Toward point‐of‐care diagnostics: Running enzymatic assays on a photonic waveguide‐based sensor chip with a portable, benchtop measurement system

Abstract

We demonstrate a portable, compact system to perform absorption‐based enzymatic assays at a visible wavelength of 639 nm on a photonic waveguide‐based sensor chip, suitable for lab‐on‐a‐chip applications. The photonic design and fabrication of the sensor are described, and a detailed overview of the portable measurement system is presented. In this publication, we use an integrated photonic waveguide‐based absorbance sensor to run a full enzymatic assay. An assay to detect creatinine in plasma is simultaneously performed on both the photonic sensor on the portable setup and on a commercial microplate reader for a clinically relevant creatinine concentration range. We observed a high correlation between the measured waveguide propagation loss and the optical density measurement from the plate reader and measured a limit‐of‐detection of 4.5 μM creatinine in the sensor well, covering the relevant clinical range for creatinine detection.