Total Protein, Deoxyribonucleic Acid, Ribonucleic Acid, and Histone Content of Isolated Nuclei of Chicken Erythrocytes

Abstract

Nuclei were isolated from erythroid cells of thirteen day chick embryos, eighteen day chick embryos, and hens; total protein, DNA, RNA, and histone contents were determined per nucleus. The average picogram quantities of total protein, DNA, RNA, and histone per nucleus from erythrocytes of the hen were 3.20, 2.05, 0.33, and 1.74, respectively. The results were the same in nuclei from the erythrocytes of the two ages of embryos except that the average total protein in nuclei from 18-day embryos was 2.64 picograms. ABSTRACTThe egg characteristics of shell thickness, specific gravity, egg weight, width/length ratio and tint of the brown shell eggs were regressed on the shell strength measurements of non-destructive deformation and breaking strength. Six commercially available laying strains (3 white egg and 3 brown egg) were used to determine differences between