Toward tissue-engineering of nasal cartilages

Lavernia, Laura; Brown, Wendy E.; Wong, Brian J. F.; Hu, Jerry C.; Athanasiou, Kyriacos A.

doi:10.1016/j.actbio.2019.02.025

Cited by 50 publications

(58 citation statements)

References 134 publications

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“…This knowledge is essential for cartilage tissue engineering and the choice of optimal biomatrices. Natural ECM compounds have the advantage that they resemble native cartilage tissue and therefore provide the natural ECM properties, also known as biomimicry 9 . A method to produce a natural ECM bioscaffold requires a decellularization and sterilization process of autologous, allogenic, or xenogenic cartilage that maintains the specific, three‐dimensional collagen structure and additionally increases the matrix porosity, thereby it favors cell migration and chondrogenic differentiation 10 .…”

Section: Introductionmentioning

confidence: 99%

Experimental approach to nasal septal cartilage regeneration with adipose tissue‐derived stem cells and decellularized porcine septal cartilage

Kuhlmann¹,

Schenck²,

Tluczynski

et al. 2020

Xenotransplantation

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Background Cartilage shortage is a major problem in facial reconstructive surgery. Prior studies have shown that decellularized porcine nasal septal cartilage (DPNC) seeded with primary human nasal chondrocytes enabled cartilage regeneration and showed potential as a replacement material for nasal cartilage. Since adipose tissue‐derived stem cells (ASCs) are easily accessible and almost abundantly available, they appear to be a promising alternative to limited chondrocytes making the combination of DPNC and ASCs a feasible approach towards clinical translation. Thus, this study was intended to investigate the interactions between ASCs and DPNC in an in vitro model. Methods DPNCs were seeded and 3D‐cultured with primary human ASCs that were priorly characterized with trilineage differentiation and flow cytometry. Cell vitality and proliferation were evaluated by Live‐Dead, alamarBlue, and PicoGreen assays. Chondrogenic differentiation was examined by DMMB assay and cryosectioning‐based histology. Cell invasion within DPNC was visualized and quantified by fluorescent histology (DAPI, Phalloidin). Results ASCs showed good adherence to DPNC and Live‐Dead assay proved their viability over 2 weeks. AlamarBlueassay showed an increase in metabolic activity compared to 2D cultures, and PicoGreen assay demonstrated an increase of cell number within DPNC over time. Biochemical assays and histology added evidence of chondrogenic differentiation of 3D‐cultured ASCs under the influence of chondrogenic induction medium. Fluorescent image analysis showed a significant increase of cell‐occupied areas of scaffolds over time (P < .05). Conclusions DPNC scaffolds provided a suitable environment for ASCs that allowed good cell vitality, high proliferation, and chondrogenic differentiation. Thus, the use of ASCs and DPNC yields a promising alternative to the use of primary human chondrocytes. For facial cartilage tissue engineering, we regard ASCs as an attractive alternative to human nasal chondrocytes due to their better accessibility and availability. Further research will be necessary to determine long‐term effects and in vivo outcomes of ASCs and DPNC in cartilage regeneration of the face.

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Section: Introductionmentioning

confidence: 99%

Experimental approach to nasal septal cartilage regeneration with adipose tissue‐derived stem cells and decellularized porcine septal cartilage

Kuhlmann¹,

Schenck²,

Tluczynski

et al. 2020

Xenotransplantation

View full text Add to dashboard Cite

show abstract

“…With simulations alone, discussing the association between the outcomes and the real situation will always be inconclusive, and the results will be limited to theory forever. When applying finite element analysis for surgery, it is recommended to perform the study as follows: (1) determine the clinical problems and design the modifications or solutions; (2) use computational simulations to predict the changes, compare the results to the objectives, and change the solution if the results do not meet the expectations; (3) practice the solutions to the clinical work and collect the data; and (4) compare the clinical outcomes with the computational results to finally decide if the problems have been solved. This kind of combination of theory and practice should help to impact this computer-simulating clinical research area.…”

Section: Finite Element Methodsmentioning

confidence: 99%

“…In recent years, biomedical engineering applications for the nasal cartilage has developed rapidly. 1,2 Computational technology is one aspect of biomedical engineering that is used in clinical research, which forms the link between principle and practice. Computational technology can assist in exploring the role of physical factors and predict the results to guide the external conditions that should be applied in practice.…”

Section: Introductionmentioning

confidence: 99%

Computational technology for nasal cartilage-related clinical research and application

Shi

Huang

2020

Int J Oral Sci

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Surgeons need to understand the effects of the nasal cartilage on facial morphology, the function of both soft tissues and hard tissues and nasal function when performing nasal surgery. In nasal cartilage-related surgery, the main goals for clinical research should include clarification of surgical goals, rationalization of surgical methods, precision and personalization of surgical design and preparation and improved convenience of doctor-patient communication. Computational technology has become an effective way to achieve these goals. Advances in three-dimensional (3D) imaging technology will promote nasal cartilage-related applications, including research on computational modelling technology, computational simulation technology, virtual surgery planning and 3D printing technology. These technologies are destined to revolutionize nasal surgery further. In this review, we summarize the advantages, latest findings and application progress of various computational technologies used in clinical nasal cartilage-related work and research. The application prospects of each technique are also discussed.

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“…The basic concepts of tissue engineering consist of cells, scaffolds, and stimuli [4], although in some cases, cells are the only component required to form functional neocartilage [5]. Generally, the cartilage tissue engineering process includes the following steps: harvesting of autologous chondrogenic cells, cell expansion under a monolayer culture, redifferentiation under 3D culture, in vitro incubation with a scaffold, and transfer to patients ( Fig.…”

Section: Introductionmentioning

confidence: 99%

Cartilage tissue engineering for craniofacial reconstruction

Kim

2020

Arch Plast Surg

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Severe cartilage defects and congenital anomalies affect millions of people and involve considerable medical expenses. Tissue engineering offers many advantages over conventional treatments, as therapy can be tailored to specific defects using abundant bioengineered resources. This article introduces the basic concepts of cartilage tissue engineering and reviews recent progress in the field, with a focus on craniofacial reconstruction and facial aesthetics. The basic concepts of tissue engineering consist of cells, scaffolds, and stimuli. Generally, the cartilage tissue engineering process includes the following steps: harvesting autologous chondrogenic cells, cell expansion, redifferentiation, <i>in vitro</i> incubation with a scaffold, and transfer to patients. Despite the promising prospects of cartilage tissue engineering, problems and challenges still exist due to certain limitations. The limited proliferation of chondrocytes and their tendency to dedifferentiate necessitate further developments in stem cell technology and chondrocyte molecular biology. Progress should be made in designing fully biocompatible scaffolds with a minimal immune response to regenerate tissue effectively.

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Toward tissue-engineering of nasal cartilages

Cited by 50 publications

References 134 publications

Experimental approach to nasal septal cartilage regeneration with adipose tissue‐derived stem cells and decellularized porcine septal cartilage

Experimental approach to nasal septal cartilage regeneration with adipose tissue‐derived stem cells and decellularized porcine septal cartilage

Computational technology for nasal cartilage-related clinical research and application

Cartilage tissue engineering for craniofacial reconstruction

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