Toward proteome‐wide exploration of proteins in dried blood spots using liquid chromatography‐coupled mass spectrometry

Nakajima, D.; Ohara, Osamu; Kawashima, Yusuke

doi:10.1002/pmic.202100019

Cited by 11 publications

(20 citation statements)

References 52 publications

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“…Not only hemoglobin was determined in this way, but also targeted determination of other proteins like human chorionic gonadotropin [32,33], insulin [34,35] and apolipoprotein A-I, apolipoprotein B [36] as well as multiplex strategies using multiple reaction monitoring (MRM) were reported [37][38][39][40]. Compared to these targeted analyses, shotgun proteomic analyses of DBS are still in their infancy [41]. As of now, only a few reports have been published on proteomic profiling from DBS [42,20,43,44].…”

Section: Introductionmentioning

confidence: 99%

Smart proteolysis samplers for pre‐lab bottom‐up protein analysis – Performance of on‐paper digestion compared to conventional digestion

Nguyen

Halvorsen

Thiede

et al. 2022

Separation Science Plus

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Here the relation between digestion of proteins by trypsin covalently bound to paper and trypsin in‐solution is investigated. The trypsin acting on paper is covalently bound. A trypsin concentration of 0.5% (w/v) results in the highest digestion activity of all concentrations tested. Additionally, it can be seen that trypsin on‐paper has retained approx. 50% of its activity. Unlike trypsin in‐solution, the stability of the smart proteolysis samplers was regarded to be stable for at least four months when kept refrigerated. Autolysis was very small for covalently bound trypsin: less than 2% compared to in‐solution trypsin. Proteomic analysis of diluted human serum showed more protein identifications (214) in‐solution digestions than on‐paper digestions (76). Also, higher coverage for the in‐solution digestion was obtained. Those proteins identified after on‐paper digestion with no or few disulfide bonds seem to have more similar sequence coverages compared to those identified after in‐solution digestion. Smart samplers allow the determination of at least 70–75 proteins without performing the overnight digestion. All in all, trypsin covalently bound to paper shows to retain high proteolytic activity and is a stable alternative for conventional digestions. In this way, smart proteolytic samplers show their feasibility in pre‐lab sample preparation.

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Section: Introductionmentioning

confidence: 99%

Smart proteolysis samplers for pre‐lab bottom‐up protein analysis – Performance of on‐paper digestion compared to conventional digestion

Nguyen

Halvorsen

Thiede

et al. 2022

Separation Science Plus

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“…This method is also considered promising for clinical diagnostics and for precision medicine, since it has been reported as an effective method for pre-analytic stages of diagnostics [2,3]. Research on DBS for application in medicine is growing and is being explored in combination with mass spectrometry (MS) [1] and in the context of different omics, like metabolomics [4,5], proteomics [6], and more recently lipidomics. While DBS is already well established in metabolomic analysis, its use in proteomics seems to face some challenges regarding protein analysis and degradation.…”

Section: Introductionmentioning

confidence: 99%

Dried blood spots in clinical lipidomics: optimization and recent findings

et al. 2022

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Dried blood spots (DBS) are being considered as an alternative sampling method of blood collection that can be used in combination with lipidomic and other omic analysis. DBS are successfully used in the clinical context to collect samples for newborn screening for the measurement of specific fatty acid derivatives, such as acylcarnitines, and lipids from whole blood for diagnostic purposes. However, DBS are scarcely used for lipidomic analysis and investigations. Lipidomic studies using DBS are starting to emerge as a powerful method for sampling and storage in clinical lipidomic analysis, but the major research work is being done in the pre-and analytical steps and procedures, and few in clinical applications. This review presents a description of the impact factors and variables that can affect DBS lipidomic analysis, such as the type of DBS card, haematocrit, homogeneity of the blood drop, matrix/chromatographic effects, and the chemical and physical properties of the analyte. Additionally, a brief overview of lipidomic studies using DBS to unveil their application in clinical scenarios is also presented, considering the studies of method development and validation and, to a less extent, for clinical diagnosis using clinical lipidomics. DBS combined with lipidomic approaches proved to be as effective as whole blood samples, achieving high levels of sensitivity and specificity during MS and MS/MS analysis, which could be a useful tool for biomarker identification. Lipidomic profiling using MS/MS platforms enables significant insights into physiological changes, which could be useful in precision medicine.

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“… 2 The use of dried biofluids is cheaper 1 3 4 and an array of studies show the samples are stable over a few days, which encompass the necessary postal time. 5 In addition, as a dried biofluid, the biological risk associated with the liquid form is significantly reduced. 2 6 Since the COVID-19 pandemic, home testing of nasal/throat swabs have become common practice.…”

Section: Introductionmentioning

confidence: 99%

Perceptions and acceptability of microsampling in children and young people: a single-centre survey

Chetwynd

Marro

Whitty

et al. 2022

bmjpo

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BackgroundThe use of at home microsampling devices, such as dried aliquots of blood and urine, for scientific research has expanded in recent years. These devices collect small volumes of biofluids which air dry and can be posted to central laboratories. In general, they are cheaper and more convenient, saving patients travel time and freeing up staff. In adult populations, this sampling method is well perceived, with >90% of samples being of adequate size and quality for scientific research. However, little literature on microsampling in paediatric populations exists. The aim of this study was to explore the perceptions and acceptability of microsampling in children and young people.MethodsOnline patient and public involvement was obtained by attending the Generation R Young Person’s Advisory Group Liverpool, to explore the perceptions of at home microsampling. During the session, the rationale and use of microsampling was demonstrated. The participants provided informal feedback during the interactive session and completed a short online questionnaire.ResultsA total of 13 children and young people attended the event and they were aged 10–17 years, of these 10 responded to an online questionnaire. The general feedback on microsampling was positive with 80% of respondents indicating they would be willing to participate in at home microsampling studies. Furthermore, 100% respondents reported being willing to provide both biofluids on a monthly basis and 40% would be willing to provide dried urine samples weekly.ConclusionsChildren and young people are supportive of at home microsampling for research purposes and this offers the possibility of widening participation to research.

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Toward proteome‐wide exploration of proteins in dried blood spots using liquid chromatography‐coupled mass spectrometry

Cited by 11 publications

References 52 publications

Smart proteolysis samplers for pre‐lab bottom‐up protein analysis – Performance of on‐paper digestion compared to conventional digestion

Smart proteolysis samplers for pre‐lab bottom‐up protein analysis – Performance of on‐paper digestion compared to conventional digestion

Dried blood spots in clinical lipidomics: optimization and recent findings

Perceptions and acceptability of microsampling in children and young people: a single-centre survey

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