Toward Understanding Bacterial Ice Nucleation

Lukas, Max; Schwidetzky, Ralph; Eufemio, Rosemary J; Bonn, Mischa; Meister, Konrad

doi:10.1021/acs.jpcb.1c09342

Cited by 37 publications

(49 citation statements)

References 57 publications

(140 reference statements)

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“…Then followed less powerful but much more abundant inorganic ice nucleators such as metals [ 25 ] and metal oxides [ 33 ], along with some organic substances [ 34 , 35 , 36 ] and biological objects such as pollen and bacteria [ 37 , 38 , 39 , 40 ]. For our experiments, we chose two ice nucleators that are very different in chemical nature: the inorganic compound CuO (in the form of powder), and the bacterium Pseudomonas syringae , which now seems to be the most powerful nucleator of ice [ 38 , 39 , 41 , 42 ].…”

Section: A Brief Overview Of Experiments On Ice Nucleation At High Su...mentioning

confidence: 99%

How Can Ice Emerge at 0 °C?

2022

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The classical nucleation theory shows that bulk water freezing does not occur at temperatures above −30 °C, and that at higher temperatures ice nucleation requires the presence of some ice-binding surfaces. The temperature and rate of ice nucleation depend on the size and level of complementarity between the atomic structure of these surfaces and various H-bond-rich/depleted crystal planes. In our experiments, the ice nucleation temperature was within a range from −8 °C to −15 °C for buffer and water in plastic test tubes. Upon the addition of ice-initiating substances (i.e., conventional AgI or CuO investigated here), ice appeared in a range from −3 °C to −7 °C, and in the presence of the ice-nucleating bacterium Pseudomonas syringae from −1 °C to −2 °C. The addition of an antifreeze protein inhibited the action of the tested ice-initiating agents.

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Section: A Brief Overview Of Experiments On Ice Nucleation At High Su...mentioning

confidence: 99%

How Can Ice Emerge at 0 °C?

2022

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“…These differences resulted in the dissimilar size of functional assembly at the bacterial outer cell membrane that arrange water molecules into an “ice-like” structure and nucleate ice formation. Large functional assembly of Class A INA bacteria is more effective at freezing water at a warmer temperature than the small functional assembly of Class C INA [ 39 ]. The Class C of INA bacteria may hold some of the previously unreported genus or species that has INA properties, such as Stenotrophomonas and Gram-positive Lysinibacillus collected from Virginia which were firstly reported to be able to nucleate ice at -8 °C [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

Prevalence and characterization of Ice Nucleation Active (INA) bacteria from rainwater in Indonesia

et al. 2022

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Background Ice nucleation active (INA) bacteria are a group of microorganisms that can act as biological nucleator due to their ice nucleation protein property. Unfortunately, little is known about their prevalence and characteristics in tropical areas including Indonesia. Here, we monitor the presence of INA bacteria in rainwater and air samples collected from Jakarta, Tangerang and several areas in Western Java, Indonesia for one year. We further identify and characterize selected Class A of INA bacteria isolated from these areas. Results Most of the INA bacteria were isolated from rainwater samples collected during March–August 2010, particularly from Jakarta, Bandung, and Tangerang. A total of 1,902 bacterial isolates were recovered from these area. We found a limited number of bacterial isolates from air sampling. From ice nucleation activity assays, 101 INA isolates were found active as ice nucleator at a temperature above -10 °C. A large majority (73 isolates) of them are classified as Class C (active below -8 °C), followed by Class A (26 isolates; active at -2 to -5 °C) and Class B (two isolates; active at -5 to -8 °C). We sequenced the 16S rRNA gene of 18 Class A INA isolates and identified 15 isolates as Enterobacteriaceae, while the remaining three as Pseudomonadaceae. The vast majority of our Class A INA isolates were likely Pantoea spp. with several isolates were deduced as either Pseudomonas, Cronobacter, and Klebsiella. We found that these 18 Class A INA isolates had acquired resistance to antibiotics erythromycin and ampicillin, which are considered two critically important antibiotics. Conclusions Our results showed that the prevalence of INA bacterial population varies across locations and seasons. Furthermore, our isolates were dominated by Class A and C INA bacteria. This study also cautions regarding the spread of antibiotic resistance among INA bacteria.

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“…All three species are home to microbes with ice + potential, including Pseudomonas syringae , P. fluorescens , Mortierella spp., and Fusarium spp. [ 103 ]. Any combination of these taxa could contribute to freeze-tolerance of the insect hosts.…”

Section: Exploring the Microbiomementioning

confidence: 99%

Insect Freeze-Tolerance Downunder: The Microbial Connection

Morgan‐Richards

Marshall

Biggs

et al. 2023

Insects

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Insects that are freeze-tolerant start freezing at high sub-zero temperatures and produce small ice crystals. They do this using ice-nucleating agents that facilitate intercellular ice growth and prevent formation of large crystals where they can damage tissues. In Aotearoa/New Zealand the majority of cold adapted invertebrates studied survive freezing at any time of year, with ice formation beginning in the rich microbiome of the gut. Some freeze-tolerant insects are known to host symbiotic bacteria and/or fungi that produce ice-nucleating agents and we speculate that gut microbes of many New Zealand insects may provide ice-nucleating active compounds that moderate freezing. We consider too the possibility that evolutionary disparate freeze-tolerant insect species share gut microbes that are a source of ice-nucleating agents and so we describe potential transmission pathways of shared gut fauna. Despite more than 30 years of research into the freeze-tolerant mechanisms of Southern Hemisphere insects, the role of exogenous ice-nucleating agents has been neglected. Key traits of three New Zealand freeze-tolerant lineages are considered in light of the supercooling point (temperature of ice crystal formation) of microbial ice-nucleating particles, the initiation site of freezing, and the implications for invertebrate parasites. We outline approaches that could be used to investigate potential sources of ice-nucleating agents in freeze-tolerant insects and the tools employed to study insect microbiomes.

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Toward Understanding Bacterial Ice Nucleation

Cited by 37 publications

References 57 publications

How Can Ice Emerge at 0 °C?

How Can Ice Emerge at 0 °C?

Prevalence and characterization of Ice Nucleation Active (INA) bacteria from rainwater in Indonesia

Insect Freeze-Tolerance Downunder: The Microbial Connection

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