1994
DOI: 10.1073/pnas.91.20.9412
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Toward solving the folding pathway of barnase: the complete backbone 13C, 15N, and 1H NMR assignments of its pH-denatured state.

Abstract: The structures of the major folding intermediate, the transition state for folding, and the folded state of barnase have been previously characterized. We now add a further step toward a complete picture ofthe folding of barnase by reporting the backbone 15N, 13C, and 'H NMR assignments for barnase unfolded at pH 1.8 resonances and has very recently been applied to the assignment and structural characterization of the urea-and guanidinium chloride-denatured states of the FK506-binding protein (8).Barnase has… Show more

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Cited by 77 publications
(68 citation statements)
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“…Fig. 4 shows a comparison of the chemical shift dispersion of different nuclei, averaged over three unfolded proteins, apomyoglobin [19], barnase [5] and staphylococcal nuclease [6,9]. It is immediately obvious that the chemical shift dispersions for the backbone amide proton and backbone 15 N are large.…”
Section: Evaluation Of Chemical Shift Dispersionmentioning
confidence: 99%
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“…Fig. 4 shows a comparison of the chemical shift dispersion of different nuclei, averaged over three unfolded proteins, apomyoglobin [19], barnase [5] and staphylococcal nuclease [6,9]. It is immediately obvious that the chemical shift dispersions for the backbone amide proton and backbone 15 N are large.…”
Section: Evaluation Of Chemical Shift Dispersionmentioning
confidence: 99%
“…Chemical shift dispersion of various nucleus types averaged over all residue types for folded and unfolded proteins. The assigned chemical shifts are averaged for unfolded proteins apomyoglobin [19], barnase [5] and staphylococcal nuclease [6,9]. The assigned chemical shifts are averaged for five folded proteins, staphylococcal nuclease [10], human carbonic anhydrase [11], mutant dihydrofolate reducíase [12], recoverin [13] and ribonuclease A [14] The chemical shift dispersion is calculated as described in the text.…”
Section: Evaluation Of Chemical Shift Dispersionmentioning
confidence: 99%
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“…It is only recently that structural information for the denatured state has become available at atomic resolution because of developments in high field NMR spectroscopy (4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). These studies have focused on areas of persistent structure, with the aim of obtaining insight into the relationship between sequence, structure, and in particular, the mechanism of folding.…”
Section: Introductionmentioning
confidence: 99%