Toward Efficient Enzymes for the Generation of Universal Blood through Structure-Guided Directed Evolution

Kwan, David H.; Constantinescu, Iren; Chapanian, Rafi; Higgins, M.A.; Kötzler, Miriam P.; Samain, E.; Boraston, A.B.; Kizhakkedathu, Jayachandran N.; Withers, Stephen G.

doi:10.1021/ja5116088

Cited by 57 publications

(70 citation statements)

References 46 publications

(47 reference statements)

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“…[44] Because this enzyme cleaves several different substrates, as ingle coupled assay was employed that liberated af luorophore only when an exo-acting b-galactosidase further cleaved the target enzymesproduct ( Figure 3B). [44] Because this enzyme cleaves several different substrates, as ingle coupled assay was employed that liberated af luorophore only when an exo-acting b-galactosidase further cleaved the target enzymesproduct ( Figure 3B).…”

Section: Improving or Altering Existing Enzymesmentioning

confidence: 99%

“…[62] Evolution of improved CstII sialyl transferase activity was carried out using af luorescent bodipy-labelled derivative of lactose as an acceptor substrate. [44]), C) by change in FRET signal from one or more cleavage sites (Ref. Innovative strategies to screen for carbohydrate-active enzyme activity with generation of afluorescents ignal A) by direct hydrolysis, B) by coupled exo-glycosidase activity (Ref.…”

Section: Improving or Altering Existing Enzymesmentioning

confidence: 99%

See 1 more Smart Citation

High‐Throughput Approaches in Carbohydrate‐Active Enzymology: Glycosidase and Glycosyl Transferase Inhibitors, Evolution, and Discovery

Chao

Jongkees

2019

Angew Chem Int Ed

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Carbohydrates are attached and removed in living systems through the action of carbohydrate‐active enzymes such as glycosyl transferases and glycoside hydrolases. The molecules resulting from these enzymes have many important roles in organisms, such as cellular communication, structural support, and energy metabolism. In general, each carbohydrate transformation requires a separate catalyst, and so these enzyme families are extremely diverse. To make this diversity manageable, high‐throughput approaches look at many enzymes at once. Similarly, high‐throughput approaches can be a powerful way of finding inhibitors that can be used to tune the reactivity of these enzymes, either in an industrial, a laboratory, or a medicinal setting. In this review, we provide an overview of how these enzymes and inhibitors can be sought using techniques such as high‐throughput natural product and combinatorial library screening, phage and mRNA display of (glyco)peptides, fluorescence‐activated cell sorting, and metagenomics.

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Section: Improving or Altering Existing Enzymesmentioning

confidence: 99%

Section: Improving or Altering Existing Enzymesmentioning

confidence: 99%

High‐Throughput Approaches in Carbohydrate‐Active Enzymology: Glycosidase and Glycosyl Transferase Inhibitors, Evolution, and Discovery

Chao

Jongkees

2019

Angew Chem Int Ed

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“…This enables protein crystallographic studies of small crystals and crystals with large unit cells using single crystal X-ray diffraction, multiwavelength anomalous dispersion (MAD), and XAFS on crystals. For instance, in the area of health, with an ability to examine smaller protein crystals, a team from the University of British Columbia was able to gain insight into the development of a universal blood type by developing an enzyme that removes A-and B-type antigens from blood, which may improve the availability of life-saving procedures by limiting the need for matching blood types [11].…”

Section: Phase-i Experimental Facilitiesmentioning

confidence: 99%

The Brightest Light in Canada: The Canadian Light Source

Cutler

Chapman

Dallin

et al. 2017

QuBS

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Over forty years in the making, and one of Canada's largest scientific investments in those four decades, the Canadian Light Source (CLS), a third generation source of synchrotron light, was designed for high performance and flexibility and serves the diverse needs of the Canadian research community by providing brilliant light for applied and basic research programmes ranging from the far infrared to the hard X-ray regimes. Development of the scientific program at the CLS has been envisioned in four distinct phases. The first phase consists of the accelerator complex together with seven experimental facilities; the second phase adds six more experimental facilities and additional infrastructure to support them; the third phase adds seven more experimental facilities; and the fourth phase focuses on beamline and endstation upgrades, keeping the CLS as a state-of-the-art research centre. With the growth of a strong user community, the success of these experimental facilities will drive the future growth of the CLS.

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“…Yakın geçmişe kadar eritrosit yüzeyindeki A ve B antijenlerinin enzimatik etki ile elimine edilip O grubunda evrensel eritrosit konsantresi elde edilmesi yada A ve B antijenlerinin yanı sıra eritrosit yüzeyin-deki diğer antijenik yapılarında maskelenmesi ile antijenik özelliği taşımayan eritrosit konsantrelerinin transfüzyonu hedeflenmiş durumdaydı (3,4,(6)(7)(8)(9)(10)(11)(12) . Bir yandan bu yöndeki çalışmalar devam ederken yeni bir çözüm yolu olarak ayrı ayrı çalışmalarla olsa da birleştiğinde hedefi laboratuvar ortamında eritrosit konsantrelerinin üretimi olan çalışmalar da dikkati çekmektedir.…”

Section: A Evrensel Eritrosit Konsantreleriunclassified