Toward Silencing the Burden of Malaria: Progress and Prospects for RNAi-based Approaches

Brown, Aaron; Catteruccia, Flaminia

doi:10.2144/000112117

Cited by 19 publications

(15 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…RNA interference (RNAi) is a highly favoured method for determining the function of gene products in mosquitoes (Brown and Catteruccia, 2006), because injection of gene-specific dsRNA reduces expression of the gene and creates a 'temporary null mutation'. This approach was used to silence expression of a putative A. gambiae AKHR, previously identified as a homolog of the D. melanogaster AKHR that is generously expressed throughout the body of all A. gambiae life stages .…”

Section: Rna Interferencementioning

confidence: 99%

Regulation of carbohydrate metabolism and flight performance by a hypertrehalosaemic hormone in the mosquito Anopheles gambiae

Kaufmann

Brown

2008

Journal of Insect Physiology

View full text Add to dashboard Cite

The role of adipokinetic hormones (AKHs) in the regulation of carbohydrate and lipid metabolism and flight performance was evaluated for females of the African malaria mosquito, Anopheles gambiae. Injection of various dosages of synthetic Anoga-AKH-I increased carbohydrate levels in the haemolymph and reduced glycogen reserves in sugar-fed females but did not affect lipid levels. Anoga-AKH-I enhanced the flight performance of both intact and decapitated sugar-fed females, during a 4 hour flight period. Anoga-AKH-II had no effect on carbohydrate or lipid levels or flight performance, thus its function remains unknown. Targeted RNA-interference lowered Anoga-AKH receptor expression in sugar-fed females, consequently injections of Anoga-AKH-I failed to mobilize glycogen reserves. Taken together, these results show that a primary role for the neurohormone, Anoga-AKH-I, is to elevate trehalose levels in the haemolymph of female mosquitoes.

show abstract

Section: Rna Interferencementioning

confidence: 99%

Regulation of carbohydrate metabolism and flight performance by a hypertrehalosaemic hormone in the mosquito Anopheles gambiae

Kaufmann

Brown

2008

Journal of Insect Physiology

View full text Add to dashboard Cite

show abstract

“…Recent developments, including transgenic vectors (Christophides 2005;Sperança and Capurro 2007), RNA interference (RNAi; Blair et al 2006;Brown and Catteruccia 2006;de la Fuente et al 2006a), and pathogen-derived antigens for development of transmission-blocking vaccines Kedzierski et al 2006;Saul 2007), have shown to be a promising alternative for the control of ticks and tick-borne pathogens. However, a limited number of vaccines against arthropods resulting in generation of antigen-specific antibodies and injury to the arthropod during feeding have been reported (de la Fuente et al 1998(de la Fuente et al , 2007aValenzuela et al 2001;Lal et al 2001;Almeida and Billingsley 2002;Suneja et al 2003;de la Fuente and Kocan 2003;Willadsen 2004;Milleron et al 2004;Titus et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin

et al. 2009

View full text Add to dashboard Cite

The control of arthropod vectors of pathogens that affect human and animal health is important for the eradication of vector-borne diseases. The ortholog of the tick-protective antigen, subolesin, was identified in Aedes albopictus and found to have conserved epitopes in ticks and mosquitoes. RNA interference with the tick and mosquito double-stranded RNA in three tick species resulted in significant gene knockdown and decreased tick weight and/or survival. Feeding Anopheles atroparvus, Aedes caspius, and Culex pipiens female mosquitoes on an A. albopictus subolesin hyperimmune serum resulted in 11 +/- 5% to 29 +/- 6% survival inhibition when compared to controls fed on preimmune serum. Feeding sand flies, Phlebotomus perniciosus, on antimosquito subolesin ortholog protein antibodies inhibited female survival and the number of larvae and adults obtained after hatching by 28 +/- 22% and 16 +/- 3%, respectively, when compared to controls. Vaccination with tick and mosquito subolesin ortholog proteins significantly reduced Ixodes scapularis tick infestation and weight in a similar way. However, vaccination with the recombinant mosquito subolesin ortholog antigen did not protect against Amblyomma americanum and Rhipicephalus sanguineus tick infestations. Collectively, these preliminary results provided the first evidence that development of vaccines may be possible for control of multiple arthropod vectors using subolesin orthologs but suggested that multiple antigens may be required to produce an effective vaccine.

show abstract

“…The application of RNAi strategies in Plasmodia is a highly contentious issue since there is currently no evidence for significant homologs of the RNAi machinery in this organism (Brown and Catteruccia, 2006). The use of chemical inhibition and functional genomics-based techniques therefore becomes more valuable but conclusive proof-of-principle of smallmolecule based target validation for P. falciparum is not as evident as for other organisms ( [Cowman and Crabb, 2003] and [Joachimiak et al, 2001]).…”

Section: Validation Of Drug Targetsmentioning

confidence: 99%

Exploring functional genomics for drug target and therapeutics discovery in Plasmodia

et al. 2008

View full text Add to dashboard Cite

Functional genomics approaches are indispensable tools in the drug discovery arena and have recently attained increased attention in antibacterial drug discovery research. However, the application of functional genomics to post-genomics research of Plasmodia is still in comparatively early stages. Nonetheless, with this genus having the most species sequenced of any eukaryotic organism so far, the Plasmodia could provide unique opportunities for the study of intracellular eukaryotic pathogens. This review presents the status quo of functional genomics of the malaria parasite including descriptions of the transcriptome, proteome and interactome. We provide examples for the in silico mining of the X-ome data sets and illustrate how X-omic data from drug challenged parasites might be used in elucidating amongst others, the mode-of-action of inhibitory compounds, validate potential targets and discover novel targets/therapeutics.Abbreviations: IDC, intraerythrocytic developmental cycle; MOA, mode-of-action; MudPIT, multidimensional protein identification technology; GO, gene ontology; SAGE, serial analysis of gene expression; ICAT, isotopically coded affinity tags; iTRAQ, isotope tags for relative and absolute quantification Article Outline

show abstract

Toward Silencing the Burden of Malaria: Progress and Prospects for RNAi-based Approaches

Cited by 19 publications

References 54 publications

Regulation of carbohydrate metabolism and flight performance by a hypertrehalosaemic hormone in the mosquito Anopheles gambiae

Regulation of carbohydrate metabolism and flight performance by a hypertrehalosaemic hormone in the mosquito Anopheles gambiae

Conservation and immunogenicity of the mosquito ortholog of the tick-protective antigen, subolesin

Exploring functional genomics for drug target and therapeutics discovery in Plasmodia

Contact Info

Product

Resources

About