Toward the Identification of Mesenchymal Stem Cells in Bone Marrow and Peripheral Blood for Bone Regeneration

Smiler, Dennis; Soltan, Muna; Albitar, Mäher

doi:10.1097/id.0b013e3181835b13

Cited by 39 publications

(40 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Altogether these factors make flow cytometry an attractive method for the quantification of rare events. This technique has become a method of choice for the detection of minimal residual disease in hematological malignant neoplasms 17,19,23,24 and, more recently, an important tool in quantifying other rare events such as circulating endothelial cells, circulating progenitor cells, 19,25 mesenchymal stem cells 25 and disseminated tumor cells from CRC, 26 small and non-small lung cancer, 26 prostate cancer 27 and rhabdomyosarcoma. 28 In this study, we used an anti-pancytokeratin monoclonal antibody able to recognize an epitope present in cytokeratin 5, 6, 8 and 17; and anti-CD45 monoclonal antibody.…”

Section: Flow Cytometry In the Detection Of Disseminated Colorectal Cmentioning

confidence: 99%

Perioperative tumor cell dissemination in patients with primary or metastatic colorectal cancer

Tralhão

Hoti

Serôdio

et al. 2010

European Journal of Surgical Oncology (EJSO)

View full text Add to dashboard Cite

Introduction: Although there is general correlation between the TNM stage of colorectal cancer (CRC) and its prognosis, there is often significant variability of tumor behaviour and individual patient outcome, which is unaccounted for by pathologic factors alone. Our aim was to estimate perioperative tumor cell dissemination in patients with primary or CRC liver metastases as a possible factor influencing the outcome. Methods: Forty patients were prospectively enrolled in the study from the year 2007 to 2008. Eighteen patients had histologically proven CRC (50% rectal, 44% colonic, 6% colonic and rectal). Sixteen patients (47%) had CRC liver metastases only. The remaining six patients who underwent colon or liver resection for benign conditions, acted as the control group. All patients with malignant pathologies had R0 resections. Blood samples were taken before the surgical incision (T0), immediately after tumor resection (T1) and at the end of the surgical intervention (T2). Data acquisition was performed using a dual-laser FACSCalibur flow cytometer. Circulating malignant cells were identified as being CD45À/cytokeratinþ. Results: The analysis of patients overall (CRC resection subgroup and hepatectomy subgroup) revealed that there was no statistically significant difference of the tumoral cell count in the blood per million of hematopoietic cells at T0, T1 and T2. Conclusions: This study demonstrates no differences in the detected circulating numbers of tumor cells at different stages of surgical intervention.

show abstract

Section: Flow Cytometry In the Detection Of Disseminated Colorectal Cmentioning

confidence: 99%

Perioperative tumor cell dissemination in patients with primary or metastatic colorectal cancer

Tralhão

Hoti

Serôdio

et al. 2010

European Journal of Surgical Oncology (EJSO)

View full text Add to dashboard Cite

show abstract

“…Because a forward scatter threshold is commonly used to eliminate small particles including non-adherent platelets from flow cytometric analysis, the effect of platelet concentration on monocyte CD36 fluorescence is easily overlooked. For example, several recently published studies in patients with kidney disease (14), HIV infection (15), blood disorders (16), diabetes (6), heart disease (13), or other conditions (17) have reported quantitative expression monocyte CD36 as measured by flow cytometry using whole blood assays but without regard for the concentration of platelets in the sample. For each of these studies, we expect that variation in platelet concentrations may have substantially affected the reported results.…”

Section: Discussionmentioning

confidence: 99%

When monocytes and platelets compete: The effect of platelet count on the flow cytometric measurement of monocyte CD36

Dzik

Cserti‐Gazdewich

Ssewanyana

et al. 2009

Cytometry Part B Clinical

View full text Add to dashboard Cite

Background: Flow cytometric measurement of monocyte surface CD36 is relevant to several conditions including diabetes, cardiovascular disease, lipid disorders, platelet isoimmunization, and susceptibility to P falciparum malaria. CD36 is also strongly expressed on platelets where it is also known as platelet glycoprotein IV.Methods: Whole blood samples, containing identical monocyte concentrations, were adjusted to contain platelets ranging from 20,000/uL to 600,000/uL, were stained with fluorescent-labeled anti-CD36, and analyzed by flow cytometry.Results: CD36 median fluorescent intensity (MFI) observed on monocytes decreased as the platelet concentration in the sample increased with more than a 50% decline in monocyte MFI over the normal range of platelet values. The effect was not abolished by using larger volumes of monoclonal antibody and was observed with different clones of reagent anti-CD36. The findings were most consistent with competition by platelets for the CD36 reagent. Similar findings were observed with antibody to class I HLA. Under defined assay conditions, monocyte CD36 MFI declined with rising platelet concentration in a predictable fashion following an inverse linear relationship.Conclusions: Measurement of CD36 expression on monocytes by flow cytometry in whole blood samples is affected by the sample platelet count. When comparing the monocyte CD36 expression among different individuals, our approach can be used to adjust measured monocyte CD36 expression for the effect of the platelet concentration in the sample. Competition by platelets for monoclonal reagents may occur in other settings when whole blood assays are used and when the target antigen is strongly expressed on both platelets and leukocytes.

show abstract

“…Hence, perichondral progenitor cells could proliferate and regenerate new cartilage more vigorously than the bone marrow-derived equivalent. Although MSC in peripheral blood and connective stromal tissue may be theoretically possible sources of MSC in this experiment, perichondrium is a far larger and more efficient reservoir of progenitor cells for osteochondral tissue regeneration [Park et al, 2006;Smiler et al, 2008]. The regeneration of osteochondral tissue in the perichondrium-preserved group mainly comes from the progenitor cells in the perichondrium.…”

Section: Discussionmentioning

confidence: 99%

Long-Term Histologic Changes and Effects of Perichondrium Preservation of Auricular Cartilage Grafted in Rabbit Tympanic Bullae

2012

Audiol Neurotol

View full text Add to dashboard Cite

Introduction: This study evaluated the long-term histologic changes of grafted auricular cartilage in rabbit tympanic bullae. Materials and Methods: Auricular cartilage with or without perichondrium was prepared and cut into small pieces to obliterate the rabbit tympanic bullae. The histologic changes of the grafted cartilage in both groups were compared 20 months after surgery. Results: Remarkable spongy bony trabeculae of mature lamellar bone with red bone marrow formation were observed in the perichondrium-preserved group. Parts of the grafted cartilage pieces were invaded and replaced by bone and bone marrow. The grafted cartilage pieces grossly maintained their original polygonal shapes, and no osteochondral tissue regeneration was observed in the perichondrium-removed group. The viable chondrocyte ratios were 46.21 ± 5.58 versus 27.80 ± 4.81%, and the minimal resorption ratios were 10.31 ± 3.27 versus 2.98 ± 1.48% in the perichondrium-preserved (n = 14) and -removed groups (n = 12, p < 0.05). The tissue ratios were cartilage: 38.18 ± 8.76 versus 52.97 ± 9.30%; lamellar bone: 18.49 ± 5.31 versus 0.82 ± 0.43%; bone marrow: 20.72 ± 6.27 versus 0.00 ± 0.00%; fibrous tissue: 10.13 ± 2.74 versus 5.81 ± 2.20%, and adipose tissue: 12.01 ± 4.48 versus 40.70 ± 7.83% in the perichondrium-preserved and -removed groups. The differences were all statistically significant (p < 0.05). Conclusion: A space-filling mass effect with minimal resorption of the cartilage pieces was observed in the perichondrium-removed group. In addition to this mass effect, the progenitor cells in the preserved perichondrium allowed active bone tissue regeneration and cartilage resorption in the perichondrium-preserved group.

show abstract

Toward the Identification of Mesenchymal Stem Cells in Bone Marrow and Peripheral Blood for Bone Regeneration

Cited by 39 publications

References 40 publications

Perioperative tumor cell dissemination in patients with primary or metastatic colorectal cancer

Perioperative tumor cell dissemination in patients with primary or metastatic colorectal cancer

When monocytes and platelets compete: The effect of platelet count on the flow cytometric measurement of monocyte CD36

Long-Term Histologic Changes and Effects of Perichondrium Preservation of Auricular Cartilage Grafted in Rabbit Tympanic Bullae

Contact Info

Product

Resources

About