: Introductionmentioning

confidence: 63%

“…Heterophile antibodies (HAb) can bind to animal antigens or antibodies employed in immunometric assays (4). HAbs can form a bridge between the capture and detection antibody, leading to a false positive result in absence of analyte, or to a falsely higher result in presence of analyte (5). Preissner and co-workers reported 32 false positive or falsely increased serum Tg values from 1106 patients with serum TgG1.00 ng/mL (2%) (6).…”

Section: Introductionmentioning

confidence: 99%

Heterophile antibodies may falsely increase or decrease thyroglobulin measurement in patients with differentiated thyroid carcinoma

Giovanella¹,

Keller

Ceriani

et al. 2009

Clinical Chemistry and Laboratory Medicine

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Background: To examine the prevalence of significant interference from heterophile antibodies (HAb) in the measurement of serum thyroglobulin (Tg), we evaluated a large cohort of samples from patients with differentiated thyroid carcinoma (DTC). Methods: Serum Tg measurements were performed in 406 serum samples before and after incubation of each serum sample in heterophile-blocking tubes (HBT) at room temperature for 1 h. We calculated the difference between the original Tg value and the value obtained after HBT treatment. We considered any sample showing an absolute percent difference )3 SD from the mean percent difference as being affected by HAb interference. Results: We identified five patients (1%) as showing interference from HAb. Of these, three (60%) showed a false positive or falsely increased Tg concentration without any recurrence following clinical work-up; two (40%) showed a false negative or falsely reduced Tg levels, and metastases were detected in both cases by imaging procedures. Conclusions: HAb may increase as well as reduce the measured Tg in a significant number of patients. A positive HAb interference should be suspected if Tg elevation does not fit the clinical pictures. A negative interference is a more challenging problem because increases in Tg generally occur as the first sign of recurrence of DTC. Therefore, treatment using HBT tubes of all sera referred for Tg measurement should be considered in order to prevent both unwarranted investigations or therapy, and delayed diagnosis of recurrence in patients affected by DTC. Clin Chem Lab Med 2009;47:952-4.

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“…Although immunoglobulins from the animal species used to generate assay antibodies are added to bind with potential interferents, and mitigate their impact, it has also been recommended to add immunoglobulin from a second species (11 ). This makes for a complex investigation if multiple species are involved.…”

Section: Discussionmentioning

confidence: 99%

A Girl with Goiter and Inappropriate Thyroid-Stimulating Hormone Secretion

et al. 2008

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FT 4 value based on the post-PEG FT 4 value, and this correlated well with results obtained on other analyzers (Table 1). In this case, it was possible to demonstrate thyroxine-autoantibody interference in the Advia Centaur FT 4 immunoassay by PEG precipitation of serum and subsequent comparison of the difference between pre-and post-PEG FT 4 results with those of controls.The data suggest the presence of thyroid hormone autoantibodies. The patient was not treated for thyroidal illness and remained clinically euthyroid 6 months later.

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Towards a better understanding of heterophile (and the like) antibody interference with modern immunoassays

Cited by 214 publications

References 68 publications

Neutralization and purification of thyroid stimulating antibody (TSAb) and thyroid blocking antibody (TBAb) by heterophilic antibody to animal IgG in Graves' disease

Neutralization and purification of thyroid stimulating antibody (TSAb) and thyroid blocking antibody (TBAb) by heterophilic antibody to animal IgG in Graves' disease

Heterophile antibodies may falsely increase or decrease thyroglobulin measurement in patients with differentiated thyroid carcinoma

A Girl with Goiter and Inappropriate Thyroid-Stimulating Hormone Secretion

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