Toward Using Fluorescent Nanodiamonds To Study Chronological Aging in <i>Saccharomyces cerevisiae</i>

Laan, Kiran J. van der; Naulleau, Julie; Damle, Viraj; Sigaeva, Alina; Jamot, Nicolas; Perona-Martinez, Felipe P; Chipaux, Mayeul; Schirhagl, Romana

doi:10.1021/acs.analchem.8b03431

Cited by 21 publications

(25 citation statements)

References 33 publications

(61 reference statements)

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“…Here we have shown that FNDs, in the longer term, do not interfere with this YAP1-mediated oxidative stress defense during chronological aging. This corresponds to the earlier reporting of no significant differences in the survival of a chronological aging cell population after treatment with CT and FNDs [13].…”

Section: Transient Fnd-induced Changes In Oxidative Stress Transcriptomesupporting

confidence: 91%

“…In previous publications [13,16], we have shown that cells survived the presence of FND. In this paper we take biocompatibility one step further.…”

Section: Resultsmentioning

confidence: 64%

“…Yeast is a well-known cellular model to study aging. In earlier studies, the proliferation capacity and the aging curves of the cells were shown to remain unaffected after the introduction of FNDs [13]. However, there are other ways of assessing the cell viability and evaluating the response of cells.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the Oxidative Stress Response of Aging Yeast Cells in Response to Internalization of Fluorescent Nanodiamond Biosensors

et al. 2020

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Fluorescent nanodiamonds (FNDs) are proposed to be used as free radical biosensors, as they function as magnetic sensors, changing their optical properties depending on their magnetic surroundings. Free radicals are produced during natural cell metabolism, but when the natural balance is disturbed, they are also associated with diseases and aging. Sensitive methods to detect free radicals are challenging, due to their high reactivity and transiency, providing the need for new biosensors such as FNDs. Here we have studied in detail the stress response of an aging model system, yeast cells, upon FND internalization to assess whether one can safely use this biosensor in the desired model. This was done by measuring metabolic activity, the activity of genes involved in different steps and the locations of the oxidative stress defense systems and general free radical activity. Only minimal, transient FND-related stress effects were observed, highlighting excellent biocompatibility in the long term. This is a crucial milestone towards the applicability of FNDs as biosensors in free radical research.

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Section: Transient Fnd-induced Changes In Oxidative Stress Transcriptomesupporting

confidence: 91%

“…In previous publications [13,16], we have shown that cells survived the presence of FND. In this paper we take biocompatibility one step further.…”

Section: Resultsmentioning

confidence: 64%

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Evaluation of the Oxidative Stress Response of Aging Yeast Cells in Response to Internalization of Fluorescent Nanodiamond Biosensors

et al. 2020

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“…Thus, we surmise that for both particle types a large proportion of diamond particles is excreted from the cells. Excretion in yeast has been observed for diamond [17] as well as for other materials. The process has been described to happen when the cells are not in a balanced condition.…”

Section: Fnd Quantification After Cell Divisionmentioning

confidence: 94%

“…To achieve uptake, two protocols have been established: One option is to permeabilize the cell wall, which allows the diamond particles to enter. It has also been shown in a previous work that the cells could proliferate after being treated with FNDs [16,17]. Another option, which we used here, is to remove the cell wall entirely.…”

Section: Introductionmentioning

confidence: 97%

The Fate of Lipid-Coated and Uncoated Fluorescent Nanodiamonds during Cell Division in Yeast

et al. 2020

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Fluorescent nanodiamonds are frequently used as biolabels. They have also recently been established for magnetic resonance and temperature sensing at the nanoscale level. To properly use them in cell biology, we first have to understand their intracellular fate. Here, we investigated, for the first time, what happens to diamond particles during and after cell division in yeast (Saccharomyces cerevisiae) cells. More concretely, our goal was to answer the question of whether nanodiamonds remain in the mother cells or end up in the daughter cells. Yeast cells are widely used as a model organism in aging and biotechnology research, and they are particularly interesting because their asymmetric cell division leads to morphologically different mother and daughter cells. Although yeast cells have a mechanism to prevent potentially harmful substances from entering the daughter cells, we found an increased number of diamond particles in daughter cells. Additionally, we found substantial excretion of particles, which has not been reported for mammalian cells. We also investigated what types of movement diamond particles undergo in the cells. Finally, we also compared bare nanodiamonds with lipid-coated diamonds, and there were no significant differences in respect to either movement or intracellular fate.

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Applications of Fluorescent Nanodiamond in Biology

Hsiao¹,

Le²,

Chang³

2022

Encyclopedia of Analytical Chemistry

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Fluorescent nanodiamond (FND) has emerged as a promising material in several multidisciplinary areas, including biology, chemistry, physics, and materials science. Composed of sp 3 ‐carbon atoms, FND offers superior biocompatibility, chemical inertness, a large surface area, tunable surface structure, and excellent mechanical characteristics. The nanoparticle is unique in that it comprises a high‐density ensemble of negatively charged nitrogen‐vacancy (NV − ) centers that act as built‐in fluorophores and exhibit a number of remarkable optical and magnetic properties. These properties make FND particularly well suited for a wide range of applications, including cell labeling, long‐term cell tracking, super‐resolution imaging, nanoscale sensing, and drug delivery. This article discusses recent applications of FND‐enabled developments in biology.

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Toward Using Fluorescent Nanodiamonds To Study Chronological Aging in Saccharomyces cerevisiae

Cited by 21 publications

References 33 publications

Evaluation of the Oxidative Stress Response of Aging Yeast Cells in Response to Internalization of Fluorescent Nanodiamond Biosensors

Evaluation of the Oxidative Stress Response of Aging Yeast Cells in Response to Internalization of Fluorescent Nanodiamond Biosensors

The Fate of Lipid-Coated and Uncoated Fluorescent Nanodiamonds during Cell Division in Yeast

Applications of Fluorescent Nanodiamond in Biology

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