Toward the Design of Smart Delivery Systems Controlled by Integrated Enzyme-Based Biocomputing Ensembles

Abstract

We report herein the design of a smart delivery system in which cargo delivery from capped mesoporous silica (MS) nanoparticles is controlled by an integrated enzyme-based "control unit". The system consists of Janus-type nanoparticles having opposing Au and MS faces, functionalized with a pH-responsive β-cyclodextrin-based supramolecular nanovalve on the MS surface and two effectors, glucose oxidase and esterase, immobilized on the Au face. The nanodevice behaves as an enzymatic logical OR operator which is selectively fueled by the presence of D-glucose and ethyl butyrate.

A temperature-controlled method to produce Janus nanoparticles using high internal interface systems: Experimental and theoretical approaches

“…[22][23][24]. The main strategy of the process is completely similar to that discussed previously based on adsorbing hydrophilic particles onto oil/water interface.…”

“…The main strategy of the process is completely similar to that discussed previously based on adsorbing hydrophilic particles onto oil/water interface. The exposed surface of the adsorbed particles are then modified with a particular chemical reagent, commonly (3-mercaptopropyl)trimethoxysilane, which makes it possible to attach gold nanoparticles to one side of mesoporous silica nanoparticles [22][23][24].…”

Mesoporous silica materials for controlled delivery based on enzymes

“…29 The neoglycoenzyme was linked to the surface by the formation of cyclic boronic acid esters with (i) the naturallyoccurring glycosylation chains of the esterase and (ii) the synthetically-introduced lactose residues in the enzyme. The delivery system exhibited a two-step controlled cargo release triggered by glucose and ethyl butyrate.…”

“…30 The support consisted on Janus-type nanoparticles with gold and mesoporous silica faces on opposite sides. The mesoporous silica face allowed for cargo loading and functionalization whereas the Au face allowed the immobilization of certain enzymes.…”

Molecular gated nanoporous anodic alumina for the detection of cocaine

“…For the latter, the approach involves loading the porous support with a reporter and using a capping mechanism in such a way that cargo delivery is triggered by the presence of the target analyte. According to this principle, gated systems able to respond to anions910, cations111213, neutral molecules14151617 or biomolecules181920 have been recently developed. Most of these sensing materials are based on the use of mesoporous silica nanoparticles thanks to their well-known properties, such as large load capacity, biocompatibility and functionalization ease.…”