Toward the development of Ac/Ds transposon-mediated gene tagging system for functional genomics in oat (Avena sativa L.)

Mahmoud, Mohannad; Zhou, Zhou; Kaur, Ravneet; Bekele, Wubishet A.; Tinker, Nicholas A.; Singh, Jaswinder

doi:10.1007/s10142-022-00861-9

Cited by 4 publications

(3 citation statements)

References 69 publications

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“…Compared to other cereals, oat has lagged behind in the genetic and genomic studies attributed to its large and complex genomes, estimated in the range of 4.12 Gb to 12.6 Gb. [90,91].…”

Section: Genotyping Tools In Oatsmentioning

confidence: 99%

“…In 2022, Mahmoud et al [90] published the development of an Ac/Ds transposonbased gene tagging system that could facilitate and expedite functional genomic studies in oat, leading to the discovery of genes at Ds insertion sites that showed homology to gibberellin 20-oxidase 3, (1,3;1,4)-beta-D-glucan synthase, and aspartate kinase.…”

Section: Perspectives On Biotechnological Tools To Empower Oats Again...mentioning

confidence: 99%

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Looking for Fusarium Resistance in Oats: An Update

Morcia,

Terzi,

Ghizzoni

et al. 2024

Agronomy

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In recent years, an increase of interest has arisen in oats due to their unique health-related properties. Fusarium Head Blight (FHB) is recognized as a major threat to oat production and safety. The Joint FAO/WHO Expert Committee on Food Additives (JECFA) assesses the risks of the presence of Fusarium-produced mycotoxins in foods and the tolerable intake level. This paper summarizes updates on Fusarium resistance in oats, describing the advances in phenotyping strategies and diagnostics methods and discussing the role of the infection process of the microbiome and bioactive compounds peculiar to oats. A special emphasis has been placed on the presentation of new genetic, genomic, and biotechnological knowledge and tools available today and their perspectives on breeding programs aiming to develop FHB-resistant genotypes.

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“…Compared to other cereals, oat has lagged behind in the genetic and genomic studies attributed to its large and complex genomes, estimated in the range of 4.12 Gb to 12.6 Gb. [90,91].…”

Section: Genotyping Tools In Oatsmentioning

confidence: 99%

Section: Perspectives On Biotechnological Tools To Empower Oats Again...mentioning

confidence: 99%

Looking for Fusarium Resistance in Oats: An Update

Morcia,

Terzi,

Ghizzoni

et al. 2024

Agronomy

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“…Oat (Avena sativa L.) is a cereal crop from the family Poaceae, widely known for its healthy and nutritious properties. The seeds of modern oat cultivars are rich in minerals, including Zn, Cu, Ca, Fe, and Mg [43]. Moreover, oat seeds have a high content of proteins [44], antioxidants [45], vitamins E and B [46], and β-glucan.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Metallothionein Gene Family in Avena sativa L. and the Gene Expression during Seed Germination and Heavy Metal Stress

Konieczna,

Mierek-Adamska,

Chojnacka

et al. 2023

Antioxidants

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Metallothioneins (MTs) are a family of small proteins rich in cysteine residues. The sulfhydryl group of metallothioneins can bind to metal ions, maintaining metal homeostasis and protecting the cells from damage caused by toxic heavy metals. Moreover, MTs can function as reactive oxygen species scavengers since cysteine thiols undergo reversible and irreversible oxidation. Here, we identified 21 metallothionein genes (AsMTs) in the oat (Avena sativa L.) genome, which were divided into four types depending on the amino acid sequences of putative proteins encoded by identified genes. Analysis of promoter sequences showed that MTs might respond to a variety of stimuli, including biotic and abiotic stresses and phytohormones. The results of qRT-PCR showed that all four types of AsMTs are differentially expressed during the first 48 hours of seed germination. Moreover, stress induced by the application of zinc, cadmium, and a mixture of zinc and cadmium affects the expression of oat MTs variously depending on the MT type, indicating that AsMT1-4 fulfil different roles in plant cells.

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