Toward a Reliable and Generic Applicable Soluble Microbial Polymer Extraction Protocol

which has been archived on the university repository Lirias (https://lirias.kuleuven.be/) of the Katholieke Universiteit Leuven.The content is identical to the content of the published paper, but without the final typesetting by the publisher.When referring to this work, please cite the full bibliographic info:Van Dierdonck, J., Van den Broeck, R., Vervoort, E., Van Impe, J., Smets, I. (2013). Effect of alternating influent carbon source composition on activated sludge bioflocculation. Journal of Biotechnology, 167 (3),[225][226][227][228][229][230][231][232][233][234] The journal and the original published paper can be found at: http://www.journals.elsevier.com/journal-of-biotechnology http://dx.doi.org/10.1016/j.jbiotec.2013.07.012 The corresponding author can be contacted for additional info.Conditions for open access are available at: http://www.sherpa.ac.uk/romeo/ Postprint version of paper published in Journal of Biotechnology 2013, vol. 167, issue 3, p. 225--234. The content is identical to the published paper, but without the final typesetting by the publisher. AbstractThe impact of alternating influent carbon sources, i.e., glucose and starch, on activated sludge bioflocculation was investigated. To this end, four lab-scale reactors were operated during a long-term experiment. During this period the influent carbon source ratio (glucose/starch) was alternated every 7 or 35 days (i.e., a fast and slow switching frequency). Bioflocculation was monitored throughout the entire experiment using an extensive set of parameters, including macroscopic and microscopic activated sludge characteristics. Sludge hydrophobicity remained high (> 80%) throughout the experiment indicating good bioflocculation. However, sludge settleability decreased for all four reactors after a 60 day adaptation period to the applied alternation in influent carbon source. During this adaptation period, floc size decreased due to the release of microcolonies. The subsequent period was characterized by a decrease in settleability, coinciding with a release of primary particles and an increase in floc size. The observed phenomena could be linked with the protein concentration near the floc surface. This fraction mainly consists of hydrolytic enzymes necessary for the degradation of starch and is responsible for a progressive deterioration of the EPS matrix. The results of this specific study indicate to be independent of the influent carbon source ratio or switching frequency.

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“…SMP are extracted and analysed according to Van Dierdonck et al (2012a) and Van Dierdonck et al (2012b), respectively.…”

Section: Extracellular Polymeric Substances Eps Can Be Divided In Twmentioning

confidence: 99%