Toward whole tissue imaging of axolotl regeneration

Masselink, Wouter; Tanaka, Elly M.

doi:10.1002/dvdy.282

Cited by 13 publications

(11 citation statements)

References 47 publications

(98 reference statements)

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“…Modelling axolotl joint growth using our approach is also feasible. The axolotl is often used as a model for limb development (Nye et al 2003, Hutchison et al 2007) and progress has been made in visualising cells at high resolution during live imaging (Masselink and Tanaka 2021). The existence of rainbow transgenic lines also facilitates cell tracking and visualisation and was used in the past to study digit tip regeneration (Currie et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Growth orientations, rather than heterogeneous growth rates, dominate jaw joint morphogenesis in the larval zebrafish

Godivier

Lawrence

Wang

et al. 2022

Preprint

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In early limb embryogenesis, synovial joints acquire specific shapes which determine joint motion and function. The process by which the opposing cartilaginous joint surfaces are moulded into reciprocal and interlocking shapes, called joint morphogenesis, is one of the least understood aspect of joint formation and the cell-level dynamics underlying it are yet to be unravelled. In this research, we quantified key cellular dynamics involved in growth and morphogenesis of the zebrafish jaw joint and synthesised them in a predictive computational simulation of joint development. Cells in larval zebrafish jaw joints labelled with cartilage markers were tracked over a forty-eight hour time window using confocal imaging. Changes in distance and angle between adjacent cell centroids resulting from cell rearrangement, volume expansion and extracellular matrix (ECM) deposition were measured and used to calculate the rate and direction of local tissue deformations. We observed spatially and temporally heterogeneous growth patterns with marked anisotropy over the developmental period assessed. There was notably elevated growth at the level of the retroarticular process of the Meckel's cartilage, a feature known to undergo pronounced shape changes during zebrafish development. Analysis of cell dynamics indicated a dominant role for cell volume expansion in growth, with minor influences from ECM volume increases and cell intercalation. Cell proliferation in the joint was minimal over the timeframe of interest. Synthesising the dynamic cell data into a finite element model of jaw joint development resulted in accurate shape predictions. Our biofidelic computational simulation demonstrated that zebrafish jaw joint growth can be reasonably approximated based on cell positional information over time, where cell positional information derives mainly from cell orientation and cell volume expansion. By modifying the input parameters of the simulation, we were able to assess the relative contributions of heterogeneous growth rates and of growth orientation. The use of uniform rather than heterogeneous growth rates only minorly impacted the shape predictions whereas isotropic growth fields resulted in altered shape predictions. The simulation results suggest that growth anisotropy is the dominant influence on joint growth and morphogenesis. This study addresses the gap of the cellular processes underlying joint morphogenesis, with implications for understanding the aetiology of developmental joint disorders such as developmental dysplasia of the hip and arthrogryposis.

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Section: Discussionmentioning

confidence: 99%

Growth orientations, rather than heterogeneous growth rates, dominate jaw joint morphogenesis in the larval zebrafish

Godivier

Lawrence

Wang

et al. 2022

Preprint

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“…However, successful strategies for tackling stubborn transgenesis or imaging in one model inform approaches for how to solve similar problems in subsequent models. For example, both axolotls and Xenopus contain the same three types of pigment as zebrafish ( Masselink and Tanaka, 2021 ). Indeed, albino strains of both Xenopus and Axolotl already exist, but retain some pigmentation ( Frost and Malacinski, 1979 ; Woodcock et al, 2017 ; Fukuzawa, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

A Model of Discovery: The Role of Imaging Established and Emerging Non-mammalian Models in Neuroscience

Haynes

Ulland

Eliceiri

2022

Front. Mol. Neurosci.

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Rodents have been the dominant animal models in neurobiology and neurological disease research over the past 60 years. The prevalent use of rats and mice in neuroscience research has been driven by several key attributes including their organ physiology being more similar to humans, the availability of a broad variety of behavioral tests and genetic tools, and widely accessible reagents. However, despite the many advances in understanding neurobiology that have been achieved using rodent models, there remain key limitations in the questions that can be addressed in these and other mammalian models. In particular, in vivo imaging in mammals at the cell-resolution level remains technically difficult and demands large investments in time and cost. The simpler nervous systems of many non-mammalian models allow for precise mapping of circuits and even the whole brain with impressive subcellular resolution. The types of non-mammalian neuroscience models available spans vertebrates and non-vertebrates, so that an appropriate model for most cell biological questions in neurodegenerative disease likely exists. A push to diversify the models used in neuroscience research could help address current gaps in knowledge, complement existing rodent-based bodies of work, and bring new insight into our understanding of human disease. Moreover, there are inherent aspects of many non-mammalian models such as lifespan and tissue transparency that can make them specifically advantageous for neuroscience studies. Crispr/Cas9 gene editing and decreased cost of genome sequencing combined with advances in optical microscopy enhances the utility of new animal models to address specific questions. This review seeks to synthesize current knowledge of established and emerging non-mammalian model organisms with advances in cellular-resolution in vivo imaging techniques to suggest new approaches to understand neurodegeneration and neurobiological processes. We will summarize current tools and in vivo imaging approaches at the single cell scale that could help lead to increased consideration of non-mammalian models in neuroscience research.

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“…As the evolutionarily closest organisms to humans capable of complex regeneration, salamanders constitute valuable models for regenerative biology studies. In particular, the axolotl— Ambystoma mexicanum— and the Iberian ribbed newt— Pleurodeles waltl— are two laboratory-tractable systems whose adoption has exponentially grown in recent years due to the ease of captive breeding and rearing ( Khattak et al, 2014 ; Joven et al, 2015 ), efficient transgenesis and genome editing methods ( Khattak et al, 2013b ; Hayashi et al, 2014 ; Fei et al, 2018 ; Cai et al, 2019 ), availability of genomic and transcriptomic information ( Elewa et al, 2017 ; Nowoshilow et al, 2018 ; Smith et al, 2019 ), and advanced imaging techniques ( Masselink and Tanaka, 2020 ; Subiran Adrados et al, 2020 ; Box 1 ). Newts present a conventional salamander life cycle, undergoing metamorphosis and becoming fully developed adults with reduced to imperceptible continuous growth.…”

Section: Introductionmentioning

confidence: 99%

“…Many salamander tissues are optically transparent, and highly suited to live imaging. Further, several optical clearing methods have been adapted to the salamander system, enabling volumetric quantitative imaging ( Duerr et al, 2020 ; Masselink and Tanaka, 2020 ; Pinheiro et al, 2020 ; Subiran Adrados et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Salamander Insights Into Ageing and Rejuvenation

Yun

2021

Front. Cell Dev. Biol.

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Exhibiting extreme regenerative abilities which extend to complex organs and entire limbs, salamanders have long served as research models for understanding the basis of vertebrate regeneration. Yet these organisms display additional noteworthy traits, namely extraordinary longevity, indefinite regenerative potential and apparent lack of traditional signs of age-related decay or “negligible senescence.” Here, I examine existing studies addressing these features, highlight outstanding questions, and argue that salamanders constitute valuable models for addressing the nature of organismal senescence and the interplay between regeneration and ageing.

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Toward whole tissue imaging of axolotl regeneration

Cited by 13 publications

References 47 publications

Growth orientations, rather than heterogeneous growth rates, dominate jaw joint morphogenesis in the larval zebrafish

Growth orientations, rather than heterogeneous growth rates, dominate jaw joint morphogenesis in the larval zebrafish

A Model of Discovery: The Role of Imaging Established and Emerging Non-mammalian Models in Neuroscience

Salamander Insights Into Ageing and Rejuvenation

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