Toward a microbial palm oil substitute: oleaginous yeasts cultured on lignocellulose

Whiffin, Fraeya; Santomauro, Fabio; Chuck, Christopher J.

doi:10.1002/bbb.1641

Cited by 41 publications

(32 citation statements)

References 175 publications

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“…Finally, it is worth highlighting that fungi, yeast, and bacteria are emerging as microbial oil producers. Although this approach is still on its early stages their high growth rates, productivities and yields while using a wide variety of carbon source makes them a worth considering alternative to microalgae-base microbial lipids (Muniraj et al, 2015; Whiffin et al, 2016; Zhang et al, 2016). …”

Section: Resource Recovery For a Circular Economymentioning

confidence: 99%

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

et al. 2017

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Limits in resource availability are driving a change in current societal production systems, changing the focus from residues treatment, such as wastewater treatment, toward resource recovery. Biotechnological processes offer an economic and versatile way to concentrate and transform resources from waste/wastewater into valuable products, which is a prerequisite for the technological development of a cradle-to-cradle bio-based economy. This review identifies emerging technologies that enable resource recovery across the wastewater treatment cycle. As such, bioenergy in the form of biohydrogen (by photo and dark fermentation processes) and biogas (during anaerobic digestion processes) have been classic targets, whereby, direct transformation of lipidic biomass into biodiesel also gained attention. This concept is similar to previous biofuel concepts, but more sustainable, as third generation biofuels and other resources can be produced from waste biomass. The production of high value biopolymers (e.g., for bioplastics manufacturing) from organic acids, hydrogen, and methane is another option for carbon recovery. The recovery of carbon and nutrients can be achieved by organic fertilizer production, or single cell protein generation (depending on the source) which may be utilized as feed, feed additives, next generation fertilizers, or even as probiotics. Additionlly, chemical oxidation-reduction and bioelectrochemical systems can recover inorganics or synthesize organic products beyond the natural microbial metabolism. Anticipating the next generation of wastewater treatment plants driven by biological recovery technologies, this review is focused on the generation and re-synthesis of energetic resources and key resources to be recycled as raw materials in a cradle-to-cradle economy concept.

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Section: Resource Recovery For a Circular Economymentioning

confidence: 99%

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

et al. 2017

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“…Lignocellulosic biomass, which is mainly composed of the polysaccharides cellulose and hemicellulose and the polyaromatic compound lignin, is the most abundant renewable and non-edible resource in the world, which can be used as a carbon source to reduce the production cost of microbial lipids (Patel et al, 2016). Several oleaginous yeast species have thus been cultivated on lignocellulose-based hydrolysates (Whiffin et al, 2016). These lignocellulosic raw materials ideally consist of waste from the vicinity and can originate from forestry, agriculture and pulp mills.…”

Section: Introductionmentioning

confidence: 99%

Acetate-detoxification of wood hydrolysates with alkali tolerant Bacillus sp. as a strategy to enhance the lipid production from Rhodosporidium toruloides

Μάτσακας

Novak

Enman

et al. 2017

Bioresource Technology

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The aim of the current work was to convert an acetate-rich hemicellulose liquid fraction (LF) from hot-water extraction of Betula pendula to oils for biodiesel, with Rhodosporidium toruloides. The toxicity of acetate was circumvented by biological detoxification with an isolated alkali-tolerant and acetate-resistant Bacillus sp. strain. Removal of other lignocellulose-derived inhibitors, such as furfural and phenols, was evaluated by two strategies; an activated carbon (AC) treatment of the undiluted LF, and dilution of the LF by 25% (0.75LF) and 50%. (0.50LF). The bacterium consumed most of the acetic acid in 6-8days in the treated or diluted media, which were subsequently used for cultivation of the yeast, for conversion of sugars to oils. The oil concentration reached 2.8 and 1.8g/L, in the AC LF and 0.75LF medium, respectively. In comparison, the oil accumulation in the same media without prior cultivation of Bacillus sp. was 0.86 and 0.03g/L, respectively.

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“…While the lipid profile from microalgae is highly variable, 35 generally, oleaginous yeast produce lipid profiles akin to plant oils with elevated levels of oleic and 36 palmitic acid (Sitepu et al 2014). Recently, we reported on the oleaginous yeast Metschnikowia 37 pulcherrima that can be grown in non-sterile conditions, while having the ability to metabolise a range 38 of oligosaccharide and monosaccharide carbon sources (Whiffin et al 2016;Long et al 2017;Fan et 39 al. 2018).…”

Section: Introduction 31mentioning

confidence: 99%

Enhanced inhibitor tolerance and increased lipid productivity through adaptive laboratory evolution in the oleaginous yeastMetshnikowia pulcherrima

Hicks¹,

Sze

Chuck

et al. 2020

Preprint

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9Microbial lipid production from second generation feedstocks presents a sustainable route to future 10 fuels, foods and bulk chemicals. The oleaginous yeast Metshnikowia pulcherrima has previously been 11 investigated as a potential platform organism for lipid production due to its ability to be grown in non-12 sterile conditions and metabolising a wide range of oligo-and monosaccharide carbon sources 13 within lignocellulosic hydrolysates. However, the generation of inhibitors from depolymerisation 14 causes downstream bioprocessing complications, and despite M. pulcherrima's comparative 15 tolerance, their presence is deleterious to both biomass and lipid formation. Using either a single 16 inhibitor (formic acid) or an inhibitor cocktail (formic acid, acetic acid, fufural and HMF), two strategies 17 of adaptive laboratory evolution were performed to improve M. pulcherrima's fermentation inhibitor 18 tolerance. Using a sequential batch culturing approach, the resulting strains from both strategies had 19 increased growth rates and reduced lag times under inhibiting conditions versus the progenitor. 20Interestingly, the lipid production of the inhibitor cocktail evolved strains markedly increased, with 21 one strain producing 41% lipid by dry weight compared to 22% of the progenitor. The evolved species 22 was cultured in a non-sterile 2L stirred tank bioreactor and accumulated lipid rapidly, yielding 6.1 g/L 23 of lipid (35% cell dry weight) within 48 hours; a lipid productivity of 0.128 g L-1 h-1. Furthermore, the 24 lipid profile was analogous to palm oil, consisting of 39% C16:0 and 56% C18:1 after 48 hours. 25 26

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Toward a microbial palm oil substitute: oleaginous yeasts cultured on lignocellulose

Cited by 41 publications

References 175 publications

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

Resource Recovery from Wastewater by Biological Technologies: Opportunities, Challenges, and Prospects

Acetate-detoxification of wood hydrolysates with alkali tolerant Bacillus sp. as a strategy to enhance the lipid production from Rhodosporidium toruloides

Enhanced inhibitor tolerance and increased lipid productivity through adaptive laboratory evolution in the oleaginous yeastMetshnikowia pulcherrima

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