Toward creating cell membrane glyco-landscapes with glycan lipid constructs

Korchagina, Elena; Tuzikov, Alexander; Formanovsky, A. A.; Popova, I. S.; Henry, Stephen; Bovin, Nicolai V.

doi:10.1016/j.carres.2012.03.044

Cited by 36 publications

(52 citation statements)

References 25 publications

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“…In this regard, single-tailed lipids have been used by us and others to couple molecules onto stem cell surfaces [25, 44]. Function-Spacer-Lipid (FSL) constructs utilizing a two-tailed lipid, DOPE, have also been used commercially to couple various groups to cell and virion surfaces [45, 46]. Upon performing a systematic comparison of the two approaches, our data show that PPG requires less time and results in more stable lipid incorporation compared to DPG.…”

Section: Discussionmentioning

confidence: 99%

Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model

Weil

Palka

et al. 2016

Biomaterials

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Promising results are emerging in clinical trials focused on stem cell therapy for cardiology applications. However, the low homing and engraftment of the injected cells to target tissue continues to be a problem. Cellular glycoengineering can address this limitation by enabling the targeting of stem cells to sites of vascular injury/inflammation. Two such glycoengineering methods are presented here: i. The non-covalent incorporation of a P-selectin glycoprotein ligand-1 (PSGL-1) mimetic 19Fc[FUT7+] via lipid-protein G fusion intermediates that intercalate onto the cell surface, and ii. Over-expression of the α(1,3)fucosyltransferse FUT7 in cells. Results demonstrate the efficient coupling of 19Fc[FUT7+] onto both cardiosphere-derived cells (CDCs) and mesenchymal stem cells (MSCs), with coupling being more efficient when using protein G fused to single-tailed palmitic acid rather than double-tailed DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine). This non-covalent cellular modification was mild since cell proliferation and stem-cell marker expression was unaltered. Whereas coupling using 19Fc[FUT7+] enhanced cell capture on recombinant P-selectin or CHO-P cell surfaces, α(1,3)fucosylation was necessary for robust binding to E-selectin and inflamed endothelial cells under shear. Pilot studies confirm the safety and homing efficacy of the modified stem cells to sites of ischemia-reperfusion in the porcine heart. Overall, glycoengineering with physiological selectin-ligands may enhance stem cell engraftment.

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Section: Discussionmentioning

confidence: 99%

Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model

Weil

Palka

et al. 2016

Biomaterials

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“…To avoid this issue, with larger func tional heads a two stage approach can be used, where the FSL functional head group is a non glycan reactive func tional group that is able to react secondarily with a suit ably modified glycan. Typically, this would involve the use of an FSL with biotin as the functional head, which could then be used to capture a biotinylated glycan via an avidin bridge [15]. However, a variety of alternative approaches can be used, for example, FSLs with reactive functional groups such as maleimide, click chemistry, enzyme-sub strate interactions, or chelators where appropriate sec ondarily labeled glycans are available.…”

Section: Synthetic Glycolipid Like Constructs (Fsls)mentioning

confidence: 99%

“…Full descriptions of the preparation of FSLs with different spacers are given elsewhere [15]. Here we briefly overview the general steps.…”

Section: Synthesis Of Glycolipid Like Constructs (Fsls)mentioning

confidence: 99%

“…To overcome the above mentioned limitations, we adopted an approach that was to create water dispersible glycolipid like constructs by attaching glycans to lipids via spacers that facilitated water dispersibility, to create so called function-spacer-lipid (FSL) constructs [15]. Furthermore, the design of FSLs was constrained by other criteria (to enable in vivo use) including that the constructs must be able to spontaneously and stably insert into cell membranes, and the spacer and lipid per se must be biologically inert [15].…”

mentioning

confidence: 99%

“…Furthermore, the design of FSLs was constrained by other criteria (to enable in vivo use) including that the constructs must be able to spontaneously and stably insert into cell membranes, and the spacer and lipid per se must be biologically inert [15]. Serendipitously, it was later found that these FSLs were also able to spontaneously self assemble in a few seconds on almost any non biolog ical surface including metals, plastics, and paper to form a glycan coating, which is stable (not washed off) in plas ma and buffer solutions [16].…”

mentioning

confidence: 99%

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Synthetic glycolipid-like constructs as tools for glycobiology research, diagnostics, and as potential therapeutics

Korchagina

Henry

2015

Biochemistry Moscow

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Function-spacer-lipid (FSL) constructs are amphiphilic molecules that are able to disperse in water and then self-assemble into cell membranes or onto solid surfaces. Modification of a biological or non-biological surface is very easy and achieved by simple contact of the surface with an appropriately buffered solution containing one or more FSLs. When the functional head group of the FSL is a glycan, glycan modified surfaces can be rapidly formed. Once cells, viruses, or solid surfaces are FSL modified with either simple or complex glycans, they can be used in vitro and/or in vivo to measure interactions with cells, viruses, antibodies, and lectins. FSLs have already been used in a variety of techniques including antibody specificity mapping, antibody/toxin neutralization, diagnostic assays, immune system manipulation, and animal modeling of transfusion reactions. FSLs offer the easiest and fastest method available to achieve a glycan-modified surface.

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Biofunctionalizing nanofibers with carbohydrate blood group antigens

Barr

Kannan²,

Korchagina

et al. 2016

Biopolymers

Self Cite

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A rapid and simple method of biofunctionalising nylon, cellulose acetate, and polyvinyl butyral electrospun nanofibers with blood group glycans was achieved by preparing function-spacer-lipid constructs and simply contacting them to fibers with a piezo inkjet printer. A series of water dispersible amphipathic glycan-spacer constructs were synthesized representing a range ABO and related blood group antigens. After immediate contact of the amphipathic glycan-spacer constructs with nanofiber surfaces they self-assembled and were detectable by enzyme immunoassays with high sensitivity and specificity.

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Toward creating cell membrane glyco-landscapes with glycan lipid constructs

Cited by 36 publications

References 25 publications

Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model

Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model

Synthetic glycolipid-like constructs as tools for glycobiology research, diagnostics, and as potential therapeutics

Biofunctionalizing nanofibers with carbohydrate blood group antigens

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