Total reflection X-ray fluorescence spectrometry as a tool for the quantification of gold and platinum metallodrugs: Determination of recovery rates and precision in the ppb concentration range

Meyer, Andreas; Grotefend, Sandra; Gross, Armin; Wätzig, Hermann; Ott, Ingo

doi:10.1016/j.jpba.2012.07.019

Cited by 20 publications

(14 citation statements)

References 20 publications

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“…The relative smallness of the dispersion errors of slurry-TXRF method when pipetting from the same slurry indicates that very reproducible results can be obtained when slurries are sufficiently homogeneous. In agreement with Meyer et al (2012), we also observed that using surfactants (such as Triton X-100 to obtain slightly more homogeneous dried sample spots on TXRF holders) significantly increased the background noise in TXRF spectra, making the quantification of low concentration elements more difficult. Surfactants were therefore not added to slurry samples.…”

Section: Discussionsupporting

confidence: 87%

“…TXRF with Bno or minimal treatment^and direct analysis on powdered samples was used on atmospheric dust collected on TXRF quartz carriers used as impaction plates (Injuk and Van Grieken 1995;Schmeling and Klockow 1997;Esaka et al 2003;Schneider 1989), collected on filters (Borgese et al 2012) or on adhesives (Cantaluppi et al 2013). Finally, slurry sampling for TXRF was applied for biological samples (Meyer et al 2012;De La Calle et al 2012;Wellenreuther et al 2008), drug substances (Shaw et al 2012;Borgese et al 2010), soils (Marguí et al 2010), rocks (Marks et al 2012), and archeological samples (Bonizzoni et al 2013). Overall, Bslurry sampling^appears to be the third most commonly used sample preparation method for TXRF, preceded by digestion and no (or minimal) treatment methods.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of trace metal air pollution in Paris using slurry-TXRF analysis on cemetery mosses

Natali

Zanella

Rankovic

et al. 2016

Environ Sci Pollut Res

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Mosses are useful, ubiquitous accumulation biomonitors and as such can be used for biomonitoring surveys. However, the biomonitoring of atmospheric pollution can be compromised in urban contexts if the targeted biomonitors are regularly disturbed, irregularly distributed, or are difficult to access. Here, we test the hypothesis that cemeteries are appropriate moss sampling sites for the evaluation of air pollution in urban areas. We sampled mosses growing on gravestones in 21 urban and peri-urban cemeteries in the Paris metropolitan area. We focused on Grimmia pulvinata (Hedwig) Smith, a species abundantly found in all studied cemeteries and very common in Europe. The concentration of Al, As, Br, Ca, Ce, Cl, Cr, Cu, Fe, K, Mn, Ni, V, P, Pb, Rb, S, Sr, Ti, and Zn was determined by a total reflection X-ray fluorescence technique coupled with a slurry sampling method (slurry-TXRF). This method avoids a digestion step, reduces the risk of sample contamination, and works even at low sample quantities. Elemental markers of road traffic indicated that the highest polluted cemeteries were located near the highly frequented Parisian ring road and under the influence of prevailing winds. The sites with the lowest pollution were found not only in the peri-urban cemeteries, adjoining forest or farming landscapes, but also in the large and relatively wooded cemeteries located in the center of Paris. Our results suggest that (1) slurry-TXRF might be successfully used with moss material, (2) G. pulvinata might be a good biomonitor of trace metals air pollution in urban context, and (3) cemetery moss sampling could be a useful complement for monitoring urban areas. Graphical abstract We tested the hypothesis that cemeteries are appropriate moss sampling sites for the evaluation of air pollution in urban areas. We sampled 110 moss cushions (Grimmia pulvinata) growing on gravestones in 21 urban and peri-urban cemeteries in the Paris metropolitan area. The concentration of 20 elements in mosses was determined by a total reflection X-ray fluorescence technique coupled with a slurry sampling method. Statistical analysis revealed that: - Urbanized Parisian areas crossed by traffic roads have the highest polluted cemeteries with a strong influence of main wind direction on the distribution of air pollutants - As expected, small cemeteries with low tree density were heavily polluted - Less obvious, large green spaces such as large cemeteries (Père Lachaise, Montmartre, Montparnasse) in the center of a dense metropolis like Paris present the same level of atmospheric trace metal pollution as cemeteries in less urbanized areas or nearing a very large forest. This suggests that even in densely urbanized areas, there is more spatial variability in pollution distribution than usually assumed and that large urban areas with low traffic and green filters such as trees are likely to intercept air pollutants.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Assessment of trace metal air pollution in Paris using slurry-TXRF analysis on cemetery mosses

Natali

Zanella

Rankovic

et al. 2016

Environ Sci Pollut Res

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“…TXRF has also been employed for the determination of Au and Pt containing active ingredients in metallodrugs at the ppb level in a cell suspension. 131 Mercury continues to pose signicant analytical challenges and in one paper the use of a miniaturized micro-plasma based optical emission system has been developed for mercury determination. Photochemical vapour generation (PVG) was coupled with optical emission spectrometry using dielectric barrier discharge (DBD) as an excitation source.…”

Section: Organic Chemicals and Solventsmentioning

confidence: 99%

Atomic spectrometry update: review of advances in the analysis of metals, chemicals and materials

Carter

Clough

Fisher

et al. 2020

J. Anal. At. Spectrom.

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“…The pharmacokinetics of Cisplatin has been studied in the past by determination of the total platinum concentration using atomic absorption spectroscopy (AAS), total reflection X–ray fluorescence (TXRF) 5–7 or inductively coupled plasma-mass spectrometry (ICP-MS) 8, 9 . However, none of these techniques allows for studies on Cisplatin distribution at the organ, cell and subcellular level.…”

Section: Introductionmentioning

confidence: 99%

Elemental bioimaging of Cisplatin in Caenorhabditis elegans by LA-ICP-MS

et al. 2015

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Cis-diamminedichloroplatinum(II) (Cisplatin) is one of the most important and frequently used cytostatic drugs for the treatment of various solid tumors. Herein, a laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) method incorporating a fast and simple sample preparation protocol was developed for the elemental mapping of Cisplatin in the model organism Caenorhabditis elegans (C. elegans). The method allows imaging of the spatially-resolved elemental distribution of platinum in the whole organism with respect to the anatomic structure in L4 stage worms at a lateral resolution of 5 µm. In addition, a dose- and time-dependent Cisplatin uptake was corroborated quantitatively by a total reflection X-ray fluorescence spectroscopy (TXRF) method, and the elemental mapping indicated that Cisplatin is located in the intestine and in the head of the worms. Better understanding of the distribution of Cisplatin in this well-established model organism will be instrumental in deciphering Cisplatin toxicity and pharmacokinetics. Since the cytostatic effect of Cisplatin is based on binding the DNA by forming intra- and interstrand crosslinks, the response of poly(ADP-ribose)metabolism enzyme 1 (pme-1) deletion mutants to Cisplatin was also examined. Loss of pme-1, which is the C. elegans ortholog of human poly(ADP-ribose) polymerase 1 (PARP-1) led to disturbed DNA damage response. With respect to survival and brood size, pme-1 deletion mutants were more sensitive to Cisplatin as compared to wildtype worms, while Cisplatin uptake was indistinguishable.

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Total reflection X-ray fluorescence spectrometry as a tool for the quantification of gold and platinum metallodrugs: Determination of recovery rates and precision in the ppb concentration range

Cited by 20 publications

References 20 publications

Assessment of trace metal air pollution in Paris using slurry-TXRF analysis on cemetery mosses

Assessment of trace metal air pollution in Paris using slurry-TXRF analysis on cemetery mosses

Atomic spectrometry update: review of advances in the analysis of metals, chemicals and materials

Elemental bioimaging of Cisplatin in Caenorhabditis elegans by LA-ICP-MS

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