Abstract:The actual gap of the label-free quantitative phase microscopy in respect to fluorescence microscopy, that allows the subcellular characterization by using exogenous markers, is the lack of intracellular specificity. Recently, computational methods based on artificial intelligence have been demonstrated, which allow a virtual staining of single cells in both 2D and 3D, but they require co-registration systems able to collect simultaneously both fluorescence and quantitative phase information. However, a real l… Show more
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