Toward development of an implantable tissue engineered liver

Davis, Matthew W.; Vacanti, Joseph P.

doi:10.1016/0142-9612(96)85575-x

Cited by 113 publications

(47 citation statements)

References 47 publications

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“…Such scaffolds should function in a manner similar to the extracellular matrix (ECM) found in normal tissue when engineering substitutes for bone, cartilage, skin, liver and nerve [3][4][5][6][7][8][9]. Recently, the field has focused on developing scaffolds that induce stem cells to differentiate into desired phenotypes necessary for proper tissue formation and function [8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of poly (ϵ-caprolactone) microfiber scaffolds with varying topography and mechanical properties for stem cell-based tissue engineering applications

Mohtaram

Ahmed

et al. 2013

Journal of Biomaterials Science, Polymer Edition

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Abstract.Highly porous poly (ε-caprolactone) microfiber scaffolds can be fabricated using electrospinning for tissue engineering applications. Melt electrospinning produces such scaffolds by direct deposition of a polymer melt instead of dissolving the polymer in a solvent as performed during solution electrospinning. The objective of this study was to investigate the significant parameters associated with the melt electrospinning process that influence fiber diameter and scaffold morphology, including processing temperature, collection distance, applied voltage and nozzle size. The mechanical properties of these microfiber scaffolds varied with microfiber diameter. Additionally, the porosity of scaffolds was determined by combining experimental data with mathematical modeling. To test the cytocompatability of these fibrous scaffolds, we seeded neural progenitors derived from murine R1 embryonic stem cell lines onto these scaffolds where they could survive, migrate, and differentiate into neurons, demonstrating the potential of these melt electrospun scaffolds for tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Fabrication of poly (ϵ-caprolactone) microfiber scaffolds with varying topography and mechanical properties for stem cell-based tissue engineering applications

Mohtaram

Ahmed

et al. 2013

Journal of Biomaterials Science, Polymer Edition

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“…In the literature, hepatocyte survival was proven in the following loci: interscapular fat pads, intraperitoneal and subcutaneous tissue (both when attached to an extracellular matrix such as collagen-coated microcarrier beads), and pancreas [10,18,19] . When hepatocytes were transplanted into the pulmonary vascular bed or directly into the lung parenchyma, only limited survival of these cells occurred [20] .…”

Section: Discussionmentioning

confidence: 99%

Intrasplenic or Subperitoneal Hepatocyte Transplantation to Increase Survival after Surgically Induced Hepatic Failure?

et al. 2008

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Background: As a basis for future clinical questions, we evaluated the efficacy of hepatocyte transplantation in a surgical model using a subperitoneal or intrasplenic approach for cell implantation. Methods: In rats, acute liver failure was induced by subtotal hepatectomy. Series of allogenic hepatocyte transplantations were performed by varying cell number, site, and sequence of cell transplantation. Results: Following subperitoneal or intrasplenic cell implantation subsequent to liver surgery, no survival benefit was achieved when compared to the control groups. However, intrasplenic cell implantation 24 h prior to liver surgery revealed a statistically significantly higher animal survival (72 vs. 29%). Conclusion: According to our experience, both timing and site of cell implantation played an important role in hepatocyte transplantation. Intrasplenic hepatocyte transplantation 1 day before liver surgery showed the best results in terms of survival. Consequently, we were able to establish a model of hepatocyte transplantation which may be the basis for further investigations evaluating potential treatment modalities to overcome deleterious postoperative liver insufficiency.

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“…It should be noted that, in recent years, scaffolds are recognized as an alternative material with porous structure for advanced applications, such as bioactive molecule delivery, medical implants, cultured artifi-cial organs, and tissue engineering. [8][9][10][11][12] Until now, the techniques known for preparing scaffold are, for example, polymer assembly, phase separation, and electrospinning. The polymer assembly is a preformed structure based on molecular interactions, such as hydrophilic, hydrophobic, and ionic interactions, etc.…”

Section: Introductionmentioning

confidence: 99%

A novel soft and cotton‐like chitosan‐sugar nanoscaffold

2006

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A novel type of chitosan nanoscaffold with a soft and cotton-like appearance is proposed. The key to success is based on two points: (i) the change in morphology of chitin whisker to chitosan nanoscaffold and (ii) the surface modification of the nanoscaffold chitosan with a sugar unit. Simple deacetylation of chitin whisker gives a colloidal solution of chitosan, of which the chitosan is in a nanoscaled scaffold. Surface functionalization of the chitosan nanoscaffold with lactose or maltose via a heterogeneous system in water at room temperature results in a soft and cotton-like chitosan containing mesopores. As all steps are organic solvent free, this chitosan-sugar nanoscaffold might be a promising material for biopolymer-supported tissue engineering.

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Toward development of an implantable tissue engineered liver

Cited by 113 publications

References 47 publications

Fabrication of poly (ϵ-caprolactone) microfiber scaffolds with varying topography and mechanical properties for stem cell-based tissue engineering applications

Fabrication of poly (ϵ-caprolactone) microfiber scaffolds with varying topography and mechanical properties for stem cell-based tissue engineering applications

Intrasplenic or Subperitoneal Hepatocyte Transplantation to Increase Survival after Surgically Induced Hepatic Failure?

A novel soft and cotton‐like chitosan‐sugar nanoscaffold

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