Toward a phylogenetic reclassification of the subfamily Ambavioideae (Annonaceae): establishment of a new subfamily and a new tribe

Abstract

A molecular phylogeny of the subfamily Ambavioideae (Annonaceae) was reconstructed using up to eight plastid DNA regions (matK, ndhF, and rbcL exons; trnL intron; atpB-rbcL, psbA-trnH, trnL-trnF, and trnS-trnG intergenic spacers). The results indicate that the subfamily is not monophyletic, with the monotypic genus Meiocarpidium resolved as the second diverging lineage of Annonaceae after Anaxagorea (the only genus of Anaxagoreoideae) and as the sister group of a large clade consisting of the rest of Annonaceae. Consequently, a new subfamily, Meiocarpidioideae, is established to accommodate the enigmatic African genus Meiocarpidium. In addition, the subfamily Ambavioideae is redefined to contain two major clades formally recognized as two tribes. The tribe Tetramerantheae consisting of only Tetrameranthus is enlarged to include Ambavia, Cleistopholis, and Mezzettia; and Canangeae, a new tribe comprising Cananga, Cyathocalyx, Drepananthus, and Lettowianthus, are erected. The two tribes are principally distinguishable from each other by differences in monoploid chromosome number, branching architecture, and average pollen size (monads). New relationships were retrieved within Tetramerantheae, with Mezzettia as the sister group of a clade containing Ambavia and Cleistopholis.

Molecular phylogeny of Annonaceae species from Binh Chau-Phuoc Buu Nature Reserve based on two chloroplast gene regions

“…The result was consistent with many previous studies on the phylogeny analysis of Annonaceae in particular and in plants in general. In fact, the longer the DNA sequences used in the phylogeny analysis are, the more accurate the arrangement of species on the phylogenetic tree is (Doyle & Thomas, 1997;Rodriguez et al, 2016;Guo et al, 2017;Chaowasku, 2020).…”

DNA BARCODING OF CANANGA ODORATA (LAM. HOOK.F. & THOMSON) USING CHLOROPLAST GENES IN NORTH SUMATRA, INDONESIA

“…Cananga, Drepananthus, and Cyathocalyx also clustered on the parsimony tree based on the psbA-trnH spacer, trnL-F, rbcL, and matK sequences (Surveswaran et al, 2010). In addition, phylogenetic analysis of the Ambavioideae subfamily (Annonaceae) based on eight plastid DNA regions (matK, ndhF, and rbcL exons; trnL intron; atpB-rbcL, psbA-trnH, trnL-trnF, and trnS trnG intergenic spacers) grouped Cananthus, Cyathocalyx, and Lettowianthus into the same group based on the maximum likelihood method phylogenetic tree analysis (Chaowasku, 2020). Based on the analysis results, the markers rbcL, matK-trnK, and trnL-F have the potential as DNA barcodes in C. odorata plants native to North Sumatra, Indonesia.…”

Nuclear phylogenomics of angiosperms and insights into their relationships and evolution

“…A phylogenetic tree including 65 genera of Annonaceae was reconstructed using 317 enriched exonic regions, with infra‐family relationships generally consistent with plastid analysis (Couvreur et al, 2019; Xue et al, 2020). The genus Meiocarpidium was distant from two other sampled genera of Ambavioideae in the nuclear analysis and was separated as a new subfamily, Meiocarpidioideae, in a recent Annonaceae classification (Chaowasku, 2020). However, the relationship between Anaxagoreoideae and Ambavioideae was not resolved in the nuclear phylogenomic analyses due to the lack of an outgroup from non‐Annonaceae groups (Couvreur et al, 2019).…”