Toward building the cow folliculome

Sirard, Marc‐André

doi:10.1016/j.anireprosci.2014.06.025

Cited by 8 publications

(2 citation statements)

References 30 publications

(22 reference statements)

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“…In the developing follicle, acquisition of oocyte competence involves interplay between a multitude of intrinsic and extrinsic factors, which all act to bring about rapid development of distinct gene expression profiles in different follicular cells (Wigglesworth et al 2014. This is particularly apparent in granulosa cells (Sirard 2014), in which gene expression patterns are important not only for the ovulation and luteinization processes but also for the developmental competence of the oocyte contained therein (Assidi et al 2008, Hamel et al 2010. Interestingly, FSH has been implicated in acquisition of oocyte developmental competence, both in vivo and in vitro (Sirard et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Meta-analysis of gene expression profiles in granulosa cells during folliculogenesis

Khan¹,

Fournier²,

Dufort³

et al. 2016

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Folliculogenesis involves coordinated profound changes in different follicular compartments and significant modifications of their gene expression patterns, particularly in granulosa cells. Huge datasets have accumulated from the analyses of granulosa cell transcriptomic signatures in predefined physiological contexts using different technological platforms. However, no comprehensive overview of folliculogenesis is available. This would require integration of datasets from numerous individual studies. A prerequisite for such integration would be the use of comparable platforms and experimental conditions. The EmbryoGENE program was created to study bovine granulosa cell transcriptomics under different physiological conditions using the same platform. Based on the data thus generated so far, we present here an interactive web interface called GranulosaIMAGE (

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Section: Introductionmentioning

confidence: 99%

Meta-analysis of gene expression profiles in granulosa cells during folliculogenesis

Khan¹,

Fournier²,

Dufort³

et al. 2016

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“…However, despite known interactions between compartments, gene expression profiles have been studied in specific cell types, apparently to maximize technical coherence. Such an approach overlooks the possibility that some pathway active in the follicle as a whole ultimately determines oocyte competence (27). This underscores the need to perform integrative meta-analyses of different cell transcriptomes to gain a fuller understanding of follicle physiology.…”

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confidence: 99%

Transcriptome meta-analysis of three follicular compartments and its correlation with ovarian follicle maturity and oocyte developmental competence in cows

Khan

Landry

Fournier

et al. 2016

Physiological Genomics

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Sirard MA. Transcriptome meta-analysis of three follicular compartments and its correlation with ovarian follicle maturity and oocyte developmental competence in cows. Physiol Genomics 48: 633-643, 2016. First published July 8, 2016 doi:10.1152/physiolgenomics.00050.2016.-Oocyte developmental competence in superstimulated cows is dependent in part on the duration of the FSH coasting. FSH coasting refers to superstimulation with FSH (2 days of endogenous FSH following follicle ablation and 3 days of FSH injections) followed by no FSH for a specific duration. The optimal duration varies among individuals. FSH coasting appears to modulate the transcriptome of different follicular compartments, which cooperate as a single functional unit. However, the integrative effects of FSH coasting on different follicular compartments remain ambiguous. Meta-analysis of three independent transcriptome studies, each focused on a single cell type (granulosa, cumulus, and oocyte) during FSH coasting, allowed the identification of 12 gene clusters with similar time-course expression patterns in all three compartments. Network analysis identified HNF4A (involved in metabolic functions) and ELAVL1 (an RNA-binding protein) as hub genes regulated respectively upward and downward in the clusters enriched at the optimal coasting time, and APP (involved in mitochondrial functions) and COPS5 (a member of the COP9 signalosome) as hub genes regulated respectively upwards and downwards in the clusters enriched progressively throughout the coasting period. We confirmed the effects on HNF4A downstream targets (TTR, PPL) and other hub genes (ELAVL1, APP, MYC, and PGR) in 30 cows with RT-quantitative PCR. The correlation of hub gene expression levels with FSH coasting indicated that a combination of these genes could predict oocyte competence with 83% sensitivity, suggesting that they are potential biomarkers of follicle differentiation. These findings could be used to optimize FSH coasting on an individual basis. ovarian follicle; oocyte competence; transcriptomics; meta-analysis; FSH coasting ASSISTED REPRODUCTIVE TECHNOLOGIES have been used successfully in a wide range of research and clinical applications. However, the success rate remains low, despite increasing research efforts (19). Better understanding of ovarian physiology and of the acquisition of oocyte developmental competence in particular is needed to improve the efficiency of fertility treatments in the clinical setting and in livestock production.

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In Vitro Maturation of Human Oocytes: Current Practices and Future Promises

Combelles

2016

Human Reproduction

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Toward building the cow folliculome

Cited by 8 publications

References 30 publications

Meta-analysis of gene expression profiles in granulosa cells during folliculogenesis

Meta-analysis of gene expression profiles in granulosa cells during folliculogenesis

Transcriptome meta-analysis of three follicular compartments and its correlation with ovarian follicle maturity and oocyte developmental competence in cows

In Vitro Maturation of Human Oocytes: Current Practices and Future Promises

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