Toward assessment of resection margins using hyperspectral diffuse reflection imaging (400–1,700 nm) during tongue cancer surgery

Abstract

Background and ObjectivesThere is a clinical need to assess the resection margins of tongue cancer specimens, intraoperatively. In the currentex vivostudy, we evaluated the feasibility of hyperspectral diffuse reflectance imaging (HSI) for distinguishing tumor from the healthy tongue tissue.Study Design/Materials and MethodsFresh surgical specimens (n = 14) of squamous cell carcinoma of the tongue were scanned with two hyperspectral cameras that cover the visible and near‐infrared spectrum (400–1,700 nm). Each pixel of the hyperspectral image represents a measure of the diffuse optical reflectance. A neural network was used for tissue‐type prediction of the hyperspectral images of the visual and near‐infrared data sets separately as well as both data sets combined.ResultsHSI was able to distinguish tumor from muscle with a good accuracy. The diagnostic performance of both wavelength ranges (sensitivity/specificity of visual and near‐infrared were 84%/80% and 77%/77%, respectively) appears to be comparable and there is no additional benefit of combining the two wavelength ranges (sensitivity and specificity were 83%/76%).ConclusionsHSI has a strong potential for intra‐operative assessment of tumor resection margins of squamous cell carcinoma of the tongue. This may optimize surgery, as the entire resection surface can be scanned in a single run and the results can be readily available. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

Over 1000 nm Near-Infrared Multispectral Imaging System for Laparoscopic In Vivo Imaging

“…Spectral imaging with a high wavelength resolution is called hyperspectral imaging (HSI) [9,10]. Various resected cancers have been analyzed using HSI [11][12][13][14][15][16]. For instance, Sato et al reported that NIR-HSI could be used to identify tumors covered by normal mucosa [17].…”

Laparoscopic system for simultaneous high-resolution video and rapid hyperspectral imaging in the visible and near-infrared spectral range

“…Hyperspectral imaging (HSI) has been used in many fields of biomedical research and clinical applications in recent years. 1 Promising results were reported for the estimation of organ perfusion, 2,3 differentiation of anatomical structures, 4,5 wound assessment, 6,7 and the detection of cancerous tissue [8][9][10][11] in animal experiments and clinical studies. 12 However, the majority of the *Address all correspondence to Hannes Köhler, E-mail: Hannes.…”

Cutting-edge technology for oncological oral surgery

“…The extension of the spectral range toward the infrared spectrum, where absorption of light by blood is negligible, should make the technology more applicable for use during surgery. Results of two different studies reporting on 14 tongue specimens and 21 tongue, larynx, pharynx and mandible specimens using a VIS HSI camera were comparable in the discriminative power of tumor and healthy tissue (sensitivity of 84% and 81%; specificity of 77% and 80%, respectively) (46,47). Recently, Halicek et al reported on a larger study on 102 patients using a deep learning model to detect squamous cell carcinoma with VIS HSI in less than 2 minutes with a sampling depth of less than 3 mm (48).…”

“…The image acquired by hyperspectral imaging (HSI) is constructed of a diffuse reflectance spectrum for each pixel, allowing to evaluate the whole resection surface in one view. Results are reported for the detection of the reflected light in the visual (VIS) part of the wavelength spectrum (400-950 nm) and the near infrared (NIR) part (950-1700 nm) (46)(47)(48). The extension of the spectral range toward the infrared spectrum, where absorption of light by blood is negligible, should make the technology more applicable for use during surgery.…”

“…Recently, Halicek et al reported on a larger study on 102 patients using a deep learning model to detect squamous cell carcinoma with VIS HSI in less than 2 minutes with a sampling depth of less than 3 mm (48). a sensitivity of 80% and a specificity of 77% were obtained with the NIR camera on tongue specimens (46).…”