Toward Fast and Quantitative Modification of Large Gold Nanoparticles by Thiolated DNA: Scaling of Nanoscale Forces, Kinetics, and the Need for Thiol Reduction

Zhang, Xu; Gouriye, Tony; Göeken, Kristian; Servos, Mark R.; Gill, Ron; Liu, Juewen

doi:10.1021/jp403946x

Cited by 58 publications

(83 citation statements)

References 42 publications

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“…Different methods have been evaluated for the preparation of the reporter probe conjugated with gold nanoparticles575859. Co-immobilization was explored as a means of controlling the probe surface density, as it is well known that spacing between the immobilised probes minimises steric hindrance, favouring accessibility to the complementary DNA strand.…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

Jauset‐Rubio

Svobodová

Mairal

et al. 2016

Sci Rep

140

113

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Sensitive, specific, rapid, inexpensive and easy-to-use nucleic acid tests for use at the point-of-need are critical for the emerging field of personalised medicine for which companion diagnostics are essential, as well as for application in low resource settings. Here we report on the development of a point-of-care nucleic acid lateral flow test for the direct detection of isothermally amplified DNA. The recombinase polymerase amplification method is modified slightly to use tailed primers, resulting in an amplicon with a duplex flanked by two single stranded DNA tails. This tailed amplicon facilitates detection via hybridisation to a surface immobilised oligonucleotide capture probe and a gold nanoparticle labelled reporter probe. A detection limit of 1 × 10−11 M (190 amol), equivalent to 8.67 × 105 copies of DNA was achieved, with the entire assay, both amplification and detection, being completed in less than 15 minutes at a constant temperature of 37 °C. The use of the tailed primers obviates the need for hapten labelling and consequent use of capture and reporter antibodies, whilst also avoiding the need for any post-amplification processing for the generation of single stranded DNA, thus presenting an assay that can facilely find application at the point of need.

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Section: Resultsmentioning

confidence: 99%

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

Jauset‐Rubio

Svobodová

Mairal

et al. 2016

Sci Rep

140

113

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“…40 nm silver and gold NPs (from TedPella Inc.) were functionalized with single stranded DNA oligonucleotides using the method by Liu et al 48 . The oligonucleotides HS-AAAAAAAAAACTCACGCTAC-GACTGACACC and HS-AAAAAAAAAAGACCTACTAAGACTACTACACAACCAGAGA-Biotin oligonucleotide were mixed in a 4:1 ratio.…”

Section: Methodsmentioning

confidence: 99%

Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers

Feizpour¹,

Stelter²,

Wong³

et al. 2017

ACS Sens.

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Viral membranes are nanomaterials whose fluidity depends on their composition, in particular the cholesterol (chol) content. As differences in the membrane composition of individual virus particles can lead to different intracellular fates, biophysical tools capable of sensing the membrane fluidity on the single-virus level are required. In this manuscript, we demonstrate that fluctuations in the polarization of light scattered off gold or silver nanoparticle (NP)-labeled virus-like-particles (VLPs) encode information about the membrane fluidity of individual VLPs. We developed plasmonic polarization fluctuation tracking microscopy (PFTM) which facilitated the investigation of the effect of chol content on the membrane fluidity and its dependence on temperature, for the first time on the single-VLP level. Chol extraction studies with different methyl-β-cyclodextrin (MβCD) concentrations yielded a gradual decrease in polarization fluctuations as function of time. The rate of chol extraction for individual VLPs showed a broad spread, presumably due to differences in the membrane composition for the individual VLPs, and this heterogeneity increased with decreasing MβCD concentration.

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“…40 nm gold NPs (from TedPella Inc.) were functionalized with single stranded DNA oligonucleotides using the method by Liu et al [34] The oligonucleotides HS -AAAAAAAAAACTCACGCTAC-GACTGACACC and HS -AAAAAAAAAAGACCTACTAAGACTACTACACAACCAGAGA- Biotin oligonucleotide were mixed in a 4:1 ratio. This mix was added to gold nanoparticle colloid in DDI water to yield a final concentration of 25 μM DNA and 1.5 nM nanoparticles in a total volume of 10 μl and incubated for 30 min at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Quantifying Lipid Contents in Enveloped Virus Particles with Plasmonic Nanoparticles

Feizpour

Akiyama

et al. 2014

Small

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Phosphatidylserine (PS) and monosialotetrahexosylganglioside (GM1) are examples of two host-derived lipids in the membrane of enveloped virus particles that are known to contribute to virus attachment, uptake, and ultimately dissemination. A quantitative characterization of their contribution to the functionality of the virus requires information about their relative concentrations in the viral membrane. Here, a gold nanoparticle (NP) binding assay for probing relative PS and GM1 lipid concentrations in the outer leaflet of different HIV-1 and Ebola virus-like particles (VLPs) using sample sizes of less than 3×106 particles is introduced. The assay evaluates both scattering intensity and resonance wavelength and determines relative NP densities through plasmon coupling as a measure for the target lipid concentrations in the NP-labeled VLP membrane. A correlation of the optical observables with absolute lipid contents is achieved by calibration of the plasmon coupling-based methodology with unilamellar liposomes of known PS or GM1 concentration. The performed studies reveal significant differences in the membrane of VLPs that assemble at different intracellular sites and pave the way to an optical quantification of lipid concentration in virus particles at physiological titers.

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Toward Fast and Quantitative Modification of Large Gold Nanoparticles by Thiolated DNA: Scaling of Nanoscale Forces, Kinetics, and the Need for Thiol Reduction

Cited by 58 publications

References 42 publications

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

Membrane Fluidity Sensing on the Single Virus Particle Level with Plasmonic Nanoparticle Transducers

Quantifying Lipid Contents in Enveloped Virus Particles with Plasmonic Nanoparticles

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