Toward preparative resolution of chiral alcohols by an organic chemical method

Malic, Nino; Moorhoff, Cornelis M.; Sage, Valérie; Saylik, Dilek; Teoh, Euneace; Scott, Janet L.; Strauss, Christopher R.

doi:10.1039/b9nj00768g

Cited by 3 publications

(2 citation statements)

References 47 publications

(5 reference statements)

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“…Alcohols are crucial building blocks for the manufacturing of agrochemicals, flavor and fragrance compounds, and pharmaceutical drugs, especially chiral alcohols in their optically pure form. Typical methods to prepare them enantioselectively typically require the use of expensive, complicated chiral catalysts or transition metals, which can be toxic. − Biocatalytic approaches are considered an effective and green alternative due to their mild reaction conditions and remarkable enantioselectivity. A recent review by Chen and de Souza highlighted the recent developments in the field .…”

Section: Chromo- and Fluorogenic Probes For Alcoholsmentioning

confidence: 99%

Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

et al. 2023

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Many successful stories in enzyme engineering are based on the creation of randomized diversity in large mutant libraries, containing millions to billions of enzyme variants. Methods that enabled their evaluation with high throughput are dominated by spectroscopic techniques due to their high speed and sensitivity. A large proportion of studies relies on fluorogenic substrates that mimic the chemical properties of the target or coupled enzymatic assays with an optical read-out that assesses the desired catalytic efficiency indirectly. The most reliable hits, however, are achieved by screening for conversions of the starting material to the desired product. For this purpose, functional group assays offer a general approach to achieve a fast, optical read-out. They use the chemoselectivity, differences in electronic and steric properties of various functional groups, to reduce the number of false-positive results and the analytical noise stemming from enzymatic background activities. This review summarizes the developments and use of functional group probes for chemoselective derivatizations, with a clear focus on screening for enzymatic activity in protein engineering.

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Section: Chromo- and Fluorogenic Probes For Alcoholsmentioning

confidence: 99%

Enlightening the Path to Protein Engineering: Chemoselective Turn-On Probes for High-Throughput Screening of Enzymatic Activity

et al. 2023

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“…This has green chemistry principles as a prime driver, since this approach will deliver tuned selectivity, high yields, and recyclable versions Girard's reagents. 11 Herein, we describe the synthesis of a systematic library of ionic liquids which function as Girard's reagents (see Scheme 1). In order to achieve a versatile synthetic approach combined with relatively cheap and available reagents, the work described has focused on replacements for Girard's-T. Of course, it would be possible and straightforward to create a library of Girard's-P analogues (and indeed for potential reagents based on any other N-heterocycles), but here we have confined ourselves to demonstrating proof-of-principle, reagent versatility, and some unpredicted and fascinating properties of this class of reagent.…”

Section: Introductionmentioning

confidence: 99%