Toward the Authentication of Varietal Wines by the Analysis of Grape (<i>Vitis vinifera</i>L.) Residual DNA in Must and Wine Using Microsatellite Markers

Siret, René; Boursiquot, Jean‐Michel; Merle, M; Cabanis, J. C.; This, Patrice

doi:10.1021/jf991168a

Cited by 73 publications

(75 citation statements)

References 28 publications

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“…In this study, profiles generated by all microsatellite loci matched between samples from the same genotypes, reinforcing the view that nuclear SSR markers are suitable for certification purposes, as suggested by Baleiras-Couto and Eiras-Dias (2006), Savazzini and Martinelli (2006), and Siret et al (2000Siret et al ( , 2002.…”

Section: Nuclear Ssrsupporting

confidence: 73%

“…However, efficient DNA extraction and amplification from other matrices such as grape must and wine remain difficult, mainly due to: (a) plant DNA decomposition during the maceration process; (b) presence of microorganisms' DNA, namely yeasts; (c) DNA polymerase inhibitors such as polysaccharides, tannins, and polyphenols, present in matrices especially further down the processing chain. Nevertheless, several reports have been successful using must and experimental wine samples (Baleiras-Couto and Eiras-Dias 2006;Faria et al 2000;Garcia-Beneytez et al 2002;Nakamura et al 2007;Rodríguez-Plaza et al 2006;Siret et al 2000Siret et al , 2002. Grapevine collections were formerly described using ampelographic descriptions including morphological and phenological aspects (Alleweldt and Dettweiler 1986;Dettweiler 1993;Ortiz et al 2004;Santiago et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Markers for Assessing Must Varietal Origin

et al. 2012

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Wine quality and market value greatly depend on the grapevine varietal composition, which may be characteristic of specific regions. In order to defend the distinct regions, Denominations of Origin were defined to protect against fraudulent practices. In this study, we evaluated the efficiency of two microsatellite-based systems (microsatellite (SSR) and intermicrosatellite (ISSR)) for must varietal composition determination and their potential role in certification purposes. Eleven Vitis vinifera L. varieties from leaf and monovarietal must DNA samples were screened with six SSR and 14 ISSR primers to discriminate polymorphisms. Principal coordinates analysis was performed with DCENTER on the resultant data using unweighted pair group mathematical average and revealed that ISSRs markers were not suitable for certification procedures, whereas nuclear SSR markers presented a complete correspondence between leaf and must samples, demonstrating that they were adequate for traceability purposes.

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Section: Nuclear Ssrsupporting

confidence: 73%

Section: Introductionmentioning

confidence: 99%

Molecular Markers for Assessing Must Varietal Origin

et al. 2012

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“…Although ion-exchange type kits provided sufficient amounts of purified DNAs from samples of canned papaya fruits, it was necessary to select suitable primer combinations to amplify the transgenes. Attempts were made to apply DNA profiling methods to identify grapevine cultivars in must and wine using SSR markers (Faria et al 2000, Siret et al 2000, Garcia-Beneytez et al 2002. Cultivar identification was partially successful with genomic DNAs recovered from grape must and wine.…”

Section: Introductionmentioning

confidence: 99%

DNA Profiling of Fresh and Processed Fruits in Pear

et al. 2006

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A DNA profiling method was developed for fresh and processed fruits in pear. Four different DNA extraction methods were examined to isolate genomic DNAs from fresh pear fruits, i.e., CTAB-based method and methods based on 3 kinds of DNA extraction kits (DNeasy Plant Mini Kit, G2 buffer & Genomic-tip20/G, Nucleon Phytopure Plant DNA Extraction Kit). All the methods enabled to recover genomic DNAs from samples of fresh fruits with such a high quality that cultivar identification could be performed based on SSR markers. Among them, the G2 buffer & Genomic-tip20/G method gave the best results for samples of fresh as well as processed fruits. Partially degraded genomic DNAs that were isolated from samples of dried fruits could be amplified by all the tested SSR markers. SSR analysis revealed that genotypes from dried fruits were identical with those of the European pear cultivar 'Bartlett', indicating that cultivar identification could be successfully performed. Severely degraded genomic DNAs less than 500 bp in size were recovered from samples of canned fruits and fruit juice. The amount and quality of the extracted DNAs were sufficient to enable amplification by primers corresponding to high copy rDNA sequences, whereas no bands were produced by primers of chloroplast DNA sequences. Out of 15 SSR loci, 9 SSRs with target sequences less than 150-160 bp could successfully amplify fragments for genomic DNAs from samples of canned fruits and fruit juice. In contrast, no amplified fragments were observed for the remaining 6 SSRs with longer target sequences. DNA profiling and cultivar identification were successfully performed by using SSR markers to amplify short target sequences less than 150 bp.

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“…Primarily, the quality of wine falls on its vinification process and the grape species. Compounds which are considered as signature, like specific aromatic compounds, monoterpenes, trace compounds are utilized to test the grape varieties [29]. The quality of wine marks …”

Section: Dna Based Detection Of Adulteration and Gmo In Food Productsmentioning

confidence: 99%

“…But the results demonstrate that, extraction is more efficient in solid fraction then aqueous part. This may be due to the presence of DNA inhibitors in the aqueous fraction [29]. Late, same microsatellite loci and similar method was used as an attempt to analyze a grape variety Vitis vinifera in commercial must mixtures and also in experimental mixtures.…”

Section: Res Dev Materials Sci Research and Development In Materials Sciencementioning

confidence: 99%

DNA as a Biomaterial in Diagnosis of Food Adulteration and Food Safety Assurance

Muthu¹

2017

RDMS

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Apart from general applications of DNA, it can also be used as tool to assure the food safety, which is the main aspect of this review. Increasing GMO productions also increased the concern regarding the adulteration of GMO food products in our daily life. Countries around the world, raising the regulations regarding the GMO products to the consumer's table. Subsequently, the mode of adulteration in food products with GMO products feels difficult to identify. The food products also adulterer with low cost products which can also be detected by the isolation and identification of DNA from that product. The main focus on this review is to discuss the utilization of DNA as an effective tool to detect the adulteration and GMO content in various food products like oil, honey, beverages, baked products, animal feed, fried products, adulteration of milk and milk products. In the field of food safety, microbial contamination is also a major problem. Isolation of DNA from food can also reveals the presence of microbial contamination in the earlier stage. Also, the review emphases the way of utilizing DNA in differentiating organic foods from non-organic foods. ISSN: 2576-8840How to cite this article: Duraimurugan K, Narendhran S, Manikandan M. DNA as a Biomaterial A food should be free from microbial contamination until it reaches the consumers table. All kind of safety measures must be ensured by industries to make it possible. But still microbes are found in food products by any source, leading to the spoilage of food product and causing food borne infections to the consumers. Home cooked food samples collected from the villages in Malawi has shown to be contaminated upto 35% of pathogenic microbes and by further investigation, it was found that contamination of food samples by poor handling and unhygienic conditions [11]. Microbial contamination is a crucial problem in various food industries causing major economic losses. Various industries like dairy, poultry industries follow various cutting-edge technologies to reduce the microbial contamination in end product [12,13]. Microbial contamination can be explored by various physical methods including microscopic and culture depend approaches. But these techniques have various limitations in the confirmation of microbial presence. In present decades, PCR based methods are highly adopted due to its high accuracy and reliability [14].In the present review, we discuss the utilization of DNA as an effective tool in ensuring the quality and safety in various foods and processed food products (Figure 1). DiscussionDifferent DNA isolation methods from food products [20]. Apart from the standard protocols and commercial kits, various modified protocols are also adopted by researchers in order to enhance the efficiency of quality and quantity of extracted DNA [21]. DNA based detection of adulteration and GMO in food productsMilk and milk products: Milk stands as a source of high protein based food for vegetarians around the world. Commercial milk is available in the f...

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Toward the Authentication of Varietal Wines by the Analysis of Grape (Vitis viniferaL.) Residual DNA in Must and Wine Using Microsatellite Markers

Cited by 73 publications

References 28 publications

Molecular Markers for Assessing Must Varietal Origin

Molecular Markers for Assessing Must Varietal Origin

DNA Profiling of Fresh and Processed Fruits in Pear

DNA as a Biomaterial in Diagnosis of Food Adulteration and Food Safety Assurance

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