Total reflection X-ray fluorescence as a fast multielemental technique for human placenta sample analysis

Marguí, Eva; Ricketts, Phylicia; Fletcher, Horace; Karydas, A. G.; Migliori, Alessandro; Leani, Juan José; Hidalgo, Manuela; Queralt, I.; Voutchkov, Mitko

doi:10.1016/j.sab.2017.02.008

Cited by 25 publications

(18 citation statements)

References 19 publications

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“…However, for the latter, no validation experiments are given. In contrast, Marguí et al [21] compared sample preparation by digestion or suspension after drying and grinding of human placenta and validated the method by use of reference material and measurements by ICP-OES. These authors observed systematic minor recoveries in the suspensions of the real samples, which were then corrected using the obtained factors.…”

Section: Analytical Figures Of Merit and Comparison With Other Methodsmentioning

confidence: 99%

“…The feasibility of such approach has recently been confirmed by Espinoza-Quiñones et al [38] who investigated several matrix reference materials applying solid suspensions prepared by using pure water. Agreeable precision and accuracy, as well as sufficient limits of detection for trace and major elements, were obtained by TXRF analysis, whereas Marguí et al [21] found minor recoveries in comparison with ICP-OES analysis of digests when suspending dried and grinded human placenta in surfactant-containing water for TXRF analysis. Anyway, the studied reference materials and the placenta samples were ideally homogenized and dried materials, whereas the fresh, real samples investigated in this work (sections of mouse liver and cell samples) were not pre-homogenized, as any manipulation of the minute sample amounts was avoided.…”

Section: Optimization Of Sample Preparationmentioning

confidence: 99%

“…Examples for valid trace determination by TXRF cover a broad variety of different biological samples, from isolated proteins and nuclei acids to fluids, cells, bacteria, hair, and tissues such as the liver, placenta, chest, kidney, lung, prostate, or colon [2,13,[20][21][22][23][24][25][26][27][28][29][30][31][32][33]. Besides the above-mentioned advantages, highly convenient and reliable multi-element quantification can be performed by adding an internal standard for a one-point calibration [20,21,27,30,31]. In addition, trace element determination by TXRF requires only minute sample amounts providing detection limits in the picogram range [19,30].…”

Section: Introductionmentioning

confidence: 99%

“…In recent times, total reflection X-ray fluorescence spectrometry (TXRF) is increasingly proposed in trace element determination in biological samples as it combines multi-element determination with the possibility of applying suspensions and/or solids [ 19 – 21 ]. Furthermore, TXRF is cost-effective and does not require any gas or cooling media.…”

Section: Introductionmentioning

confidence: 99%

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Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples

et al. 2020

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Trace elements are essential for life and their concentration in cells and tissues must be tightly maintained and controlled to avoid pathological conditions. Established methods to measure the concentration of trace elements in biological matrices often provide only single element information, are time-consuming, and require special sample preparation. Therefore, the development of straightforward and rapid analytical methods for enhanced, multi-trace element determination in biological samples is an important and raising field of trace element analysis. Herein, we report on the development and validation of a reliable method based on total reflection X-ray fluorescence (TXRF) analysis to precisely quantify iron and other trace metals in a variety of biological samples, such as the liver, parenchymal and non-parenchymal liver cells, and bone marrow-derived macrophages. We show that TXRF allows fast and simple one-point calibration by addition of an internal standard and has the potential of multi-element analysis in minute sample amounts. The method was validated for iron by recovery experiments in homogenates in a wide concentration range from 1 to 1600 μg/L applying well-established graphite furnace atomic absorption spectrometry (GFAAS) as a reference method. The recovery rate of 99.93 ± 0.14% reveals the absence of systematic errors. Furthermore, the standard reference material "bovine liver" (SRM 1577c, NIST) was investigated in order to validate the method for further biometals. Quantitative recoveries (92-106%) of copper, iron, zinc, and manganese prove the suitability of the developed method. The limits of detection for the minute sample amounts are in the low picogram range. Keywords Total reflection X-ray fluorescence spectrometry. Iron trace analysis. Biometal trace analysis. Bone marrow-derived macrophages. Liver cells. Liver tissues Published in the topical collection featuring Female Role Models in Analytical Chemistry.

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Section: Analytical Figures Of Merit and Comparison With Other Methodsmentioning

confidence: 99%

Section: Optimization Of Sample Preparationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples

et al. 2020

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“…Biological and clinical materials generally need to be pretreated to remove the organic matrix which would increase the background signal and thus worsen the detection limits. Common pretreatment methods are ashing, acid digestion, or purification using chromatographic methods [42][43][44][45][46][47][48][49][50]. Mineral oil, vegetable oil or fatty acid containing samples like creams and lotions can be diluted with a solvent or if necessary subjected to cold oxygen plasma ashing to remove excess carbon.…”

Section: Biological and Food Applicationsmentioning

confidence: 99%

Total reflection X-ray fluorescence

Schmeling

2019

Physical Sciences Reviews

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Abstract Total reflection X-ray fluorescence (TXRF) spectrometry is a non-destructive and surface sensitive multi-element analytical method based on energy dispersive X-ray fluorescence spectrometry with detection limits in the lower picogram range. It utilizes the total reflection of the primary X-ray beam at or below the critical angle of incidence. At this angle, the fluorescence intensity is substantially enhanced for samples present as small granular residue or as thin homogenous layer deposited at the surface of a thick substrate. Generally, two types of application exist: micro- and trace-analysis as well as surface and thin-layer analysis. For micro- and trace-analysis, a small amount of the solid or liquid sample is deposited on an optically flat substrate, typically quartz or polycarbonate. The dried residue is analyzed at a fixed angle setting slightly below the critical angle. Quantification is carried out by means of internal standardization. For surface and thin-layer analysis, the surface of an optically flat substrate is scanned. Variations of the incident angle of the primary X-ray beam provide information about the type and sometimes also the amount of material present at or slightly below the surface of the substrate. Major fields of application are environmental samples, biological tissues, objects of cultural heritage, semiconductors and thin-layered materials and films.

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Potential of total‐reflection X‐ray spectrometry for multielement analysis of biological samples using dilution or suspension sample preparation techniques

et al. 2021

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Total reflection X-ray fluorescence as a fast multielemental technique for human placenta sample analysis

Cited by 25 publications

References 19 publications

Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples

Total reflection X-ray fluorescence spectrometry for trace determination of iron and some additional elements in biological samples

Total reflection X-ray fluorescence

Potential of total‐reflection X‐ray spectrometry for multielement analysis of biological samples using dilution or suspension sample preparation techniques

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