Toward Visualizing Genomic DNA Using Electron Microscopy via DNA Metallization

Jin, Xuelin; Kannappan, Shrute; Hapsari, Natalia Diyah; Jin, Yu; Kim, Kyeong Kyu; Lee, Jung Heon; Jo, Kyubong

doi:10.1002/sstr.202200361

Cited by 5 publications

(2 citation statements)

References 198 publications

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“…However, implementing this method is challenging in carbon-film-based TEM imaging of DNA molecules. As a result, most TEM DNA images documented to date exhibit random arrangements, [21] similar to those observed in Figure 1a.…”

Section: Sem Imaging Of Dna With Novel Electro-stainsupporting

confidence: 61%

Scanning Electron Microscopy Imaging of Large DNA Molecules Using a Metal‐Free Electro‐Stain Composed of DNA‐Binding Proteins and Synthetic Polymers

Noh,

Kang,

Heo

et al. 2024

Advanced Science

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This paper presents the first scanning electron microscopy (SEM)‐based DNA imaging in biological samples. This novel approach incorporates a metal‐free electro‐stain reagent, formulated by combining DNA‐binding proteins and synthetic polymers to enhance the visibility of 2‐nm‐thick DNA under SEM. Notably, DNA molecules stain with proteins and polymers appear as dark lines under SEM. The resulting DNA images exhibit a thickness of 15.0±4.0 nm. As SEM is the primary platform, it integrates seamlessly with various chemically functionalized large surfaces with the aid of microfluidic devices. The approach allows high‐resolution imaging of various DNA structures including linear, circular, single‐stranded DNA and RNA, originating from nuclear and mitochondrial genomes. Furthermore, quantum dots are successfully visualized as bright labels that are sequence‐specifically incorporated into DNA molecules, which highlights the potential for SEM‐based optical DNA mapping. In conclusion, DNA imaging using SEM with the novel electro‐stain offers electron microscopic resolution with the ease of optical microscopy.

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Section: Sem Imaging Of Dna With Novel Electro-stainsupporting

confidence: 61%

Scanning Electron Microscopy Imaging of Large DNA Molecules Using a Metal‐Free Electro‐Stain Composed of DNA‐Binding Proteins and Synthetic Polymers

Noh,

Kang,

Heo

et al. 2024

Advanced Science

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“…243 It serves as a blueprint for life and provides instructions for the development and functioning of all living organisms. 244,245 The DNA molecule comprises two complementary strands that run in opposite directions and are held together by H bonds between nitrogenous bases. It consists of repeating units of four nitrogenous bases: adenine (A), guanine (G), cytosine (C), and thymine (T), and a sugar-phosphate backbone.…”

Section: Deoxyribose Nucleic Acid (Dna)-inspired Moleculesmentioning

confidence: 99%

Multifunctional small biomolecules as key building blocks in the development of hydrogel-based strain sensors

Zaidi¹,

Saeed²,

Heo³

et al. 2023

J. Mater. Chem. A

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DNA‐Based Conductors: From Materials Design to Ultra‐Scaled Electronics

Wang,

Deng,

Lin

et al. 2024

Small Methods

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Photolithography has been the foundational fabrication paradigm in current high‐performance electronics. However, due to the limitation in fabrication resolution, scaling beyond a 20‐nm critical dimension for metal conductors presents a significant challenge for photolithography. Structural DNA nanotechnology emerges as a promising alternative to photolithography, allowing for the site‐specific assembly of nano‐materials at single‐molecule resolution. Substantial progresses have been achieved in the ultra‐scaled DNA‐based conductors, exhibiting novel transport characteristics and small critical dimensions. This review highlights the structure‐transport property relationship for various DNA‐based conductors and their potential applications in quantum /semiconductor electronics, going beyond the conventional scope focusing mainly on the shape diversity of DNA‐templated metals. Different material synthesis methods and their morphological impacts on the conductivities are discussed in detail, with particular emphasis on the conducting mechanisms, such as insulating, metallic conducting, quantum tunneling, and superconducting. Furthermore, the ionic gating effect of self‐assembled DNA structures in electrolyte solutions is examined. This review also suggests potential solutions to address current challenges in DNA‐based conductors, encouraging multi‐disciplinary collaborations for the future development of this exciting area.

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Toward Visualizing Genomic DNA Using Electron Microscopy via DNA Metallization

Cited by 5 publications

References 198 publications

Scanning Electron Microscopy Imaging of Large DNA Molecules Using a Metal‐Free Electro‐Stain Composed of DNA‐Binding Proteins and Synthetic Polymers

Scanning Electron Microscopy Imaging of Large DNA Molecules Using a Metal‐Free Electro‐Stain Composed of DNA‐Binding Proteins and Synthetic Polymers

Multifunctional small biomolecules as key building blocks in the development of hydrogel-based strain sensors

DNA‐Based Conductors: From Materials Design to Ultra‐Scaled Electronics

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