Tough hydrogel with enhanced tissue integration and in situ forming capability for osteochondral defect repair

Zhou, Feifei; Hong, Yi; Zhang, Xianzhu; Yang, Long; Li, Jun; Jiang, Deming; Bunpetch, Varitsara; Hu, Yin; Ouyang, Hongwei; Zhang, Shufang

doi:10.1016/j.apmt.2018.08.005

Cited by 88 publications

(61 citation statements)

References 58 publications

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“…Human amniotic mesenchymal stromal cells (hAMSCs) cultured in DMEM/F12 with 10% fetal bovine serum and 1% penicillin-streptomycin were used to assess the in vitro cytotoxicity of hydrogels [ 29 ]. The GelMA-imid pre-solution (50 μL) was firstly added in to 96-well plates, then exposed to blue light for 30 s to form hydrogel.…”

Section: Methodsmentioning

confidence: 99%

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Neuro-regenerative imidazole-functionalized GelMA hydrogel loaded with hAMSC and SDF-1α promote stem cell differentiation and repair focal brain injury

Zheng

Chen

et al. 2021

Bioactive Materials

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Brain tissues that are severely damaged by traumatic brain injury (TBI) is hardly regenerated, which leads to a cavity or a repair with glial scarring. Stem-cell therapy is one viable option to treat TBI-caused brain tissue damage, whose use is, whereas, limited by the low survival rate and differentiation efficiency of stem cells. To approach this problem, we developed an injectable hydrogel using imidazole groups-modified gelatin methacrylate (GelMA-imid). In addition, polydopamine (PDA) nanoparticles were used as carrier for stromal-cell derived factor-1 (SDF-1α). GelMA-imid hydrogel loaded with PDA@SDF-1α nanoparticles and human amniotic mesenchymal stromal cells (hAMSCs) were injected into the damaged area in an in-vivo cryogenic injury model in rats. The hydrogel had low module and its average pore size was 204.61 ± 41.41 nm, which were suitable for the migration, proliferation and differentiation of stem cells. In-vitro cell scratch and differentiation assays showed that the imidazole groups and SDF-1α could promote the migration of hAMSCs to injury site and their differentiation into nerve cells. The highest amount of nissl body was detected in the group of GelMA-imid/SDF-1α/hAMSCs hydrogel in the in-vivo model. Additionally, histological analysis showed that GelMA-imid/SDF-1α/hAMSCs hydrogel could facilitate the regeneration of regenerate endogenous nerve cells. In summary, the GelMA-imid/SDF-1α/hAMSCs hydrogel promoted homing and differentiation of hAMSCs into nerve cells, and showed great application potential for the physiological recovery of TBI.

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Section: Methodsmentioning

confidence: 99%

“…The effect of SDF-1α on the migration capacity of hAMSCs was evaluated by scratch wound healing assay [ 29 , 30 ]. The sterilized hydrogel was immersed in DEME/F12 complete medium at 37 °C for 24 h to obtain extract solution.…”

Section: Methodsmentioning

confidence: 99%

Neuro-regenerative imidazole-functionalized GelMA hydrogel loaded with hAMSC and SDF-1α promote stem cell differentiation and repair focal brain injury

Zheng

Chen

et al. 2021

Bioactive Materials

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“…The OHA/HA-ADH and OHA/HA-ADH/N-HACC hydrogels had a white color, while OHA/HA-ADH/O-HACC had a yellow color (Figure 2A). The swelling properties of the hydrogels were investigated to illustrate their water sorption capacity (Zhou et al, 2018). The results revealed that the swelling ratios of the OHA/HA-ADH, OHA/HA-ADH/O-HACC, and OHA/HA-ADH/N-HACC hydrogels in PBS reached 44.47, 6.03, and 10.96, respectively, in the first 2 h (Figure 2B).…”

Section: Physical and Mechanical Properties Of Hydrogelsmentioning

confidence: 99%

Preparation of Antimicrobial Hyaluronic Acid/Quaternized Chitosan Hydrogels for the Promotion of Seawater-Immersion Wound Healing

Wang

Yao

et al. 2019

Front. Bioeng. Biotechnol.

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Wound immersion in seawater with high salt, high sodium, and a high abundance of pathogenic bacteria, especially gram-negative bacteria, can cause serious infections and difficulties in wound repair. The present study aimed to prepare a composite hydrogel composed of hyaluronic acid (HA) and quaternized chitosan (QCS) that may promote wound healing of seawater-immersed wounds and prevent bacterial infection. Based on dynamic Schiff base linkage, hydrogel was prepared by mixing oxidized hyaluronic acid (OHA) and hyaluronic acid-hydrazide (HA-ADH) under physiological conditions. With the addition of quaternized chitosan, oxidized hyaluronic acid/hyaluronic acid-hydrazide/quaternized chitosan (OHA/HA-ADH/O-HACC and OHA/HA-ADH/N-HACC) composite hydrogels with good swelling properties and mechanical properties, appropriate water vapor transmission rates (WVTR), and excellent stability were prepared. The biocompatibility of the hydrogels was demonstrated by in vitro fibroblast L929 cell culture study. The results of in vitro and in vivo studies revealed that the prepared antibacterial hydrogels could largely inhibit bacterial growth. The in vivo study further demonstrated that the antibacterial hydrogels exhibited high repair efficiencies in a seawater-immersed wound defect model. In addition, the antibacterial hydrogels decreased pro-inflammatory factors (TNF-α, IL-1β, and IL-6) but enhanced anti-inflammatory factors (TGF-β1) in wound. This work indicates that the prepared antibacterial composite hydrogels have great potential in chronic wound healing applications, such as severe wound cure and treatment of open trauma infections.

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“…[ 19a,20b,25 ] The intrinsic cell‐adhesion sites, arginine‐glycine‐aspartic acid (RGD) peptides, of gelatin provide a favorable environment for cell attachment and proliferation. [ 26 ] In summary, GelMA MSs provide an excellent cell expansion platform to visualize higher cell viability, attachment rate, proliferation rate, and metabolically activity, compared with Cytodex‐1 MSs.…”

Section: Figurementioning

confidence: 99%

“All‐in‐One” Gel System for Whole Procedure of Stem‐Cell Amplification and Tissue Engineering

Liao

Zhang

et al. 2020

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Mesenchymal stem cells (MSCs) are the most favored cell type presently due to their pleiotropic properties including selfrenewal ability, multidifferentiation potential, and immunoregulation. [1] Adipose-derived mesenchymal stem cells (ADSCs) are frequently used MSCs in clinical trials because of their relative accessibility and widespread ethical acceptability. [2] However, the requirement of cell doses for per treatment commonly Microsphere (MS)-based systems provides great advantages for cell expansion and transplantation due to their high surface-to-volume ratio and biomimetic environment. However, a MS-based system that includes cell attachment, proliferation, passage, harvest, cryopreservation, and tissue engineering together has not been realized yet. An "all-in-one" gel MS-based system is established for human adipose-derived mesenchymal stem cells (hADSCs), realizing real 3D culture with enhanced expansion efficiency and simplified serial cell culture operations, and construction of macrotissues with uniform cell distribution and specific function. A 3D digital light-processing technology is developed to fabricate gel MSs in an effective way. The printed MSs present a suitable environment with rough surface architecture and the mechanical properties of soft tissues, leading to high cell viability, attachment, proliferation, activity, and differentiation potential. Further, convenient standard operation procedures, including cell passage, detachment, and cryopreservation, are established for cell culture on the gel MSs. Finally, hADSCs-loaded gel MSs form macrotissues through a "bottom-up" approach, which demonstrates the potential applications for tissue engineering. These findings exhibit the feasibility and beauty of "allin-one" stem cell culture and tissue engineering system.

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Tough hydrogel with enhanced tissue integration and in situ forming capability for osteochondral defect repair

Cited by 88 publications

References 58 publications

Neuro-regenerative imidazole-functionalized GelMA hydrogel loaded with hAMSC and SDF-1α promote stem cell differentiation and repair focal brain injury

Neuro-regenerative imidazole-functionalized GelMA hydrogel loaded with hAMSC and SDF-1α promote stem cell differentiation and repair focal brain injury

Preparation of Antimicrobial Hyaluronic Acid/Quaternized Chitosan Hydrogels for the Promotion of Seawater-Immersion Wound Healing

“All‐in‐One” Gel System for Whole Procedure of Stem‐Cell Amplification and Tissue Engineering

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