Toward optimal-resolution NMR of intrinsically disordered proteins

Back to the Future: Nuclear Magnetic Resonance and Bioinformatics Studies on Intrinsically Disordered Proteins

“…High solvent exposure leads to similar local environments for all residues, leading to collapsed spectra with overlapping peaks. Additionally, the generally lower complexity of IDPs, relative to ordered proteins, leads to additional environmental symmetry between residues and further collapse of spectra (Felli and Pierattelli 2014;Nováček et al 2014).…”

“…Several approaches have been developed to overcome the high signal overlap of many IDPs (Nováček et al 2014). One of these approaches is sampling of spectra at a higher rate to resolve spectra overlap.…”

“…One of these approaches is sampling of spectra at a higher rate to resolve spectra overlap. However, optimal sampling is generally impractical due to long experiment times that would be required (Felli and Pierattelli 2014;Nováček et al 2014), and instead non-uniform sampling methods are employed to approximate the optimal sampling schedule (Mobli et al 2012). Lower complexity sequences are typically problematic because only neighboring residues are encoded in spectra, which leads to collapse due to degenerate sequence positions.…”

“…Lower complexity sequences are typically problematic because only neighboring residues are encoded in spectra, which leads to collapse due to degenerate sequence positions. One method to combat this collapse is to encode longer range sequence into the spectra (Nováček et al 2014), where the resulting high dimensional spectra are approximated with non-uniform sampling methods. Another method to com-bat collapsed spectra is the use of 13 C detection instead of 1 H detection (Bermel et al 2006).…”

Quarterly intrinsic disorder digest (April–May–June, 2014)

“…Konrat offers a general review on the contributions of NMR to the study of IDPs, 58 while Novacek et al provide a review in the on the progress toward optimal-resolution NMR. 59 Felli and Pierattelli discuss 13 C based methods in the study of IDPs. 60 In the Journal of Biomolecular NMR, Pantoja-Uceda and Santoro present new 13 C-detected experiments that can correlate consecutive 13 C'-15 N groups in IDPs and that also provide recognition of glycine residues, facilitating the assignment process.…”